Cinnamoyl compound and use of the same

ABSTRACT

The present invention relates to a cinnamoyl compound represented by the formula (I):

This application is a U.S. National Stage of International Application No. PCT/JP2004/014006 filed Sep. 16, 2004.

TECHNICAL FIELD

The present invention relates to a cinnamoyl compound and use of the same.

BACKGROUND ART

In diseases and disorders such as hepatic cirrhosis, interstitial pulmonary disease, chronic renal failure (or disease resulting in chronic renal failure), hyperplasia scar after inflammation, postoperative scars or burn scars, scleroderma, arteriosclerosis, hypertension and the like, excessive accumulation of an extracellular matrix, a representative of which is collagen, causes fibrosis and sclerosis of tissues, resulting in decreased functions, cicatrization and the like in the organs or tissues. Such excessive accumulation of an extracellular matrix is induced by increased production of collagen due to a breakdown of balance between biosynthesis and degradation of collagen and the like. In fact, it has been observed that expression of a collagen gene, in particular, a Type I collagen gene has been increased in a fibrotic tissue [e.g. J. Invest. Dermatol., 94, 365, (1990) and Proc. Natl. Acad. Sci. USA, 88, 6642, (1991)]. It has been also observed that the amount of TGF-β, which is a cytokine, has been increased in a fibrotic tissue [e.g. J. Invest. Dermatol., 94, 365, (1990) and Proc. Natl. Acad. Sci. USA, 88, 6642, (1991)]. It has been shown that TGF-β has increased expression of a Type I collagen gene and been involved in increased production of collagen and, consequently, fibrosis of a tissue [e.g. Lab. Invest., 63, 171, (1990) and J. Invest. Dermatol., 94, 365, (1990)]. It has been also shown that by administering an anti-TGF-β antibody or a soluble anti-TGF-β receptor to a model animal of tissue fibrosis, improvement of tissue fibrosis has been achieved and thereby the tissue function has been also improved [e.g. Diabetes, 45, 522-530, (1996), Proc. Natl. Acad. Sci. USA, 96, 12719-12724, (1999) and Proc. Natl. Acad. Sci. USA, 97, 8015-8020, (2000)]. It has been also known that by administering a compound which suppressively acts on intracellular signal transduction via TGF-β, improvement in fibrosis of a tissue has been achieved and thereby the tissue function has been also improved [e.g. Autoimmunity, 35, 277-282, (2002), J. Hepatol., 37, 331-339, (2002) and Life Sci., 71, 1559-1606, (2002)].

Thus, there is a need for development and provision of a drug which improves fibrosis of a tissue by decreasing expression of a Type I collagen gene in the tissue to reduce accumulation of collagen (i.e. a collagen accumulation-suppressing agent and a fibrosing disease-treating agent).

DISCLOSURE OF INVENTION

Under such circumstances, the present inventors intensively studied, and as a result, found that compounds represented by the following formulas (I) to (XXXVIII) have the ability to suppress transcription of a Type I collagen gene, which led to the present invention.

That is, the present invention provides:

1. A cinnamoyl compound represented by the formula (I):

wherein, I. A represents a benzene ring or a pyridine ring; and in (Y_(α))_(q), Y_(α) is a substituent on a carbon atom and represents a group included in the following X₀ group or Y₀ group, q represents 0, 1, 2, 3 or 4, and Y_(α)s are the same or different when q is 2 or more and the adjacent two same or different Y_(α)s together may form a group included in the Z₀ group to be fused to the A ring when q is 2 or more; and in (X_(α))_(p), X_(α) represents a substituent on a carbon atom which does not belong to the following X₀ group, Y₀ group and Z₀ group, p represents 1, 2, 3, 4 or 5, and X_(α)s may be the same or different when p is 2 or more; and the sum of p and q is 5 or less;

(1) the X₀ group: a M_(a)- group, wherein M_(a) represents a R_(b)— group (wherein R_(b) represents a C1-C10 alkyl group optionally substituted with a halogen atom), a halogen atom, a nitro group, a cyano group, a hydroxy group, a R_(c)—B_(a)—R_(d)- group (wherein R_(c) represents a C1-C10 alkyl group optionally substituted with a halogen atom, B_(a) represents an oxy group, a thio group, a sulfinyl group or a sulfonyl group, and R_(d) represents a single bond or a C1-C10 alkylene group), a HOR_(d)— group (wherein R_(d) is as defined above), a R_(e)—CO—R_(d)— group (wherein R_(e) represents a hydrogen atom, or a C1-C10 alkyl group optionally substituted with a halogen atom, and R_(d) is as defined above), a R_(e)—CO—O—R_(d)— group (wherein R_(e) and R_(d) are as defined above), a R_(e)O—CO—R_(d)— group (wherein R_(e) and R_(d) are as defined above), a HO—CO—CH═CH— group, a R_(e)R_(e)′N—R_(d)— group (wherein R_(e) and R_(e)′ are the same or different, R_(e) is as defined above, R_(e)′ has the same meaning as R_(e) has, and R_(d) is as defined above), a R_(e)—CO—NR_(e)—R_(d)— group (wherein R_(e), R_(e)′ and R_(d) are as defined above), a R_(b)O—CO—N(R_(e))—R_(d)— group (wherein R_(b), R_(e) and R_(d) are as defined above), a R_(e)R_(e)′N—CO—R_(d)— group (wherein R_(e), R_(e)′ and R_(d) are as defined above), a R_(e)R_(e)′N—CO—NR_(e)″—R_(d)— group (wherein R_(e), R_(e)′ and R_(e)″ are the same or different, R_(e) and R_(e)′ are as defined above, R_(e)″ has the same meaning as R_(e) has, and R_(d) is as defined above), a R_(e)R_(e)′N—C(′NR_(e)″)—NR_(e)′″—R_(d)— group (wherein R_(e), R_(e)′, R_(e)″ and R_(e)′″ are the same or different, R_(e), R_(e)′ and R_(e)″ are as defined above, R_(e)′″ has the same meaning as R_(e) has, and R_(d) is as defined above), a R_(b)—SO₂—NR_(e)—R_(d)— group (wherein R_(b), R_(e) and R_(d) are as defined above), a R_(e)R_(e)′N—SO₂—R_(d)— group (wherein R_(e), R_(e)′ and R_(d) are as defined above), a C2-C10 alkenyl group or a C2-C10 alkynyl group;

(2) the Y₀ group: a M_(b0)-R_(d)— group, wherein M_(b0) represents a M_(c0)- group

[wherein M_(co) represents a M_(do)-R_(d)′— group [wherein M_(do) represents a 6 to 10-membered aryl group optionally substituted with a M_(a)- group (wherein M_(a) is as defined above), a 5 to 10-membered heteroaryl group optionally substituted with a M_(a)- group (wherein M_(a) is as defined above), a 3 to 10-membered cyclic hydrocarbon or heterocyclic group optionally substituted with a M_(a)- group (wherein M_(a) is as defined above) and optionally containing an unsaturated bond, a (b₀)- group

(in the (b₀)- group, G₀ forms an optionally substituted, saturated or unsaturated, nonaromatic 5 to 14-membered cyclic hydrocarbon or heterocyclic ring), a (c₀)- group

(in the (C₀)— group, J₀ forms a 5 to 7-membered aromatic ring optionally containing a nitrogen atom), a (d₀)- group

[wherein d₀ forms a 5 to 12-membered hydrocarbon ring which is substituted with a carbonyl group or a thiocarbonyl group and further which may be optionally substituted with an oxy group, a thio group, a —NR₁— group {wherein R₁ represents a hydrogen atom, a C1-C10 alkyl group, a C2-C10 alkyl group substituted with a halogen atom or a R₂—- B₁— group (wherein R₂ represents a C1-C10 alkyl group, a C3-C10 alkenyl group or a C3-C10 alkynyl group, and B₁ represents an oxy group, a thio group, a sulfinyl group or a sulfonyl group), a C3-C10 alkenyl group, or a C3-C10 alkynyl group}, a sulfinyl group or a sulfonyl group] or a (e₀)-group

{wherein e₀ forms a 5 to 12-membered hydrocarbon ring optionally substituted with a carbonyl group, a thiocarbonyl group, an oxy group, a thio group, a —NR₁— group (wherein R₁ is as defined above), a sulfinyl group or a sulfonyl group}; and R_(d)′ is the same as or different from R_(d) and has the same meaning as R_(d) has]], a M_(c0)-B_(a)— group (wherein M_(c0) and B_(a) are as defined above), a M_(c0)-CO— group (wherein M_(c0) is as defined above), a M_(c0)-CO—O— group (wherein M_(c0) is as defined above), a M_(c0)O—CO— group (wherein M_(c0) is as defined above), a M_(c0)R_(e)N— group (wherein M_(c0) and R_(e) are as defined above), a M_(c0)-CO—NR_(e)— group (wherein M_(c0) and R_(e) are as defined above), a M_(c0)O—CO—NR_(e)— group (wherein M_(c0) and R_(e) are as defined above), a M_(c0)R_(e)N—CO— group (wherein M_(c0) and R_(e) are as defined above), a M_(c0)R_(e)N—CO—NR_(e)′— group (wherein M_(c0), R_(e) and R_(e)′ are as defined above), a M_(c0)R_(e)N—C(═NR_(e)′)—NR_(e)″— group (wherein M_(c0), R_(e), R_(e)′ and R_(e)″ are as defined above), a M_(c0)-SO₂—NR_(e)— group (wherein M_(c0) and R_(e) are as defined above) or a M_(c0)R_(e)N—SO₂— (wherein M_(c0) and R_(e) are as defined above), and R_(d) is as defined above;

(3) the Z₀ group: a 5 to 12-membered cyclic hydrocarbon or heterocyclic ring optionally substituted with a halogen atom, a C1-C10 alkoxy group, a C3-C10 alkenyloxy group, a C3-C10 alkynyloxy group, a carbonyl group, a thiocarbonyl group, an oxy group, a thio group, a sulfinyl group or a sulfonyl group, which is an aromatic or nonaromatic and monocyclic or fused ring and which is fused to the A ring;

II. Q_(α) represents an optionally substituted hydroxy group, or an optionally substituted amino group;

III. T_(α) represents a hydrogen atom, or a substituent on the nitrogen atom; and

IV. K_(α) and L_(α) are the same or different and represent a hydrogen atom or a substituent on a carbon atom, or K_(α) and L_(α) together may form an optionally substituted C1-C10 alkylene group or an optionally substituted C1-C10 alkenylene group; and

the term “as defined above” used for the same symbols among plural substituents means that the plural substituents independently represent the same meaning as that described above and, among the plural substituents, although the selection range of substituents to be selected is the same, selected substituents may be the same or different as long as they are selected within the range;

2. A cinnamoyl compound represented by the formula (II):

wherein, I. A represents a benzene ring or a pyridine ring; II. in (X_(A0))_(p), X_(A0) is a substituent on a carbon atom and represents a group included in any group of the following A₀ to N₀ groups, p represents 1, 2, 3, 4 or 5, and when p is 2 or more, X_(A0)s are the same or different;

(1) the A₀ group:

a D₁-R₄— group [wherein D₁ represnts a (R₁—(O)_(k)—)A₁N—(O)_(k′)— group [wherein R₁ represents a hydrogen atom, or a C1-C10 alkyl group, or a C2-C10 alkyl group substituted with a halogen atom or a R₂—B₁— group (wherein R₂ represents a C1-C10 alkyl-group, a C3-C10 alkenyl group or a C3-C10 alkynyl group, and B₁ represents an oxy group, a thio group, a sulfinyl group or a sulfonyl group), or a C3-C10 alkenyl group, or a C3-C10 alkynyl group, k represents 0 or 1, A₁ represents a R₃—(CHR₀)_(m)—(B₂—B₃)_(m′)— group {wherein R₃ represents a hydrogen atom, or a C1-C10 alkyl group optionally substituted with a halogen atom or a R₂—B₁— group (wherein R₂ and B₁ are as defined above), or a C2-C10 alkenyl group, or a C2-C10 alkynyl group, R₀ represents a hydrogen atom, a C1-C10 alkyl group or a C2-C10 haloalkyl group, m represents 0 or 1, B₂ represents a single bond, an oxy group, a thio group or a —N((O)_(n)R₁′)— group (wherein R₁′ is the same as or different from R₁, and has the same meaning as R₁ has, and n represents 0 ro 1), B₃ represents a carbonyl group, a thiocarbonyl group or a sulfonyl group, m′ represents 0 or 1, and when B₃ is a sulfonyl group, it does not occur that m is 0 and R₃ is a hydrogen atom at the same time}, and k′ represents 0 or 1], and R₄ represents a C1-C10 alkylene group, provided that a R₀′R₀′N—R₄— group (wherein R₀′ and R₀″ are the same as or different from R₀ and have the same meaning as R₀ has, and R₄ is as defined above) is excluded],

a D₂-R₄— group [wherein D₂ represents a cyano group, a R₁R₁′NC(═N—(O)_(n)-A₁)-group (wherein R₁, R₁′, n and A₁ are as defined above), an A₁N═C(—OR₂)— group (wherein A₁ and R₂ are as defined above) or a NH₂—CS— group, and R₄ is as defined above],

a D₃-R₄— group [wherein D₃ represents a nitro group or a R₁OSO₂— group (wherein R₁ is as defined above), and R₄ is as defined above], or

a R₁OSO₂— group [wherein R₁ is as defined above];

(2) the B₀ group: an (a₀)-group

in the (a₀)-group, E₀ forms an optionally substituted, saturated or unsaturated, aromatic or nonaromatic 5 to 14-membered cyclic hydrocarbon or heterocyclic ring, and R₁ is as defined above;

(3) the C₀ group: a C2-C10 alkenyl group substituted with a halogen atom, a R₂—B₁— group (wherein R₂ and B₁ are as defined above), a D₄-R₄— group [wherein D₄ represents a hydroxy group or an A₁-O— group (wherein A₁ is as defined above), and R₄ is as defined above], a D₅- group [wherein D₅ represents a O═C(R₃)— group (wherein R₃ is as defined above), an A₁-(O)_(n)—N═C(R₃)— group (wherein A₁, n and R₃ are as defined above), a R₁—B₀—CO—R₄—(O)_(n)—N═C(R₃)— group {wherein R₁, R₄, n and R₃ are as defined above, and B₀ represents an oxy group, a thio group or a —N((O)_(m)R₁′)— group (wherein R₁′ and m are as defined above)}, a D₂-R₄—(O)_(n)—N′C(R₃)— group (wherein D₂, R₄, n and R₃ are as defined above) or a R₁A₁N—N═C(R₃)— group (wherein R₁, A₁ and R₃ are as defined above)], a R₁A₁N—O—R₄— group (wherein R₁, A₁ and R₄ are as defined above), a R₁(A₁-(O)_(n)—)N— group (wherein R₁, A₁ and n are as defined above), a D₂- group (wherein D₂ is as defined above) or a D₃- group (wherein D₃ is as defined above);

(4) the D₀ group: a C2-C10 alkynyl group substituted with a (b₀)-R₄— group (in (b₀)

G₀ forms an optionally substituted, saturated or unsaturated, nonaromatic 5 to 14-membered cyclic hydrocarbon or heterocyclic ring), a (c₀)-R₄— group (in (c₀)

J₀ forms an aromatic 5 to 7-membered ring optionally containing a nitrogen atom and R₄ is as defined above), a halogen atom, a R₂—B₁—R₄— group (wherein R₂, B₁ and R₄ are as defined above), a D₄-R₄— group (wherein D₄ and R₄ are as defined above), a D₅- group (wherein D₅ is as defined above), a D₁-R₄— group (wherein D₁ and R₄ are as defined above), a D₂- group (wherein D₂ is as defined above) or a D₃-R₄— group (wherein D₃ and R₄ are as defined above);

(5) the E₀ group: an A₂-CO—R₅— group, provided that R₅ is not a vinylene group when A₂ is a hydroxy group, wherein A₂ represents

(i) an A₃-B₄— group

wherein A₃ represents a hydrogen atom, or a C1-C10 alkyl group, or a C2-C10 haloalkyl group, or a C2-C10 alkenyl group optionally substituted with a halogen atom, or a C3-C10 alkynyl group optionally substituted with a halogen atom, or a R_(a0)—(R₄)_(m)— group (wherein R_(a0) represents an optionally substituted 5 to 7-membered aryl group or heteroaryl group, and R₄ and m are as defined above), or a C1-C10 alkyl group substituted with a (b₀)-R₄— group (wherein (b₀) and R₄ are as defined above), a (c₀)-R₄— group (wherein (c₀) and R₄ are as defined above), a R₂—B₁—R₄— group (wherein R₂, B₁ and R₄ are as defined above), a D₄-R₄— group (wherein D₄ and R₄ are as defined above), a D₅- group (wherein D₅ is as defined above), a D₁-R₄— group (wherein D₁ and R₄ are as defined above), a D₂- group (wherein D₂ is as defined above), a D₃-R₄— group (wherein D₃ and R₄ are as defined above) or an A₄-SO₂—R₄— group {wherein A₄ represents a (b₀)-group (wherein (b₀) is as defined above), a (c₀)-group (wherein (c₀) is as defined above) or a R₁R₁N— group (wherein R₁ and R₁′ are as defined above), and R₄ is as defined above}, and

B₄ represents an oxy group, a thio group or a —N((O)_(m)R₁)— group (wherein R₁ and m are as defined above), provided that A₃ is not a hydrogen atom when B₄ is a thio group;

(ii) a R₁—B₄—CO—R₄—B₄′— group, wherein R₁, B₄ and R₄ are as defined above, B₄′ is the same as or different from B₄ and has the same meaning as B₄ has, provided that R₂ is not a hydrogen atom when B₄ is a thio group, or

a D₂-R₄—B₄— group, wherein D₂, R₄ and B₄ are as defined above;

(iii) a R₂—SO₂—NR₁— group, wherein R₂ is as defined above, provided that a hydrogen atom is excluded, and R₁ is as defined above;

(iv) a (b₀)-group, wherein (b₀) is as defined above;

(v) a (c₀)-group, wherein (c₀) is as defined above; or

(vi) a R₁A₁N—NR₁′— group, wherein R₁, A₁ and R₁′ are as defined above; and

R₅ represents a C2-C10 alkenylene group optionally substituted with a halogen atom or a C2-C10 alkynylene group;

(6) the F₀ group: an A₅-B₅—R₆— group

wherein A₅ represents a C2-C10 alkyl group substituted with a D₄- group (wherein D₄ is as defined above), a D₁- group (wherein D₁ is as defined above), a D₃- group (wherein D₃ is as defined above) or an A₄-SO₂— group (wherein A₄ is as defined above), or a C1-C10 alkyl group substituted with a R₂—B₁— group (wherein R₂ and B₁ are as defined above), a D₂- group (wherein D₂ is as defined above), a D₅- group (wherein D₅ is as defined above) or an A₂-CO— group (wherein A₂ is as defined above),

B₅ represents a B₁— group (wherein B₁ is as defined above) or a —NA₁- group (wherein A₁ is as defined above), and

R₆ represents a single bond or a C1-C10 alkylene group;

(7) the G₀ group: an A₆-B₅—R₆— group

wherein A₆ represents an (a₀)-R₄— group (wherein (a₀) and R₄ are as defined above), or a C2-C10 alkenyl group, or a C2-C10 alkynyl group, or a C2-C10 alkenyl group substituted with a halogen atom, a R₂—B₁— group (wherein R₂ and B₁ are as defined above), a D₅- group (wherein D₅ is as defined above), a D₂- group (wherein D₂ is as defined above) or an A₂-CO— group (wherein A₂ is as defined above), or a C2-C10 alkynyl group substituted with a halogen atom, a R₂—B₁— group (wherein R₂ and B₁ are as defined above), a D₅- group (wherein D₅ is as defined above), D₂- group (wherein D₂ is as defined above) or an A₂-CO— group (wherein A₂ is as defined above), or a C3-C10 alkenyl group substituted with a (b₀)-group (wherein (b₀) is as defined above), a (c₀)-group (wherein (c₀) is as defined above), a D₄- group (wherein D₄ is as defined above), a D₁- group (wherein D₁ is as defined above) or a D₃- group (wherein D₃ is as defined above), or a C3-C10 alkynyl group substituted with a D₄- group (wherein D₄ is as defined above), a D₁- group (wherein D₁ is as defined above) or a D₃- group (wherein D₃ is as defined above), and

-   -   B₅ and R₆ are as defined above;

(8) the H₀ group:

a D₂-N(—(O)_(n)-A₁)-R₆— group (wherein D₂, n, A₁ and R₆ are as defined above),

a D₂- group (wherein D₂ is as defined above, provided that a cyano group is excluded),

a R₁(R₁′(O)_(n))N—CR₁″═N—R₆— group (wherein R₁, R₁′, n and R₆ are as defined above, R¹″ is the same as or different from R₁ and has the same meaning as that of R₁),

a R₁—(O)_(n)—N═CR₁′—NR₂—R₆— group (wherein R₁, n, R₁′, R₂ and R₆ are as defined above),

a R₂—B₃—NR₁—CO—NR₁′—R₆— group (wherein R₂, B₃, R₁, R₁′ and R₆ are as defined above),

a D₂-CO—NR₁—R₆— group (wherein D₂, R₁ and R₆ are as defined above) or

an A₂-COCO—NR₁—R₆— group (wherein A₂, R₁ and R₆ are as defined above);

(9) the I₀ group:

an A₇-B₆—N((O)R₁)—R₆— group [wherein A₇ represents a C2-C10 alkenyl group optionally substituted with a halogen atom, or a C2-C10 alkynyl group, or a C3-C10 haloalkynyl group, or a R₂—B₁—R₄— group (wherein R₂, B₁ and R₄ are as defined above), or a D₄-R₄— group (wherein D₄ and R₄ are as defined above), or a D₁-R₄— group (wherein D₁ and R₄ are as defined above), or a (b₀)-R₄— group (wherein (b₀) and R₄ are as defined above), or a (c₀)-R₄— group (wherein (c₀) and R₄ are as defined above), or a D₂-R₄— group (wherein D₂ and R₄ are as defined above), or a D₃-R₄— group (wherein D₃ and R₄ are as defined above), or an A₄-SO₂—R₄— group (wherein A₄ and R₄ are as defined above), or an A₂-CO—R₄— group (wherein A₂ and R₄ are as defined above), B₆ represents a carbonyl group or a thiocarbonyl group, and n, R₁ and R₆ are as defined above],

an A₈-CS—N((O)_(n)R₁)—R₆— group [wherein A₈ represents a hydrogen atom or a C1-C10 alkyl group optionally substituted with a halogen atom, and n, R₁ and R₄ are as defined above],

an A₇′-B₂′—B₃—N((O)_(n)R₁)—R₆— group [wherein A₇′ represents a C3-C10 alkenyl group optionally substituted with a halogen atom, or a C3-C10 alkynyl group optionally substituted with a halogen atom, or a R₂—B₁—R₄′— group (wherein R₂ and B₁ are as defined above, and R₄′ represents a C2-C10 alkylene group), or a D₄-R₄′— group (wherein D₄ and R₄′ are as defined above), or a D₁-R₄′— group (wherein D₁ and R₄′ are as defined above), or a (b₀)-R₄′— group (wherein (b₀) and R₄— are as defined above), or a (c₀)-R₄′— group (wherein (c₀) and R₄′ are as defined above), or a D₂-R₄— group (wherein D₂ and R₄ are as defined above), or a D₃-R₄′— group (wherein D₃ and R₄′ are as defined above), or an A₂-CO—R₄— group (wherein A₂ and R₄ are as defined above), B₂′ represents an oxy group, a thio group or a —N((O)_(n′)R₁′)— group (wherein n′ is the same as or different from n and has the same meaning as that of n, and R₁′ is as defined above), and B₃, n, R₁ and R₆ are as defined above],

an A₈′-B₂′—CS—N((O)_(n)R₁)—R₆— group [wherein A₈′ represents a C1-C10 alkyl group or a C2-C10 haloalkyl group, B₂′ is as defined above, and n, R₁ and R₆ are as defined above],

an A₈′-S—B₃′—N((O)_(n)R₁)—R₆— group [wherein A₈′, n, R₁ and R₆ are as defined above, and B₃′ represents a carbonyl group or a sulfonyl group] or

an A₇″-SO₂—N((O)_(n)R₁)—R₆— group [wherein A₇″ represents a C2-C10 alkenyl group, or a C3-C10 alkenyl group substituted with a halogen atom, or a C3-C10 alkynyl group optionally substituted with a halogen atom, or a R₂—B₁—R₄′— group (wherein R₂, B₁ and R₄′ are as defined above), or a D₄-R₄′— group (wherein D₄ and R₄′ are as defined above), or a D₅-R₄— group (wherein D₅ and R₄ are as defined above), or a D₁-R₄′— group (wherein D₁ and R₄′ are as defined above), or a (b₀)-R₄′— group (wherein (b₀) and R₄′ are as defined above), or a (c₀)-R₄′— group (wherein (c₀) and R₄′ are as defined above), or a D₂-R₄— group (wherein D₂ and R₄ are as defined above), or a NO₂—R₄— group (wherein R₄ is as defined above), or an A₂-CO—R₄— group (wherein A₂ and R₄ are as defined above), and n, R₁ and R₆ are as defined above];

(10) the J₀ group:

an A₇-CO— group (wherein A₇ is as defined above),

an A₉-CS— group (wherein A₉ represents A₇ or A₈),

an A₉′ (O)_(m)N═C(A₉)-group (wherein A₉′ represents A₇′ or A₈′, and m and A₉ are as defined above),

a D₂-CO— group (wherein D₂ is as defined above),

an A₂-COCO— group (wherein A₂ is as defined above),

an A₉-CO—B₁′—R₆— group (wherein A₉ and R₆ are as defined above, and B₁′ represents an oxy group or a thio group, provided that A₉ is not A₈ when B₁′ is an oxy group),

an A₉-CS—B₁′—R₆— group (wherein A₉, B₁′ and R₆ are as defined above),

an A₇″-SO₂—B₁′—R₆— group (wherein A₇″, B₁′ and R₆ are as defined above),

an A₈-SO₂—B₁′—R₆— group (wherein A₈, B₁′ and R₆ are as defined above, provided that A₈ is not a hydrogen atom),

an A₉′-B₂′—B₃—B₁′—R₆— group (wherein A₉′, B₂′, B₃, B₁′ and R₆ are as defined above), or

a C2-C10 alkenyl group substituted with a (b₀)-group (wherein (b₀) is as defined above) or a (c₀)-group (wherein (c₀) is as defined above);

(11) the K₀ group: an A₁₀-N((O)_(n)R₁)—CO—R₆— group

wherein A₁₀ represents a hydrogen atom (provided that n is not 0), an A₇″-SO₂— group (wherein A₇″ is as defined above), an A₈-SO₂— group (wherein A₈ is as defined above, provided that A₈ is not a hydrogen atom), an A₉′O— group (wherein A₉′ is as defined above, provided that n is not 1), an A₉′-group (wherein A₉′ is as defined above, provided that A₈′ is excluded when n is 0), a R₂OCH₂— group (wherein R₂ is as defined above), an A₂-CO—R₄— group (wherein A₂ and R₄ are as defined above) or an A₂-CO—CH(CH₂CO-A₂)-group (wherein A₂ is as defined above), and n, R₁ and R₆ are as defined above;

(12) the L₀ group:

an A₁₀′-N((O)_(n)R₁)—SO₂—R₆— group [wherein A₁₀′ represents a hydrogen atom (provided that n is not 0), an A₉′O— group (wherein A₉′ is as defined above, provided that n is not 1), an A₉′-group (wherein A₉′ is as defined above, provided that A₈′ is excluded when n is 0), a R₂—CO— group (wherein R₂ is as defined above), an A₂-CO—R₄— group (wherein A₂ and R₄ are as defined above) or an A₂-CO—CH(CH₂CO-A₂)-group (wherein A₂ is as defined above), and n, R₁ and R₆ are as defined above],

an A₉″R₁N—SO₂—N((O)_(n)R₁′)—R₆— group [wherein A₉″ represents a hydrogen atom or an A₉′-group (wherein A₉′ is as defined above), and R₁, n, R₁′ and R₆ are as defined above] or

a (b₀)-SO₂—N((O)_(n)R₁′)—R₆— group [wherein (b₀), n, R₁′ and R₆ are as defined above];

(13) the M₀ group:

a R₁(R₂S)C═N—R₆— group (wherein R₁, R₂ and R₆ are as defined above),

a R₂B(R₂′B′)C═N—R₆— group (wherein R₂ and R₆ are as defined above, R₂′ is the same as or different from R₂ and has the same meaning as that of R₂, and B and B′ are the same or different and represent an oxy group or a thio group),

a R₁R₁′N—(R₂S)C═N—R₆— group (wherein R₁, R₁′, R₂ and R₆ are as defined above),

a R₁N═C(SR₂)—NR₂′—R₆— group (wherein R₁, R₂, R₂′ and R₆ are as defined above) or

a R₁(R₁′O)N—R₆— group (wherein R₁, R₁′ and R₆ are as defined above);

(14) the N₀ group: a A₁₁-P(═O)(OR₁′)—R₄— group

wherein A₁₁ represents a R₁— group (wherein R₁ is as defined above), a R₁₀—R₆— group (wherein R₁ and R₆ are as defined above) or a R₁OCO—CHR₀— group (wherein R₁ and R₀ are as defined above), and R₁′ and R₄ are as defined above;

III. in (Y_(A0))_(q), Y_(A0) is a substituent on a carbon atom and represents a group included in the following X₀ group and Y₀ group, q represents 0, 1, 2, 3 or 4, the sum of p (wherein p is as defined above) and q is 5 or less, Y_(A0)s are the same as or different when q is 2 or more, and the adjacent two same or different Y_(A0)s may form a group included in the Z₀ group to be fused to the A ring when q is 2 or more;

(1) the X₀ group: a M_(a)- group, wherein M_(a) represents a R_(b)— group (wherein R_(b) represents a C1-C10 alkyl group optionally substituted with a halogen atom), a halogen atom, a nitro group, a cyano group, a hydroxy group, a R_(c)—B_(a)—R_(d)— group (wherein R_(c) represents a C1-C10 alkyl group optionally substituted with a halogen atom, B_(a) represents an oxy group, a thio group, a sulfinyl group or a sulfonyl group, and R_(d) represents a single bond or a C1-C10 alkylene group), a HOR_(d)— group (wherein R_(d) is as defined above), a R_(e)—CO—R_(d)— group (wherein R_(e) represents a hydrogen atom, or a C1-C10 alkyl group optionally substituted with a halogen atom, and R_(d) is as defined above), a R_(e)—CO—O—R_(d)— group (wherein R_(e) and R_(d) are as defined above), a R_(e)O—CO—R_(d)— group (wherein R_(e) and R_(d) are as defined above), a HO—CO—CH═CH— group, a R_(e)R_(e)′N—R_(d)— group (wherein R_(e) and R_(e)′ are the same or different, R_(e) is as defined above, R_(e)′ has the same meaning as R_(e) has, and R_(d) is as defined above), a R_(e)—CO—NR_(e)′—R_(d)— group (wherein R_(e), R_(e)′ and R_(d) are as defined above), a R_(b)O—CO—N(R_(e))—R_(d)— group (wherein R_(b), R_(e) and R_(d) are as defined above), a R_(e)R_(e)′N—CO—R_(d)— group (wherein R_(e), R_(e)′ and R_(d) are as defined above), a R_(e)R_(e)′N—CO—NR_(e)″—R_(d)— group (wherein R_(e), R_(e)′ and R_(e)″ are the same or different, R_(e) and R_(e)′ are as defined above, R_(e)″ has the same meaning as R_(e) has, and R_(d) is as defined above), a R_(e)R_(e)′N—C(═NR_(e)″)—NR_(e)′″—R_(d)— group (wherein R_(e), R_(e)′, R_(e)″ and R_(e)′″ are the same or different, R_(e), R_(e)′ and R_(e)″ are as defined above, R_(e)′″ has the same meaning as R_(e) has, and R_(d) is as defined above), a R_(b)—SO₂—NR_(e)—R_(d)— group (wherein R_(b), R_(e) and R_(d) are as defined above), a R_(e)R_(e)′N—SO₂—R_(d)— group (wherein R_(e), R_(e)′ and R_(d) are as defined above), a C2-C10 alkenyl group or a C2-C10 alkynyl group;

(2) the Y₀ group: a M_(b0)-R_(d)— group, wherein M_(b0) represents a M_(c0)- group

[wherein M_(c0) represents a M_(d0)-R_(d)′— group [wherein M_(d0) represents a 6 to 10-membered aryl group optionally substituted with a M_(a)- group (wherein M_(a) is as defined above), a 5 to 10-membered heteroaryl group optionally substituted with a M_(a)- group (wherein M_(a) is as defined above), a 3 to 10-membered cyclic hydrocarbon or heterocyclic group which is optionally substituted with a M_(a)- group (wherein M_(a) is as defined above) and which optionally contains an unsaturated bond, or a (b_(o))-group

(wherein (b_(o)) forms as defined above), a (c_(o))-group

(wherein (c₀) forms as defined above), a (d₀)-group

{wherein d_(o) forms a 5 to 12-membered hydrocarbon ring which is substituted with a carbonyl group or a thiocarbonyl group and further which may be optionally substituted with an oxy group, a thio group, a —NR₁— group (wherein R₁ is as defined above), a sulfinyl group or a sulfonyl group} or a (e₀)-group

{wherein e₀ forms a 5 to 12-membered hydrocarbon ring optionally substituted with a carbonyl group, a thiocarbonyl group, an oxy group, a thio group, a —NR₁— group (wherein R₁ is as defined above), a sulfinyl group or a sulfonyl group}, and R_(d)′ is the same as or different from R_(d) and has the same meaning as R_(d) has]],

a M_(c0)-B_(a)— group (wherein M_(c0) and B_(a) are as defined above), a M_(c0)-CO— group (wherein M_(c0) is as defined above), a M_(c0)-CO—O— group (wherein M_(c0) is as defined above), a M_(c0)O—CO— group (wherein M_(c0) is as defined above), a M_(c0)R_(e)N— group (wherein M_(c0) and R_(e) are as defined above), a M_(c0)-CO—NR_(e)— group (wherein M_(c0) and R_(e) are as defined above), a M_(c0)O—CO—NR_(e)— group (wherein M_(c0) and R_(e) are as defined above), a M_(c0)R_(e)N—CO— group (wherein M_(c0) and R_(e) are as defined above), a M_(c0)R_(e)N—CO—NR_(e)′— group (wherein M_(c0), R_(e) and R_(e)′ are as defined above), a M_(c0)R_(e)N—C(═NR_(e)′)—NR_(e)″— group (wherein M_(c0), R_(e), R_(e)′ and R_(e)″ are as defined above), a M_(c0)-SO₂—NR_(e)— group (wherein M_(c0) and R_(e) are as defined above) or a M_(c0)R_(e)N—SO₂— group (wherein M_(c0) and R_(e) are as defined above), and

R_(d) is as defined above;

(3) the Z₀ group: a 5 to 12-membered cyclic hydrocarbon or heterocyclic ring optionally substituted with a halogen atom, a C1-C10 alkoxy group, a C3-C10 alkenyloxy group, a C3-C10 alkynyloxy group, a carbonyl group, a thiocarbonyl group, an oxy group, a thio group, a sulfinyl group or a sulfonyl group, which is an aromatic or nonaromatic and monocyclic or fused ring and which is fused to the A ring;

IV. Q_(A0) represents a hydroxyl group, a (b₀)-group (wherein (b₀) is as defined above), an A₉-B₆—B_(c)— group [wherein A₉ and B₆ are as defined above, and B_(c) represent an oxy group or a —N((O)_(m)R₁)— group (wherein m and R₁ are as defined above), provided that B_(c) is not a sulfonyl group when A₉ is a hydrogen atom], an A₇″-SO₂—B_(c)— group (wherein A₇″ and B_(c) are as defined above), an A₈-SO₂—B_(c)— group (wherein A₈ and B_(c) are as defined above, provided that A₈ is not a hydrogen atom), a R₁R₁′N—SO₂—B_(c)— group (wherein R₁, R₁′ and B_(c) are as defined above), a (b₀)-SO₂—B_(c)— group (wherein (b₀) and B_(c) are as defined above), an A₉′-B_(c)— group (wherein A₉′ and B_(c) are as defined above), a D₅-R₄—B_(c)— group (wherein D₅, R₄ and B_(c) are as defined above), a M_(c0)-B₃—B_(c)— group (wherein M_(c0), B₃ and B_(c) are as defined above) or a M_(c0)-B_(c)— group (wherein M_(c0) and B_(c) are as defined above); V. T_(A0) represents a hydrogen atom, an A₀′-group (wherein A₉′ is as defined above), a D₅-R₄— group (wherein D₅ and R₄ are as defined above) or a M_(c0)- group (wherein M_(c0) is as defined above); and VI. K_(A0) represents a hydrogen atom, a halogen atom or a C1-C10 alkyl group, L_(A0) represents a hydrogen atom, a C1-C10 alkyl group or a M_(b0)- group (wherein M_(b0) is as defined above), or K_(A0) and L_(A0) together may form a C1-C10 alkylene group, or a C1-C10 alkenylene group optionally substituted with a M_(a) group or M_(a) groups which are the same or different; and

the term “as defined above” used for the same symbols among plural substituents means that the plural substituents independently represent the same meaning as that described above and, among the plural substituents, although the selection range of substituents to be selected is the same, selected substituents may be the same or different as long as they are selected within the range;

3. A cinnamoyl compound represented by the formula (III):

wherein, I. A represents a benzene ring or a pyridine ring; II. in (X_(A))_(p), X_(A) is a substituent on a carbon atom and represents a group included in any group or the following A to N groups, p represents 1, 2, 3, 4 or 5, and, X_(A)s are the same or different when p is 2 or more,

(1) the A group:

a D₁-R₄— group, wherein D₁ represents a (R₁—(O)_(k)-(A₁N—(O)_(k)′— group [wherein R₁ represents a hydrogen atom, or a C1-C10 alkyl group, or a C2-C10 alkyl group substituted with a halogen atom or a R₂—B₁— group (wherein R₂ represents a C1-C10 alkyl group, a C3-C10 alkenyl group or a C3-C10 alkynyl group, and B₁ represents an oxy group, a thio group, a sulfinyl group or a sulfonyl group), or a C3-C10 alkenyl group, or a C3-C10 alkynyl group, k represents 0 or 1, A₁ represents a R₃—(CHRO)_(m)—(B₂—B₃)_(m′)— group {wherein R₃ represents a hydrogen atom, or a C1-C10 alkyl group optionally substituted with a halogen atom or a R₂—B₁— group (wherein R₂ and B₁ are as defined above), or a C2-C10 alkenyl group, or a C2-C10 alkynyl group, R₀ represents a hydrogen atom, a C1-C10 alkyl group or a C2-C10 haloalkyl group, m represents 0 or 1, B₂ represents a single bond, an oxy group, a thio group or a —N((O)_(n)R₁′)— group (wherein R₁′ is the same as or different from R₁ and has the same meaning as R₁ has, and n represents 0 or 1), B₃ represents a carbonyl group, a thiocarbonyl group or a sulfonyl group, m′ represents 0 or 1, and when B₃ is a sulfonyl group, it does not occur that m is 0 and R₃ is a hydrogen atom at the same time}, and k′ represents 0 or 1], and R₄ represents a C1-C10 alkylene group, provided that a R₀′R₀″N—R₄— group (wherein R₀′ and R₀″ are the same as or different from R₀ and has the same meaning as R₀ has, and R₄ is as defined above) is excluded,

a D₂-R₄— group, wherein D₂ represents a cyano group, a R₁R₁′NC(′N—(O)_(n)-A₁)-group (wherein R₁, R₁′, n and A₁ are as defined above), an A₁N═C(—OR₂)— group (wherein A₁ and R₂ are as defined above) or a NH₂—CS— group, and R₄ is as defined above,

a D₃-R₄— group, wherein D₃ represents a nitro group or a R₁OSO₂— group (wherein R₁ is as defined above), and R₄ is as defined above, or

a R₁OSO₂— group, wherein R₁ is as defined above;

(2) the B group: an (a)-group

in (a), E₁ and E₁′ represent a methylene group optionally substituted with a C1-C10 alkyl group or a C1-C10 alkoxy group, or a carbonyl group, provided that E₁ and E₁′ are not a carbonyl group at the same time, E₂ represents a C2-C10 alkylene group optionally substituted with an oxy group, a thio group, a sulfinyl group, a sulfonyl group or a —NR₁′— group (wherein R₁′ is as defined above), or a C3-C10 alkenylene group optionally substituted with an oxy group, a thio group, a sulfinyl group, a sulfonyl group or a —NR₁′— group (wherein R₁′ is as defined above), and R₁ is as defined above;

(3) the C group: a C2-C10 alkenyl group substituted with a halogen atom, a R₂—B₁— group (wherein R₂ and B₁ are as defined above), a D₄-R₄— group [wherein D₄ represents a hydroxyl group or an A₁-O— group (wherein A₁ is as defined above), and R₄ is as defined above], a D₅- group [wherein D₅ represents an O═C(R₃)— group (wherein R₃ is as defined above), an A₁-(O)_(n)—N═C(R₃)— group (wherein A₁, n and R₃ are as defined above), a R₁—B₀—CO—R₄—(O)_(n)—N═C(R₃)— group (wherein R₁, R₄, n and R₃ are as defined above, and B₀ represents an oxy group, a thio group or a —N((O)_(m)R₁′)— group (wherein R₁′ and m are as defined above)}, a D₂-R₄—(O)_(n)—N═C(R₃)— group (wherein D₂, R₄, n and R₃ are as defined above) or a R₁A₁N—N═C(R₃)— group (wherein R₁, A₁ and R₃ are as defined above)], a R₁A₁N—O—R₄— group (wherein R₁, A₁ and R₄ are as defined above), a R₁ (A₁-(O)_(n)—)N— group (wherein R₁, A₁ and n are as defined above), a D₂- group (wherein D₂ is as defined above) or a D₃- group (wherein D₃ is as defined above);

(4) the D group: a C2-C10 alkynyl group substituted with a (b)-R₄— group [wherein, in (b)

G₁, G₂, G₄ and G₅ represent a methylene group which is connected with the adjacent atom via a single bond and which may be optionally substituted with a methyl group, or a methine group which is connected with the adjacent atom via a double bond and which may be optionally substituted with a methyl group, and G₃ represents a single bond, a double bond, a C1-C10 alkylene group optionally substituted with a methyl group, an oxy group, a thio group, a sulfinyl group, a sulfonyl group or a —NR₁— group (wherein R₁ is as defined above), or a C2-C10 alkenylene group optionally substituted with a methyl group, an oxy group, a thio group, a sulfinyl group, a sulfonyl group or a —NR₁— group (wherein R₁ is as defined above); and R₄ is as defined above], a (c)-R₄— group (wherein, in (c)

J₁, J₂ and J₃ are the same or different, and represent a methine group optionally substituted with a methyl group, or a nitrogen atom; and R₄ is as defined above), a halogen atom, a R₂—B₁—R₄— group (wherein R₂, B₁ and R₄ are as defined above), a D₄-R₄— group (wherein D₄ and R₄ are as defined above), a D₅- group (wherein D₅ is as defined above), a D₁-R₄— group (wherein D₁ and R₄ are as defined above), a D₂- group (wherein D₂ is as defined above) or a D₃-R₄— group (wherein D₃ and R₄ are as defined above);

(5) the E group: an A₂-CO—R₅— group, provided that R₅ is not a vinylene group when A₂ is a hydroxyl group, wherein A₂ represents

(i) an A₃-B₄— group

wherein A₃ represents a hydrogen atom, or a C1-C10 alkyl group, or a C2-C10 haloalkyl group, or a C2-C10 alkenyl group optionally substituted with a halogen atom, or a C3-C10 alkynyl group optionally substituted with a halogen atom, or R_(a)—(R₄)_(m)— group (wherein R_(a) represents a phenyl group, a pyridyl group, a furyl group or a thienyl group, which may be optionally substituted with a halogen atom, a C1-C10 alkyl group, a C1-C10 alkoxy group or a nitro group, and R₄ and m are as defined above), or a C1-C10 alkyl group substituted with a (b)-R₄— group (wherein (b) and R₄ are as defined above), a (c)-R₄— group (wherein (c) and R₄ are as defined above), a R₂—B₁—R₄— group (wherein R₂, B₁ and R₄ are as defined above), a D₄-R₄— group (wherein D₄ and R₄ are as defined above), a D₅- group (wherein D₅ is as defined above), a D₁-R₄— group (wherein D₁ and R₄ are as defined above), a D₂- group (wherein D₂ is as defined above), a D₃-R₄— group (wherein D₃ and R₄ are as defined above) or an A₄-SO₂—R₄— group {wherein A₄ represents a (b)-group (wherein (b) is as defined above), a (c)-group (wherein (c) is as defined above) or a R₁R₁′N— group (wherein R₁ and R₁′ are as defined above), and R₄ is as defined above}, and

B₄ represents an oxy group, a thio group or a —N((O)_(m)R₁)— group (wherein R₁ and m are as defined above), provided that A₃ is not a hydrogen atom when B₄ is a thio group,

(ii) a R₁—B₄—CO—R₄—B₄′— group

wherein R₁, B₄ and R₄ are as defined above, B₄′ is the same as or different from B₄ and has the same meaning as B₄ has, provided that R₂ is not a hydrogen atom when B₄ is a thio group, or

a D₂-R₄—B₄— group, wherein D₂, R₄ and B₄ are as defined above,

(iii) a R₂—SO₂—NR₁— group

wherein R₂ is as defined above, provided that a hydrogen atom is excluded; and R₁ is as defined above,

(iv) a (b)-group, wherein (b) is as defined above,

(v) a (c)-group, wherein (c) is as defined above, or

(vi) a R₁A₁N—NR₁′— group, wherein R₁, A₁ and R₁′ are as defined above, and

R₅ represents a C2-C10 alkenylene group optionally substituted with a halogen atom, or a C2-C10 alkynylene group;

(6) the F group: an A₅-B₅—R₆— group

wherein A₅ represents a C2-C10 alkyl group substituted with a D₄- group (wherein D₄ is as defined above), a D₁- group (wherein D₁ is as defined above), a D₃- group (wherein D₃ is as defined above) or an A₄-SO₂— group (wherein A₄ is as defined above), or a C1-C10 alkyl group substituted with a R₂—B₁— group (wherein R₂ and B₁ are as defined above), a D₂- group (wherein D₂ is as defined above), a D₅- group (wherein D₅ is as defined above) or an A₂-CO— group (wherein A₂ is as defined above), B₅ represents a B₁— group (wherein B₁ is as defined above) or a —NA₁- group (wherein A₁ is as defined above), and R₆ represents a single bond or a C1-C10 alkylene group;

(7) the G group: an A₆-B₅—R₆— group

wherein A₆ represents an (a)-R₄— group (wherein (a) and R₄ are as defined above), or a C2-C10 alkenyl group, or a C2-C10 alkynyl group, or a C2-C10 alkenyl group substituted with a halogen atom, a R₂—B₁— group (wherein R₂ and B₁ are as defined above), a D₅- group (wherein D₅ is as defined above), a D₂- group (wherein D₂ is as defined above) or an A₂-CO— group (wherein A₂ is as define above), or a C2-C10 alkynyl group substituted with a halogen atom, a R₂—B₁— group (wherein R₂ and B₁ are as defined above), a D₅- group (wherein D₅ is as defined above), a D₂- group (wherein D₂ is as defined above) or an A₂-CO— group (wherein A₂ is as defined above), or a C3-C10 alkenyl group substituted with a (b)-group (wherein (b) is as defined above), a (c)-group (wherein (c) is as defined above), a D₄- group (wherein D₄ is as defined above), a D₁- group (wherein D₁ is as defined above) or a D₃- group (wherein D₃ is as defined above), or a C3-C10 alkynyl group substituted with a D₄- group (wherein D₄ is as defined above), a D₁- group (wherein D₁ is as defined above) or a D₃- group (wherein D₃ is as defined above), and B₅ and R₆ are as defined above;

(8) the H group:

a D₂-N(—(O)_(n)-A₁)-R₆— group (wherein D₂, n, A₁ and R₆ are as defined above),

a D₂- group (wherein D₂ is as defined above, provided that a cyano group is excluded),

a R₁ (R₁′ (O)_(n))N—CR₁″═N—R₆— group (wherein R₁, R₁′, n and R₆ are as defined above, R¹″ is the same as or different from R₁ and has the same meaning as R₁ has),

a R₁—(O)_(n)—N═CR₁′—NR₂—R₆— group (wherein R₁, n, R₁′, R₂ and R₆ are as defined above),

a R₂—B₃—NR₁—CO—NR₁′—R₆— group (wherein R₂, B₃, R₁, R₁′ and R₆ are as defined above),

a D₂-CO—NR₁—R₆— group (wherein D₂, R₁ and R₆ are as defined above) or

an A₂-COCO—NR₁—R₆— group (wherein A₂, R₁ and R₆ are as defined above);

(9) the I group:

an A₇-B₆—N((O)_(n)R₁)—R₆— group [wherein A₇ represents a C2-C10 alkenyl group optionally substituted with a halogen atom, or a C2-C10 alkynyl group, or a C3-C10 haloalkynyl group, or a R₂—B₁—R₄— group (wherein R₂, B₁ and R₄ are as defined above), or a D₄-R₄— group (wherein D₄ and R₄ are as defined above), or a D₅-R₄— group (wherein D₅ and R₄ are as defined above), or a D₁-R₄— group (wherein D₁ and R₄ are as defined above), or a (b)-R₄— group (wherein (b) and R₄ are as defined above), or a (c)-R₄— group (wherein (c) and R₄ are as defined above), or a D₃-R₄— group (wherein D₃ and R₄ are as defined above), or an A₄-SO₂—R₄— group (wherein A₄ and R₄ are as defined above), or an A₂-CO—R₄— group (wherein A₂ and R₄ are as defined above), B₆ represents a carbonyl group or a thiocarbonyl group, and n, R₁ and R₆ are as defined above],

an A₈-CS—N((O)_(n)R₁)—R₆— group [wherein A₈ represents a hydrogen atom or a C1-C10 alkyl group optionally substituted with a halogen atom, and n, R₁ and R₆ are as defined above],

an A₇′—B₂′—B₃—N((O)_(n)R₁)—R₆— group [wherein A₇′ represents a C3-C10 alkenyl group optionally substituted with a halogen atom, or a C3-C10 alkynyl group optionally substituted with a halogen atom, or a R₂—B₁—R₄′— group (wherein R₂ and B₁ are as defined above, and R₄′ represents a C2-C10 alkylene group), or a D₄-R₄′— group (wherein D₄ and R₄′ are as defined above), or a D₁-R₄′— group (wherein D₁ and R₄′ are as defined above), or a (b)-R₄′— group (wherein (b) and R₄′ are as defined above), or a (c)-R₄′— group (wherein (c) and R₄′ are as defined above), or a D₂-R₄— group (wherein D₂ and R₄ are as defined above), or a D₃-R₄′— group (wherein D₃ and R₄′ are as defined above), or an A₂-CO—R₄— group (wherein A₂ and R₄ are as defined above), B₂′ represents an oxy group, a thio group or a —N((O)_(n′)R₁′)— group (wherein n′ is the same as or different from n and has the same meaning as n has, and R₁′ is as defined above), and B₃, n, R₁ and R₆ are as defined above],

an A₈′-B₂′—CS—N((O)_(n)R₁)—R₄— group [wherein A₈′ represents a C1-C10 alkyl group or a C2-C10 haloalkyl group, B₂′ is as defined above, and n, R₁ and R₆ are as defined above],

an A₈′-S—B₃′—N((O)_(n)R₁)—R₆— group [wherein A₈′, n, R₁ and R₆ are as defined above, and B₃′ represents a carbonyl group or a sulfonyl group] or

an A₇″-SO₂—N((O)_(n)R₁)—R₆— group [wherein A₇″ represents a C2-C10 alkenyl group, or a C3-C10 alkenyl group substituted with a halogen atom, or a C3-C10 alkynyl group optionally substituted with a halogen atom, or a R₂—B₁—R₄′— group (wherein R₂, B₁ and R₄′ are as defined above), or a D₄-R₄′— group (wherein D₄ and R₄′ are as defined above), or a D₅-R₄— group (wherein D₅ and R₄ are as defined above), or a D₁-R₄′— group (wherein D₁ and R₄′ are as defined above), or a (b)-R₄′— group (wherein (b) and R₄′ are as defined above), or a (c)-R₄′— group (wherein (c) and R₄′ are as defined above), or a D₂-R₄— group (wherein D₂ and R₄ are as defined above), or a NO₂—R₄— group (wherein R₄ is as defined above), or an A₂-CO—R₄— group (wherein A₂ and R₄ are as defined above), and n, R₁ and R₄ are as defined above];

(10) the J group:

an A₇-CO— group (wherein A₇ is as defined above),

an A₉-CS— group (wherein A₉ represents A₇ or A₈),

an A₉′(O)_(m)N═C(A₉)-group (wherein A₉′ represents A₇′ or A₈′, and m and A₉ are as defined above),

a D₂-CO— group (wherein D₂ is as defined above),

an A₂-COCO— group (wherein A₂ is as defined above),

an A₉-CO—B₁—R₆— group (wherein A₉ and R₆ are as defined above, and B₁′ represents an oxy group or a thio group, provided that A₉ is not A8 when B₁′ is an oxy group),

an A₉-CS—B₁′—R₆— group (wherein A₉, B₁′ and R₆ are as defined above),

an A₇ ¹″-SO₂—B₁′—R₆— group (wherein A₇″, B₁′ and R₆ are as defined above),

an A₈-SO₂—B₁′—R₆— group (wherein A₈, B₁′ and R₆ are as defined above, provided that A₈ is not a hydrogen atom),

an A₉′-B₂′—B₃—B₁′—R₆— group (wherein A₉′, B₂′, B₃, B₁′ and R₆ are as defined above), or

a C2-C10 alkenyl group substituted with a (b)-group (wherein (b) is as defined above) or a (c)-group (wherein (c) is as defined above);

(11) the K group: an A₁₀-N((O)_(n)R)—CO—R₆— group

wherein A₁₀ represents a hydrogen atom (provided that n is not 0), an A₇″-SO₂— group (wherein A₇″ is as defined above), an A₈-SO₂— group (wherein A₈ is as defined above, provided that A₈ is not a hydrogen atom), an A₉′O— group (wherein A₉′ is as defined above, provided that n is not 1), an A₉′-group (wherein A₉′ is as defined above, provided that A₈′ is excluded when n is 0), a R₂OCH₂— group (wherein R₂ is as defined above), an A₂-CO—R₄— group (wherein A₂ and R₄ are as defined above) or an A₂-CO—CH(CH₂CO-A₂)-group (wherein A₂ is as defined above), and n, R₁ and R₆ are as defined above;

(12) the L group:

an A₁₀′-N((O)_(n)R₁)—SO₂—R₆— group [wherein A₁₀ represents a hydrogen atom (provided that n is not 0), an A₉′O— group (wherein A₉′ is as defined above, provided that n is not 1), an A₉′-group (wherein A₉′ is as defined above, provided that A₈′ is excluded when n is 0), a R₂—CO— group (wherein R₂ is as defined above), an A₂-CO—R₄— group (wherein A₂ and R₄ are as defined above) or an A₂-CO—CH(CH₂CO-A₂)-group (wherein A₂ is as defined above), and n, R₁ and R₆ are as defined above],

an A₉″R₁N—SO₂—N((O)_(n)R₁′)—R₆— group [wherein A₉″ represents a hydrogen atom or an A₉′-group (wherein A₉′ is as defined above), and R₁, n, R₁′ and R₆ are as defined above] or

a (b)-SO₂—N((O)_(n)R₁′)—R₆— group [wherein (b), n, R₁′ and R₆ are as defined above];

(13) the M group:

a R₁(R₂S)C═N—R₆— group (wherein R₁, R₂ and R₆ are as defined above),

a R₂B(R₂′B′)C═N—R₆— group (wherein R₂ and R₆ are as defined above, R₂′ is the same as or different from R₂ and has the same meaning as R₂ has, and B and B′ are the same or different and represent an oxy group or a thio group),

a R₁R₁′N—(R₂S)C═N—R₆— group (wherein R₁, R₁′, R₂ and R₆ are as defined above),

a R₁N═C(SR₂)—NR₂′—R₆— group (wherein R₁, R₂, R₂′ and R₆ are as defined above) or

a R₁(R₁′O)N—R₆— group (wherein R₁, R₁′ and R₆ are as defined above);

(14) the N group: an A₁₁-P(═O)(OR₁′)—R₄— group

wherein A₁₁ represents a R₁— group (wherein R₁ is as defined above), a R₁O—R₆— group (wherein R₁ and R₆ are as defined above) or a R₁OCO—CHR₀— group (wherein R₁ and R₀ are as defined above), and R₁′ and R₄ are as defined above;

III. in (Y_(A))_(q), Y_(A) is a substituent on a carbon atom and represents a group included in the following X group or Y group, q represents 0, 1, 2, 3 or 4, the sum of p (wherein p is as defined above) and q is 5 or less, Y_(A)s are the same or different when q is 2 or more, and the adjacent two same or different Y_(A)s together may form a group included in the Z group to be fused to the A ring when q is 2 or more,

(1) the X group: a M_(a)- group

wherein M_(a) represents a R_(b)— group (wherein R_(b) represents a C1-C10 alkyl group optionally substituted with a halogen atom), a halogen atom, a nitro group, a cyano group, a R_(c)—B_(a)—R_(d)— group (wherein R_(c) represents a C1-C10 alkyl group optionally substituted with a halogen atom, B_(a) represents an oxy group, a thio group, a sulfinyl group or a sulfonyl group, and R_(d) represents a single bond or a C1-C10 alkylene group), a HOR_(d)— group (wherein R_(d) is as defined above), a R_(e)—CO—R_(d)— group (wherein R_(e) represents a hydrogen atom, or a C1-C10 alkyl group optionally substituted with a halogen atom, and R_(d) is as defined above), a R_(e)—CO—O—R_(d)— group (wherein R_(e) and R_(d) are as defined above), a R_(e)O—CO—R_(d)— group (wherein R_(e) and R_(d) are as defined above), a HO—CO—CH═CH— group, a R_(e)R_(e)′N—R_(d)— group (wherein R_(e) and R_(e)′ are the same or different, R_(e) is as defined above, R_(e)′ has the same meaning as R_(e) has, and R_(d) is as defined above), a R_(e)—CO—NR_(e)′—R_(d)— group (wherein R_(e), R_(e)′ and R_(d) are as defined above), a R_(b)O—CO—N(R_(e))—R_(d)— group (wherein R_(b), R_(e) and R_(d) are as defined above), a R_(e)R_(e)′N—CO—R_(d)— group (wherein R_(e), R_(e)′ and R_(d) are as defined above), a R_(e)R_(e)′N—CO—NR_(e)″—R_(d)— group (wherein R_(e), R_(e)′ and R_(e)″ are the same or different, R_(e) and R_(e)′ are as defined above, R_(e)″ has the same meaning as R_(e) has, and R_(d) is as defined above), a R_(e)R_(e)′N—C(═NR_(e)″)—NR_(e)′″—R_(d)— group (wherein R_(e), R_(e)′, R_(e)″ and R_(e)′″ are the same or different, R_(e), R_(e)′ and R_(e)″ are as defined above, R_(e)′″ has the same meaning as R_(e) has, and R_(d) is as defined above), a R_(b)—SO₂—NR_(e)—R_(d)— group (wherein R_(b), R_(e) and R_(d) are as defined above), a R_(e)R_(e)′N—SO₂—R_(d)— group (wherein R_(e), R_(e)′ and R_(d) are as defined above), a C2-C10 alkenyl group or a C2-C10 alkynyl group;

(2) the Y group: a M_(b)-R_(d)— group, wherein M_(b) represents a M_(c)- group

[wherein M_(c) represents a M_(d)-R_(d)′— group [wherein M_(d) represents a phenyl group optionally substituted with a M_(a)- group (wherein M_(a) is as defined above), a pyridyl group optionally substituted with a M_(a)- group (wherein M_(a) is as defined above), a naphthyl group optionally substituted with a M_(a)- group (wherein M_(a) is as defined above), a (b)-group (wherein (b) is as defined above), a (c)-group (wherein (c) is as defined above), a (d)-group

(wherein 1 is 2, 3 or 4, B_(b) represents an oxy group or a thio group) or an (e)-group

(wherein 1 and B_(b) are as defined above), and R_(d)′ is the same as or different from R_(d) and has the same meaning as R_(d) has]], a M_(c)-B_(a)' group (wherein M_(c) and B_(a) are as defined above), a M_(c)-CO— group (wherein M_(c) is as defined above), a M_(c)-CO—O— group (wherein M_(c) is as defined above), a M_(c)O—CO— group (wherein M_(c) is as defined above), a M_(c)R_(e)N— group (wherein M_(c) and R_(e) are as defined above), a M_(c)-CO—NR_(e)— group (wherein M_(c) and R_(e) are as defined above), a M_(c)O—CO—NR_(e)— group (wherein M_(c) and R_(e) are as defined above), a M_(c)R_(e)N—CO— group (wherein M_(c) and R_(e) are as defined above), a M_(c)R_(e)N—CO—NR_(e)′— group (wherein M_(c), R_(e) and R_(e)′ are as defined above), a M_(c)R_(e)N—C(═NR_(e)′)—NR_(e)″— group (wherein M_(c), R_(e), R_(e)′ and R_(e)″ are as defined above), a M_(c)-SO₂—NR_(e)— group (wherein M_(c) and R_(e) are as defined above) or a M_(c)R_(e)N—SO₂— group (wherein M_(c) and R_(e) are as defined above), and R_(d) is as defined above;

(3) the Z group:

a —N═C(Y_(a))—Y_(a)′— group (wherein Y_(a) represents a hydrogen atom, or a C1-C10 alkyl group optionally substituted with a halogen atom, or a C1-C10 alkoxy group, and Y_(a)′ represents an oxy group, a thio group, or an imino group optionally substituted with a C1-C10 alkyl group),

a —Y_(b)—Y_(b)—Y_(b)″— group (wherein Y_(b) and Y_(b)″ are the same or different, and represent a methylene group, an oxy group, a thio group, a sulfinyl group, or an imino group optionally substituted with a C1-C10 alkyl group, and Y_(b)′ represents a C1-C4 alkylene group optionally substituted with a halogen atom, or a C1-C4 alkylene group optionally having an oxo group) or

a —Y_(c)—O—Y_(c)′—O— group (wherein Y_(c) and Y_(c)′ are the same or different, and represent a C1-C10 alkylene group);

IV. Q_(A) represents a hydroxyl group, a (b)-group (wherein (b) is as defined above), an A₉-B₆—B_(c)— group [wherein A₉ and B₆ are as defined above, and B_(c) represents an oxy group or a —N((O)_(m)R₁)— group (wherein m and R₁ are as defined above), provided that B_(c) is not a sulfonyl group when A₉ is a hydrogen atom], an A₇″-SO₂—B_(c)— group (wherein A₇″ and B_(c) are as defined above), an A₈-SO₂—B_(c)— group (wherein A₈ and B_(c) are as defined above, provided that A₈ is not a hydrogen atom), a R₁R₁′N—SO₂—B_(c)— group (wherein R₁, R₁′ and B_(c) are as defined above), a (b)-SO₂—B_(c)— group (wherein (b) and B_(c) are as defined above), an A₉′-B_(c)— group (wherein A₉′ and B_(c) are as defined above), a D₅-R_(c)—B_(c)— group (wherein D₅, R₄ and B_(c) are as defined above), a M_(c)-B₃—B_(c)— group (wherein M_(c), B₃ and B_(c) are as defined above) or a M_(c)-B_(c)— group (wherein M_(c) and B_(c) are as defined above); V. T_(A) represents a hydrogen atom, an A₉′-group (wherein A₉′ is as defined above), a D₅-R₄— group (wherein D₅ and R₄ are as defined above) or a M_(c)- group (wherein M_(c) is as defined above); and VI. K_(A) represents a hydrogen atom, a halogen atom or a C1-C10 alkyl group, and L_(A) represents a hydrogen atom, a C1-C10 alkyl group or a M_(b)- group (wherein M_(b) is as defined above), or K_(A) and L_(A) together may form a C1-C10 alkylene group or a —C(M_(a)′)=C(M_(a)″)-C(M_(a)′″)=C(M_(a)″″)-group (wherein M_(a)′, M_(a)″, M_(a)′″ and M_(a)″″ are the same or different, and the same as or different from M_(a), and represent a hydrogen atom or M_(a));

the term “as defined above” used for the same symbols among plural substituents means that the plural substituents independently represent the same meaning as that described above and, among the plural substituents, although the selection range of substituents to be selected is the same, selected substituents may be the same or different as long as they are selected within the range;

4. A cinnamoyl compound represented by the formula (IV):

wherein

A represents a benzene ring or a pyridine ring,

X_(a) is a substituent on a carbon atom, and represents a C1-C10 alkyl group substituted with a cyano group; a C1-C10 alkyl group substituted with a tetrahydropyran-4-ylidene group; a C2-C10 alkenyl group substituted with a halogen atom or a cyano group; a C2-C10 alkenyl group substituted with a C1-C10 alkoxycarbonyl group; a C3-C10 alkynyl group substituted with a hydroxyl group; an a₀-r₁-b-r₁′-group {wherein a₀ represents a methyl group substituted with a C1-C10 alkylthio group, a methyl group substituted with a C1-C10 alkylsulfinyl group, a methyl group substituted with a C1-C10 alkylsulfonyl group, a C2-C10 alkenyl group, a C2-C10 alkynyl group, a r₂O—CO— group (wherein r₂ represents a C1-C10 alkyl group, or a C2-C10 alkyl group substituted with a hydroxyl group), a carboxyl group, a rr′N—CO— group (wherein r and r′ are the same or different, and represent a hydrogen atom or a C1-C10 alkyl group), an a₁-NH—CO— group (wherein a₁ represents a C2-C10 alkyl group substituted with a C1-C10 alkoxy group), an a₁′-CO— group (wherein a₁′ represents a morpholino group), a rr′N—CH₂— group (wherein r and r′ are as defined above), a r₀-(O)₁—CONH—CH₂— group (wherein r₀ represents a C1-C10 alkyl group, and 1 represents 0 or 1), a r-OCH₂— group (wherein r is as defined above), a r₀-CO— group (wherein r₀ is as defined above), a cyano group, or a sulfomethyl group, r₁ represents a C1-C10 alkylene group, r₁′ represents a single bond or a C1-C10 alkylene group, and b represents an oxy group, a thio group, a sulfinyl group, a sulfonyl group or a imino group}; an a₂-y-CO—NH— group (wherein a₂ represents a C2-C10 alkyl group substituted with a C1-C10 alkoxy group, and y represents an oxy group or an imino group); a r₀O—COCO—NH— group (wherein r₀ is as defined above); an a₃-z-NH— group (wherein a₃ represents a C2-C10 alkenyl group, or a C1-C10 alkyl group substituted with a C1-10 alkoxy group, a C1-C10 alkoxycarbonyl group, a carboxy group or a cyano group, and z represents a carbonyl group or a sulfonyl group); an a₄-NHCO— group {wherein a₄ represents a C1-C10 alkoxy group, or a C3-C10 alkenyloxy group, or a r₀-SO₂— group (wherein r₀ is as defined above), or a C2-C10 alkyl group substituted with a hydroxyl group or a C1-C10 alkoxy group, or a C1-C10 alkyl group substituted with a rO—CO— group (wherein r is as defined above), a cyano group or an aminocarbonyl group, or a rO—CO—(rO—COCH₂)CH— group (wherein r is as defined above)}; an a₅-NHSO₂— group (wherein a₅ represents a C2-C10 alkyl group substituted with a C1-C10 alkoxy group); a r₀ON═CH— group (wherein r₀ is as defined above); a r₀NHCSNH— group (wherein r₀ is as defined above); a r₀NHC(—Sr₀′)═N— group (wherein r₀ is as defined above, r₀′ is the same as the different from r₀ and has the same meaning as r₀ has); or a (r₀O)₂P(═O)CH₂— group (wherein r₀ is as defined above);

p represents 1, 2 or 3, and when p is 2 or more, X_(a)s are the same or different;

Y_(a) represents a halogen atom, a nitro group, a r₀CO—NH— group (wherein r₀ is as defined above), a C1-C10 alkyl group or a C1-C10 alkoxy group;

q represents 0, 1 or 2, and when q is 2 or more, Y_(a)s are the same or different;

q_(a) represents a r_(a)-O— group {wherein r_(a) represents a hydrogen atom, a C1-C10 alkyl group, a C3-C10 alkenyl group, a C3-C10 alkynyl group, a C1-C10 alkyl group substituted with a r₀r₀′N—CH₂— group (wherein r₀ and r₀′ are as defined above), a rOCH₂— group (wherein r is as defined above), a r₀-CO— group (wherein r₀ is as defined above), a C1-C10 alkoxycarbonyl group, a carboxy group, an aminocarbonyl group or a cyano group, or a r₃-r₁- group (wherein r₃ represents a phenyl group or a pyridyl group, and r₁ is as defined above)); a piperidino group; a morpholino group; or a r₄r₄′N— group (wherein r₄ and r₄′ are the same or different, and represent a hydrogen atom, a C1-C10 alkyl group, a C3-C10 alkenyl group, a C3-C10 alkynyl group, or a C2-C10 alkyl group substituted with a C1-C10 alkoxy group, provided that r₄ and r₄′ are not a hydrogen atom at the same time);

t_(a) represents a r_(b)- group (wherein r_(b) is the same as or different from r_(a), and has the same meaning as r_(a) has) or a r₃′-group (wherein r₃′ is the same as or different from r₃, and has the same meaning as r₃ has);

K_(a) represents a hydrogen atom, a halogen atom or a C1-C10 alkyl group, and L_(a) represents a hydrogen atom or a C1-C10 alkyl group; or

K_(a) and L_(a) together may form a C1-C10 alkylene group or a 1,3-butadienylene group;

the term “as defined above” used for the same symbols among plural substituents means that the plural substituents independently represent the same meaning as that described above and, among the plural substituents, although the selection range of substituents to be selected is the same, selected substituents may be the same or different as long as they are selected within the range;

5. A cinnamoyl compound represented by the formula (V):

wherein

a represents a benzene ring or a pyridine ring;

x represents a methine group or a nitrogen atom;

X_(a) is a substituent on a carbon atom, and represents a C1-C10 alkyl group substituted with a cyano group; a C1-C10 alkyl group substituted with a tetrahydropyran-4-ylidene group; a C2-C10 alkenyl group substituted with a halogen atom or a cyano group; a C2-C10 alkenyl group substituted with a C1-C10 alkoxycarbonyl group; a C3-C10 alkynyl group substituted with a hydroxyl group; an a₀-r₁-b-r₁′-group {wherein a₀ represents a methyl group substituted with a C1-C10 alkylthio group, a methyl group substituted with a C1-C10 alkylsulfinyl group, a methyl group substituted with a C1-C10 alkylsulfonyl group, a C2-C10 alkenyl group, a C2-C10 alkynyl group, a r₂O—CO— group (wherein r₂ represents a C1-C10 alkyl group, or a C2-C10 alkyl group substituted with a hydroxyl group), a carboxyl group, a rr′N—CO— group (wherein r and r′ are the same or different, and represent a hydrogen atom or a C1-C10 alkyl group), an a₁-NH—CO— group (wherein a₁ represents a C2-C10 alkyl group substituted with a C1-C10 alkoxy group), an a₁′-CO— group (wherein a₁′ represents a morpholino group), a rr′N—CH₂— group (wherein r and r′ are as defined above), a r₀-(O)₁—CONH—CH₂— group (wherein r₀ represents a C1-C10 alkyl group, and 1 represents 0 or 1), a r-OCH₂— group (wherein r is as defined above), a r₀-CO— group (wherein r₀ is as defined above), a cyano group, or a sulfomethyl group, r₁ represents a C1-C10 alkylene group, r₁′ represents a single bond or a C1-C10 alkylene group, and b represents an oxy group, a thio group, a sulfinyl group, a sulfonyl group or a imino group); an a₂-y-CO—NH— group (wherein a₂ represents a C2-C10 alkyl group substituted with a C1-C10 alkoxy group, and y represents an oxy group or an imino group); a r₀O—COCO—NH— group (wherein r₀ is as defined above); an a₃-z-NH— group (wherein a₃ represents a C2-C10 alkenyl group, or a C1-C10 alkyl group substituted with a C1-10 alkoxy group, a C1-C10 alkoxycarbonyl group, a carboxy group or a cyano group, and z represents a carbonyl group or a sulfonyl group); an a₄-NHCO— group {wherein a₄ represents a C1-C10 alkoxy group, or a C3-C10 alkenyloxy group, or a r₀-SO₂— group (wherein r₀ is as defined above), or a C2-C10 alkyl group substituted with a hydroxyl group or a C1-C10 alkoxy group, or a C1-C10 alkyl group substituted with a rO—CO— group (wherein r is as defined above), a cyano group or an aminocarbonyl group, or a rO—CO—(rO—COCH₂)CH— group (wherein r is as defined above)}; an a₅-NHSO₂— group (wherein a₅ represents a C2-C10 alkyl group substituted with a C1-C10 alkoxy group); a r₀ON═CH— group (wherein r₀ is as defined above); a r₀NHCSNH— group (wherein r₀ is as defined above); a r₀NHC(—Sr₀′)═N— group (wherein r₀ is as defined above, r₀′ is the same as the different from r₀ and has the same meaning as r₀ has); or a (r₀O)₂P(═O)CH₂— group (wherein r₀ is as defined above);

p represents 1, 2 or 3, and when p is 2 or more, X_(a)s are the same or different;

Y_(a) represents a halogen atom, a nitro group, a r₀CO—NH— group (wherein r₀ is as defined above), a C1-C10 alkyl group or a C1-C10 alkoxy group;

q represents 0, 1 or 2, and when q is 2 or more, Y_(a)s are the same or different;

q_(a) represents a r_(a)-O— group (wherein r_(a) represents a hydrogen atom, a C1-C10 alkyl group, a C3-C10 alkenyl group, a C3-C10 alkynyl group, a C1-C10 alkyl group substituted with a r₀r₀′N—CH₂— group (wherein r₀ and r₀′ are as defined above), a rOCH₂— group (wherein r is as defined above), a r₀-CO— group (wherein r₀ is as defined above), a C1-C10 alkoxycarbonyl group, a carboxy group, an aminocarbonyl group or a cyano group, or a r₃-r₁- group (wherein r₃ represents a phenyl group or a pyridyl group, and r₁ is as defined above)}; a piperidino group; a morpholino group; or a r₄r₄′N— group (wherein r₄ and r₄′ are the same or different, and represent a hydrogen atom, a C1-C10 alkyl group, a C3-C10 alkenyl group, a C3-C10 alkynyl group, or a C2-C10 alkyl group substituted with a C1-C10 alkoxy group, provided that r₄ and r₄′ are not a hydrogen atom at the same time);

t_(a) represents a r_(b)- group (wherein r_(b) is the same as or different from r_(a), and has the same meaning as r_(a) has) or a r₃′-group (wherein r₃′ is the same as or different from r₃, and has the same meaning as r₃ has);

K_(a) represents a hydrogen atom, a halogen atom or a C1-C10 alkyl group, and L_(a) represents a hydrogen atom or a C1-C10 alkyl group; or

K_(a) and L_(a) together may form a C1-C10 alkylene group or a 1,3-butadienylene group;

the term “as defined above” used for the same symbols among plural substituents means that the plural substituents independently represent the same meaning as that described above and, among the plural substituents, although the selection range of substituents to be selected is the same, selected substituents may be the same or different as long as they are selected within the range;

6. A 2(1H)-pyridinone compound represented by the formula (VI):

wherein

a represents a benzene ring or a pyridine ring;

x represents a methine group or a nitrogen atom;

X_(a) is a substituent on a carbon atom, and represents a C1-C10 alkyl group substituted with a cyano group; a C1-C10 alkyl group substituted with a tetrahydropyran-4-ylidene group; a C2-C10 alkenyl group substituted with a halogen atom or a cyano group; a C2-C10 alkenyl group substituted with a C1-C10 alkoxycarbonyl group; a C3-C10 alkynyl group substituted with a hydroxyl group; an a₀-r₁-b-r₁′-group {wherein a₀ represents a methyl group substituted with a C1-C10 alkylthio group, a methyl group substituted with a C1-C10 alkylsulfinyl group, a methyl group substituted with a C1-C10 alkylsulfonyl group, a C2-C10 alkenyl group, a C2-C10 alkynyl group, a r₂O—CO— group (wherein r₂ represents a C1-C10 alkyl group, or a C2-C10 alkyl group substituted with a hydroxyl group), a carboxyl group, a rr′N—CO— group (wherein r and r′ are the same or different, and represent a hydrogen atom or a C1-C10 alkyl group), an a₁-NH—CO— group (wherein a₁ represents a C2-C10 alkyl group substituted with a C1-C10 alkoxy group), an a₁′-CO— group (wherein a₁′ represents a morpholino group), a rr′N—CH₂— group (wherein r and r′ are as defined above), a r₀-(O)₁—CONH—CH₂— group (wherein r₀ represents a C1-C10 alkyl group, and 1 represents 0 or 1), a r-OCH₂— group (wherein r is as defined above), a r₀-CO— group (wherein r₀ is as defined above), a cyano group, or a sulfomethyl group, r₁ represents a C1-C10 alkylene group, r₁′ represents a single bond or a C1-C10 alkylene group, and b represents an oxy group, a thio group, a sulfinyl group, a sulfonyl group or a imino group}; an a₂-y-CO—NH— group (wherein a₂ represents a C2-C10 alkyl group substituted with a C1-C10 alkoxy group, and y represents an oxy group or an imino group); a r₀O—COCO—NH— group (wherein r₀ is as defined above); an a₃-z-NH— group (wherein a₃ represents a C2-C10 alkenyl group, or a C1-C10 alkyl group substituted with a C1-10 alkoxy group, a C1-C10 alkoxycarbonyl group, a carboxy group or a cyano group, and z represents a carbonyl group or a sulfonyl group); an a₄-NHCO— group {wherein a₄ represents a C₁-C10 alkoxy group, or a C3-C10 alkenyloxy group, or a r₀-SO₂— group (wherein r₀ is as defined above), or a C2-C10 alkyl group substituted with a hydroxyl group or a C1-C10 alkoxy group, or a C1-C10 alkyl group substituted with a rO—CO— group (wherein r is as defined above), a cyano group or an aminocarbonyl group, or a rO—CO—(rO—COCH₂)CH— group (wherein r is as defined above)}; an a₅-NHSO₂— group (wherein a₅ represents a C2-C10 alkyl group substituted with a C1-C10 alkoxy group); a r₀ON═CH— group (wherein r₀ is as defined above); a r₀NHCSNH— group (wherein r₀ is as defined above); a r₀NHC(—Sr₀′)═N— group (wherein r₀ is as defined above, r₀′ is the same as the different from r₀ and has the same meaning as r₀ has); or a (r₀O)₂P(═O)CH₂— group (wherein r₀ is as defined above);

p represents 1, 2 or 3, and when p is 2 or more, X_(a)S are the same or different;

Y_(a) represents a halogen atom, a nitro group, a r₀CO—NH— group (wherein r₀ is as defined above), a C1-C10 alkyl group or a C1-C10 alkoxy group;

q represents 0, 1 or 2, and when q is 2 or more, Y_(a)S are the same or different;

q_(a) represents a r_(a)-O— group {wherein r_(a) represents a hydrogen atom, a C1-C10 alkyl group, a C3-C10 alkenyl group, a C3-C10 alkynyl group, a C1-C10 alkyl group substituted with a r₀r₀′N—CH₂— group (wherein r₀ and r₀′ are as defined above), a rOCH₂— group (wherein r is as defined above), a r₀-CO— group (wherein r₀ is as defined above), a C1-C10 alkoxycarbonyl group, a carboxy group, an aminocarbonyl group or a cyano group, or a r₃-r₁- group (wherein r₃ represents a phenyl group or a pyridyl group, and r₁ is as defined above)}; a piperidino group; a morpholino group; or a r₄r₄′N— group (wherein r₄ and r₄′ are the same or different, and represent a hydrogen atom, a C1-C10 alkyl group, a C3-C10 alkenyl group, a C3-C10 alkynyl group, or a C2-C10 alkyl group substituted with a C1-C10 alkoxy group, provided that r₄ and r₄′ are not a hydrogen atom at the same time);

t_(a) represents a r_(b)- group (wherein r_(b) is the same as or different from r_(a), and has the same meaning as r_(a) has) or a r₃′-group (wherein r₃′ is the same as or different from r₃, and has the same meaning as r₃ has);

K_(a) represents a hydrogen atom, a halogen atom or a C1-C10 alkyl group, and L_(a) represents a hydrogen atom or a C1-C10 alkyl group; or

K_(a) and L_(a) together may form a C1-C10 alkylene group or a 1,3-butadienylene group;

the term “as defined above” used for the same symbols among plural substituents means that the plural substituents independently represent the same meaning as that described above and, among the plural substituents, although the selection range of substituents to be selected is the same, selected substituents may be the same or different as long as they are selected within the range;

7. A 2(1H)-pyridinone compound represented by the formula (VII):

wherein

x represents a methine group or a nitrogen group;

X_(a)′ is a substituent on a carbon atom, and represents a C1-C10 alkyl group substituted with a cyano group; a C1-C10 alkyl group substituted with a tetrahydropyran-4-ylidene group; a C2-C10 alkenyl group substituted with a cyano group; a C2-C10 alkenyl group substituted with a C1-C10 alkoxycarbonyl group; a C3-C10 alkynyl group substituted with a hydroxyl group; an a₀′-r₁-b-r₁′-group {wherein a₀′ represents a methyl group substituted with a C1-C10 alkylthio group, a C2-C10 alkenyl group, a r₂O—CO— group (wherein r₂ represents a C1-C10 alkyl group, or a C2-C10 alkyl group substituted with a hydroxyl group), a carboxyl group, a rr′N—CO— group (wherein r and r′ are the same or different, and represent a hydrogen atom or a C1-C10 alkyl group), an a₁-NH—CO— group (wherein a₁ represents a C2-C10 alkyl group substituted with a C1-C10 alkoxy group), a rr′N—CH₂— group (wherein r and r′ are as defined above), a r₀-O—CONH—CH₂— group (wherein r₀ represents a C1-C10 alkyl group), a r-OCH₂— group (wherein r is as defined above), a r₀-CO— group (wherein r₀ is as defined above), a cyano group, or a sulfomethyl group, r₁ represents a C1-C10 alkylene group, r₁′ represents a single bond or a C1-C10 alkylene group, and b represents an oxy group, a thio group, a sulfinyl group, a sulfonyl group or a imino group}; an a₂-y-CO—NH— group (wherein a₂ represents a C2-C10 alkyl group substituted with a C1-C10 alkoxy group, and y represents an oxy group or an imino group); a r₀O—COCO—NH— group (wherein r₀ is as defined above); an a′₃-CO—NH— group (wherein a′₃ represents a C1-C10 alkyl group substituted with a C1-10 alkoxy group); an a₄-NHCO— group {wherein a₄ represents a C1-C10 alkoxy group, or a C3-C10 alkenyloxy group, or a r₀-SO₂— group (wherein r₀ is as defined above), or a C2-C10 alkyl group substituted with a hydroxyl group or a C1-C10 alkoxy group, or a C1-C10 alkyl group substituted with a r₀-CO— group (wherein r is as defined above), a cyano group or an aminocarbonyl group, or a rO—CO-(rO—COCH₂)CH— group (wherein r is as defined above)}; an a₅-NHSO₂— group (wherein a₅ represents a C2-C10 alkyl group substituted with a C1-C10 alkoxy group); a r₀ON═CH— group (wherein r₀ is as defined above); a r₀NHCSNH— group (wherein r₀ is as defined above); a r₀NHC(—Sr₀′)═N— group (wherein r₀ is as defined above, r₀′ is the same as the different from r₀ and has the same meaning as r₀ has); or a (r₀O)₂P(═O)CH₂— group (wherein r₀ is as defined above);

Y_(a)′ represents a halogen atom, a C1-C10 alkyl group or a C1-C10 alkoxy group;

q′ represents 0 or 1;

q_(a)′ represents a r_(a)′-O— group {wherein r_(a)′ represents a hydrogen atom, or a C1-C10 alkyl group, or a C3-C10 alkenyl group, or a C3-C10 alkynyl group, or a C1-C10 alkyl group substituted with a hydroxymethyl group, a C1-C10 alkoxycarbonyl group, a carboxy group, an aminocarbonyl group or a cyano group, or a benzyl group}; or a r₅r₅′N— group (wherein r₅ and r₅′ represent a hydrogen atom, a C3-C10 alkynyl group, or a C2-C10 alkyl group substituted with a C1-C10 alkoxy group, provided that they are not a hydrogen atom at the same time);

t_(a)′ represents a r_(b)′-group {wherein r_(b)′ represents a hydrogen atom; a C1-C10 alkyl group; a C3-C10 alkenyl group; a C3-C10 alkynyl group; a C1-C10 alkyl group substituted with a methoxymethyl group, a C1-C10 alkoxycarbonyl group, a carboxy group, an aminocarbonyl group, a cyano group or a r₀-CO— group (wherein r₀ is as defined above); a benzyl group; a phenyl group; or a 2-pyridyl group};

the term “as defined above” used for the same symbols among plural substituents means that the plural substituents independently represent the same meaning as that described above and, among the plural substituents, although the selection range of substituents to be selected is the same, selected substituents may be the same or different as long as they are selected within the range;

8. A 2(1H)-quinolinone compound represented by the formula (VIII):

wherein

a represents a benzene ring or a pyridine ring;

x represents a methine group or a nitrogen atom;

X_(a) is a substituent on a carbon atom, and represents a C1-C10 alkyl group substituted with a cyano group; a C1-C10 alkyl group substituted with a tetrahydropyran-4-ylidene group; a C2-C10 alkenyl group substituted with a halogen atom or a cyano group; a C2-C10 alkenyl group substituted with a C1-C10 alkoxycarbonyl group; a C3-C10 alkynyl group substituted with a hydroxyl group; an a₀-r₁-b-r₁′-group {wherein a₀ represents a methyl group substituted with a C1-C10 alkylthio group, a methyl group substituted with a C1-C10 alkylsulfinyl group, a methyl group substituted with a C1-C10 alkylsulfonyl group, a C2-C10 alkenyl group, a C2-C10 alkynyl group, a r₂O—CO— group (wherein r₂ represents a C1-C10 alkyl group, or a C2-C10 alkyl group substituted with a hydroxyl group), a carboxyl group, a rr′N—CO— group (wherein r and r′ are the same or different, and represent a hydrogen atom or a C1-C10 alkyl group), an a₁-NH—CO— group (wherein a₁ represents a C2-C10 alkyl group substituted with a C1-C10 alkoxy group), an a₁′-CO— group (wherein a₁′ represents a morpholino group), a rr′N—CH₂— group (wherein r and r′ are as defined above), a r₀-(O)₁—CONH—CH₂— group (wherein r₀ represents a C1-C10 alkyl group, and 1 represents 0 or 1), a r-OCH₂— group (wherein r is as defined above), a r₀-CO— group (wherein r₀ is as defined above), a cyano group, or a sulfomethyl group, r₁ represents a C1-C10 alkylene group, r₁′ represents a single bond or a C1-C10 alkylene group, and b represents an oxy group, a thio group, a sulfinyl group, a sulfonyl group or a imino group}; an a₂-y-CO—NH— group (wherein a₂ represents a C2-C10 alkyl group substituted with a C1-C10 alkoxy group, and y represents an oxy group or an imino group); a r₀O—COCO—NH— group (wherein r₀ is as defined above); an a₃-z-NH— group (wherein a₃ represents a C2-C10 alkenyl group, or a C1-C10 alkyl group substituted with a C1-10 alkoxy group, a C1-C10 alkoxycarbonyl group, a carboxy group or a cyano group, and z represents a carbonyl group or a sulfonyl group); an a₄-NHCO— group {wherein a₄ represents a C1-C10 alkoxy group, or a C3-C10 alkenyloxy group, or a r₀-SO₂— group (wherein r₀ is as defined above), or a C2-C10 alkyl group substituted with a hydroxyl group or a C1-C10 alkoxy group, or a C1-C10 alkyl group substituted with a rO—CO— group (wherein r is as defined above), a cyano group or an aminocarbonyl group, or a rO—CO-(rO—COCH₂)CH— group (wherein r is as defined above)}; an a₅-NHSO₂— group (wherein a₅ represents a C2-C10 alkyl group substituted with a C1-C10 alkoxy group); a r₀ON═CH— group (wherein r₀ is as defined above); a r₀NHCSNH— group (wherein r₀ is as defined above); a r₀NHC(—Sr₀′)═N— group (wherein r₀ is as defined above, r₀′ is the same as the different from r₀ and has the same meaning as r₀ has); or a (r₀O)₂P(═O)CH₂— group (wherein r₀ is as defined above);

p represents 1, 2 or 3, and when p is 2 or more, X_(a)s are the same or different;

Y_(a) represents a halogen atom, a nitro group, a r₀CO—NH— group (wherein r₀ is as defined above), a C1-C10 alkyl group or a C1-C10 alkoxy group;

q represents 0, 1 or 2, and when q is 2 or more, Y_(a)s are the same or different;

q_(a) represents a r_(a)-O— group {wherein r_(a) represents a hydrogen atom, a C1-C10 alkyl group, a C3-C10 alkenyl group, a C3-C10 alkynyl group, a C1-C10 alkyl group substituted with a r₀r₀′N—CH₂— group (wherein r₀ and r₀′ are as defined above), a rOCH₂— group (wherein r is as defined above), a r₀-CO— group (wherein r₀ is as defined above), a C1-C10 alkoxycarbonyl group, a carboxy group, an aminocarbonyl group or a cyano group, or a r₃-r₁- group (wherein r₃ represents a phenyl group or a pyridyl group, and r₁ is as defined above)); a piperidino group; a morpholino group; or a r₄r₄′N— group (wherein r₄ and r₄′ are the same or different, and represent a hydrogen atom, a C1-C10 alkyl group, a C3-C10 alkenyl group, a C3-C10 alkynyl group, or a C2-C10 alkyl group substituted with a C1-C10 alkoxy group, provided that r₄ and r₄′ are not a hydrogen atom at the same time);

t_(a) represents a r_(b)- group (wherein r_(b) is the same as or different from r_(a), and has the same meaning as r_(a) has) or a r₃′-group (wherein r₃′ is the same as or different from r₃, and has the same meaning as r₃ has);

K_(a) represents a hydrogen atom, a halogen atom or a C1-C10 alkyl group, and L_(a) represents a hydrogen atom or a C1-C10 alkyl group; or

K_(a) and L_(a) together may form a C1-C10 alkylene group or a 1,3-butadienylene group;

the term “as defined above” used for the same symbols among plural substituents means that the plural substituents independently represent the same meaning as that described above and, among the plural substituents, although the selection range of substituents to be selected is the same, selected substituents may be the same or different as long as they are selected within the range;

9. A 2(1H)-quinolinone compound represented by the formula (IX):

wherein

X_(a)″ represents a C1-C10 alkoxy group substituted with a cyano group, a hydroxymethyl group, a carboxy group or a C1-C10 alkoxycarbonyl group; an a₆-CONH— group (wherein a₆ represents a C1-C10 alkyl group substituted with a C1-C10 alkoxy group, or a C2-C10 alkoxy group substituted with a C1-C10 alkoxy group); or an a₇-NHCO— group (wherein a₇ represents a methanesulfonyl group, or a C1-C10 alkyl group substituted with a cyano group, a C1-C10 alkoxy group or a C1-C10 alkoxycarbonyl group);

q_(a)″ represents a hydroxy group, a C1-C10 alkoxy group or a piperidino group; and

t_(a)″ represents a hydrogen atom or a C1-C10 alkyl group.

10. A 2(1H)-pyridinone compound represented by the formula (X):

wherein

X_(I) represents a C2-C4 alkenyl group substituted with a cyano group, an A_(I)-R_(I)—O— group [wherein A_(I) represents a C1-C4 alkylthio group, a C2-C4 alkenyl group, a C2-C4 alkynyl group, a C₁-C4 alkoxycarbonyl group, a carboxy group, a RR′N—CO— group (wherein R and R′ are the same or different, and represent a hydrogen atom or a C1-C4 alkyl group), a RR′N—CH₂— group (wherein R and R′ are as defined above), a R—OCH₂— group (wherein R is as defined above) or a cyano group, and R_(I) represents a C1-C4 alkylene group], an A_(II)-(y)_(m)-z-NH— group (wherein A_(II) represents a C2-C4 alkenyl group, or a C1-C4 alkyl group substituted with a C1-C4 alkoxy group, a C1-C4 alkoxycarbonyl group, a carboxy group or a cyano group, y represents an oxy group or an imino group, z represents a carbonyl group or a sulfonyl group, and m represents 0 or 1), or an A_(III)-NHCO— group (wherein A_(III) represents a methanesulfonyl group, or a C1-C4 alkyl group substituted with a hydroxy group, a C1-C4 alkoxy group, a C1-C4 alkoxycarbonyl group, a carboxy group or a cyano group);

a and b are the same or different, and represent a hydrogen atom or a C1-C4 alkyl group;

Y_(I) represents a halogen atom, a nitro group, a C1-C4 alkyl group or a C1-C4 alkoxy group;

n represents 0, 1 or 2, and when n is 2, Y_(I)s may be different;

11. A 2(1H)-pyridinone compound represented by the formula (XI):

wherein

X_(I)′ represents a C2-C4 alkenyl group substituted with a cyano group, an A_(I)′-R_(I)—O— group (wherein A_(I)′ represents a C1-C4 alkylthio group, a C2-C4 alkenyl group, a C2-C4 alkynyl group, a C1-C4 alkoxycarbonyl group, a carboxy group or a cyano group, and R_(I) represents a C1-C4 alkylene group), an A_(II)-(y)_(m)-z-NH— group (wherein A_(II) represents a C2-C4 alkenyl group, or a C1-C4 alkyl group substituted with a C₁-C4 alkoxy group, a C₁-C4 alkoxycarbonyl group, a carboxy group or a cyano group, y represents an oxy group or an imino group, z represents a carbonyl group or a sulfonyl group, and m represents 0 or 1), or an A_(III)-NHCO— group (wherein A_(III) represents a methanesulfonyl group, or a C1-C4 alkyl group substituted with a hydroxy group, a C1-C4 alkoxy group, a C1-C4 alkoxycarbonyl group, a carboxy group or a cyano group);

a and b are the same or different, and represent a hydrogen atom or a C1-C4 alkyl group;

Y_(I) represents a halogen atom, a nitro group, a C1-C4 alkyl group or a C1-C4 alkoxy group;

n represents 0, 1 or 2, and when n is 2, Y_(I)s may be different;

12. A 2(1H)-quinolinone compound represented by the formula (XII):

wherein

X_(I)′ represents a C2-C4 alkenyl group substituted with a cyano group, an A_(I)′-R_(I)—O— group (wherein A_(I)′ represents a C1-C4 alkylthio group, a C2-C4 alkenyl group, a C2-C4 alkynyl group, a C1-C4 alkoxycarbonyl group, a carboxy group or a cyano group, and R_(I) represents a C1-C4 alkylene group), an A_(II)-(y)_(m)-z-NH— group (wherein A_(II) represents a C2-C4 alkenyl group, or a C1-C4 alkyl group substituted with a C1-C4 alkoxy group, a C1-C4 alkoxycarbonyl group, a carboxy group or a cyano group, y represents an oxy group or an imino group, z represents a carbonyl group or a sulfonyl group, and m represents 0 or 1), or an A_(III)-NHCO— group (wherein A_(III) represents a methanesulfonyl group, or a C1-C4 alkyl group substituted with a hydroxy group, a C1-C4 alkoxy group, a C1-C4 alkoxycarbonyl group, a carboxy group or a cyano group);

a and b are the same or different, and represent a hydrogen atom, or a C1-C4 alkyl group;

Y_(I) represents a halogen atom, a nitro group, a C1-C4 alkyl group or a C1-C4 alkoxy group;

n represents 0, 1 or 2, and when n is 2, Y_(I)s may be different;

13. A 2(1H)-pyridinone compound represented by the formula (XIII):

wherein

X_(II) represents a carboxymethoxy group, a dimethylaminocarbonylmethoxy group, a 3-dimethylaminopropoxy group, a 2-hydroxyethoxy group, a cyanomethoxy group, a methoxyacetylamino group, a 2-methoxyethoxycarbonylamino group, a 2-methoxyethylaminocarbonyl group or a methoxycarbonylmethylaminocarbonyl group, and

a′ and b′ are the same or different, and represent a hydrogen atom or a methyl group;

14. A 2(1H)-quinolinone compound represented by the formula (XIV):

wherein

X_(II)′ represents a cyanomethoxy group, a methoxyacetylamino group, a 2-methoxyethylaminocarbonyl group or a methoxycarbonylmethylaminocarbonyl group, and

a′ and b′ are the same or different, and represent a hydrogen atom or a methyl group;

15. A 2(1H)-pyridinone compound represented by the formula (XV):

16. A 2(1H)-pyridinone compound represented by the formula (XVI):

17. A 2(1H)-pyridinone compound represented by the formula (XVII):

18. A 2(1H)-pyridinone compound represented by the formula (XVIII):

19. A 2(1H)-pyridinone compound represented by the formula (XIX):

20. A 2(1H)-pyridinone compound represented by the formula (XX):

21. A 2(1H)-pyridinone compound represented by the formula (XXI):

22. A 2(1H)-pyridinone compound represented by the formula (XXII):

23. A 2(1H)-pyridinone compound represented by the formula (XXIII):

24. A 2(1H)-pyridinone compound represented by the formula (XXIV):

25. A 2(1H)-pyridinone compound represented by the formula (XXV):

26. A 2(1H)-pyridinone compound represented by the formula (XXVI):

27. A 2(1H)-pyridinone compound represented by the formula (XXVII):

28. A 2(1H)-pyridinone compound represented by the formula (XXVIII):

29. A 2(1H)-pyridinone compound represented by the formula (XXIX)

30. A 2(1H)-pyridinone compound represented by the formula (XXX):

31. A 2(1H)-quinolinone compound represented by the formula (XXXI):

32. A 2(1H)-quinolinone compound represented by the formula (XXXII):

33. A 2(1H)-quinolinone compound represented by the formula (XXXIII):

34. A 2(1H)-quinolinone compound represented by the formula (XXXIV):

35. A 2(1H)-quinolinone compound represented by the formula (XXXV):

36. A 2(1H)-quinolinone compound represented by the formula (XXXVI):

37. A 2(1H)-quinolinone compound represented by the formula (XXXVII):

38. A 2(1H)-quinolinone compound represented by the formula (XXXVIII):

39. A benzaldehyde derivative represented by the formula (XXXIX-1):

wherein X_(b) represents a MeO—COCH₂NHCO— group, a MeOCH₂CH₂O—CO—NH— group, a MeOCH₂CH₂NH—CO—NH— group, a MeSO₂NH—CO— group, a NCCH₂NH—CO— group, a F₂C═CH— group, a MeO—CO-(MeO—COCH₂—)CH— group, a MeOCH₂CH₂NH—SO₂— group, a MeO—NHCO— group or a CH₂═CHCH₂O—NHCO— group,

the formula (XXXIX-2):

wherein X_(b)′ represents a MeOCH₂CO—NH— group or a MeOCH₂CH₂NH—CO— group,

the formula (XXXIX-3):

wherein X_(b)″ represents a MeSCH₂CH₂— group, a HOCH₂CH₂OCH₂— group or a NC—CH₂CH₂— group, or

the formula (XXXIX-4):

wherein X_(b)′″ represents a NCCH═CH— group, a H₂NCOCH₂O— group, a MeCOCH₂O— group, a CH₃O—COCH₂SCH₂— group, a tetrahydropyran-4-ylidenemethyl group, a CH₃O—COCO—NH— group or a (CH₃O)₂P(═O)CH₂— group; or a 6-formyl-2-[(2-methoxyethyl)aminocarbonyl]pyridine;

40. A benzaldehyde derivative represented by the formula (XL):

41. A benzaldehyde derivative represented by the formula (XLI):

42. A benzaldehyde derivative represented by the formula (XLII):

43. A benzaldehyde derivative represented by the formula (LXIII):

44. A benzaldehyde derivative represented by the formula (LXIV):

45. A benzaldehyde derivative represented by the formula (XLV):

46. A benzaldehyde derivative represented by the formula (XLVI):

47. A benzaldehyde derivative represented by the formula (XLVII):

48. A benzaldehyde derivative represented by the formula (XLVIII):

49. A benzaldehyde derivative represented by the formula (XLIX):

50. A benzaldehyde derivative represented by the formula (L):

51. A benzaldehyde derivative represented by the formula (LI):

52. A benzaldehyde derivative represented by the formula (LII):

53. A benzaldehyde derivative represented by the formula (LIII):

54. A benzaldehyde derivative represented by the formula (LIV):

55. A pyridinecarbaldehyde derivative represented by the formula (LV):

56. A benzaldehyde derivative represented by the formula (LVI):

57. A benzaldehyde derivative represented by the formula (LVII):

58. A benzaldehyde derivative represented by the formula (LVIII):

59. A process for producing a cinnamoyl compound represented by the formula (LIX-1):

wherein X_(b) represents a MeO—COCH₂NHCO— group, a MeOCH₂CH₂O—CO—NH— group, a MeOCH₂CH₂NH—CO—NH— group, a MeSO₂NH—CO—group, a NCCH₂NH—CO— group, a F₂C═CH— group, a MeO—CO-(MeO—COCH₂—)CH— group, a MeOCH₂CH₂NH—SO₂— group, a MeO—NHCO— group or a CH₂═CHCH₂O—NHCO— group, and q_(a), t_(a), K_(a) and L_(a) are as defined below, the formula (LIX-2):

wherein X_(b)′ represents a MeOCH₂CO—NH— group or a MeOCH₂CH₂NH—CO— group, and q_(a), t_(a), K_(a) and L_(a) are as defined below, the formula (LIX-3):

wherein X_(b)″ represents a MeSCH₂CH₂O— group, a HOCH₂CH₂OCH₂— group or a NC—CH₂CH₂— group, and q_(a), t_(a), K_(a) and L_(a) are as defined below, the formula (LIX-4):

wherein X_(b)′″ represents a NCCH═CH— group, a H₂NCOCH₂O— group, a MeCOCH₂O— group, a CH₃O—COCH₂SCH₂— group, a tetrahydropyran-4-ylidenemethyl group, a CH₃O—COCO—NH— group or a (CH₃O)₂P(═O)CH₂— group, and q_(a), t_(a), K_(a) and L_(a) are as defined below, or the formula (LIX-5):

wherein q_(a), t_(a), K_(a) and L_(a) are as defined below, which comprises reacting a benzaldehyde derivative represented by the formula (XXXIX-1), (XXXIX-2), (XXXIX-3) or (XXXIX-4) or 6-formyl-2-[(2-methoxyethyl)aminocarbonyl]pyridine according to the above item 39, with a compound represented by the formula (LIX):

wherein

q_(a) represents a r_(a)-O— group {wherein r_(a) represents a hydrogen atom; a C1-C10 alkyl group; a C3-C10 alkenyl group; a C3-C10 alkynyl group; a C1-C10 alkyl group substituted with a r₀r₀′N—CH₂— group (wherein r₀ and r₀′ are the same or different, and represent a C1-C10 alkyl group), a rOCH₂— group (wherein r represents a hydrogen atom or a C1-C10 alkyl group), a r₀-CO— group (wherein r₀ is as defined above), a C1-C10 alkoxycarbonyl group, a carboxy group, an aminocarbonyl group or a cyano group; or a r₃-r₁- group (wherein r₃ represents a phenyl group or a pyridyl group, and r₁ represents a C1-C10 alkylene group)}, a piperidino group, a morpholino group, or a r₄r₄′N— group (wherein r₄ and r₄′ are the same or different, and represent a hydrogen atom, a C1-C10 alkyl group, a C3-C10 alkenyl group, a C3-C10 alkynyl group, or a C2-C10 alkyl group substituted with a C1-C10 alkoxy group, provided that r₄ and r₄′ are not a hydrogen atom at the same time),

t_(a) represents a r_(b)- group (wherein r_(b) is the same as or different from r_(a), and has the same meaning as r_(a) has) or a r₃′-group (wherein r₃′ is the same as or different from r₃, and has the same meaning as r₃ has),

K_(a) represents a hydrogen atom, a halogen atom or a C1-C10 alkyl group, and L_(a) represents a hydrogen atom or a C1-C10 alkyl group, or

K_(a) and L_(a) together may form a C1-C10 alkylene group or a 1,3-butadienylene group, and

the term “as defined above (or below)” used for the same symbols among plural substituents means that the plural substituents independently represent the same meaning as that described above (or below) and, among the plural substituents, although the selection range of substituents to be selected is the same, selected substituents may be the same or different as long as they are selected within the range;

60. A process for producing a cinnamoyl compound represented by the formula (LX″):

wherein A, X_(c), Y_(a), p, q, r_(c), t_(c), K_(a) and L_(a) are as defined below, and the term “as defined above (or below)” used for the same symbols among plural substituents means that the plural substituents independently represent the same meaning as that described above (or below) and, among the plural substituents, although the selection range of substituents to be selected is the same, selected substituents may be the same or different as long as they are selected within the range; which comprises reacting a cinnamoyl compound represented by the formula (LX):

wherein

A represents a benzene ring or a pyridine ring,

X_(c) is a substituent on a carbon atom, and represents a C1-C10 alkyl group substituted with a cyano group; a C1-C10 alkyl group substituted with a tetrahydropyran-4-ylidene group; a C2-C10 alkenyl group substituted with a halogen atom or a cyano group; a C2-C10 alkenyl group substituted with a C1-C10 alkoxycarbonyl group; a C2-C10 alkynyl group substituted with a hydroxylmethyl group; an a_(0c)-r₁-b-r₁′-group (wherein a_(0c) represents a methyl group substituted with a C1-C10 alkylthio group, a methyl group substituted with a C1-C10 alkylsulfinyl group, a methyl group substituted with a C1-C10 alkylsulfonyl group, a C2-C1O alkenyl group, a C2-C10 alkynyl group, a r₂O—CO— group (wherein r₂ represents a C1-C10 alkyl group, or a C2-C10 alkyl group substituted with a hydroxyl group), a rr′N—CO— group (wherein r and r′ are the same or different, and represent a hydrogen atom or a C1-C10 alkyl group), an a₁-NH—CO— group (wherein a₁ represents a C2-C10 alkyl group substituted with a C1-C10 alkoxy group), an a₁′-CO— group (wherein a₁′ represents a morpholino group), a rr′N—CH₂— group (wherein r and r′ are as defined above), a r₀-(O)₁—CONH—CH₂— group (wherein r₀ represents a C1-C10 alkyl group, and 1 represents 0 or 1), a r-OCH₂— group (wherein r is as defined above), a r₀-CO— group (wherein r₀ is as defined above), or a cyano group, r₁ represents a C1-C10 alkylene group, r₁′ represents a single bond or a C1-C10 alkylene group, and b represents an oxy group, a thio group, a sulfinyl group, a sulfonyl group or a imino group}; an a₂-y-CO—NH— group (wherein a₂ represents a C2-C10 alkyl group substituted with a C1-C10 alkoxy group, and y represents an oxy group or an imino group); a r₀O—COCO—NH— group (wherein r₀ is as defined above); an a₃-z-NH— group (wherein a₃ represents a C2-C10 alkenyl group, or a C1-C10 alkyl group substituted with a C1-10 alkoxy group, a C1-C10 alkoxycarbonyl group or a cyano group, and z represents a carbonyl group or a sulfonyl group); an a₄-NHCO— group {wherein a₄ represents a C1-C10 alkoxy group, or a C3-C10 alkenyloxy group, or a r₀-SO₂— group (wherein r₀ is as defined above), or a C2-C10 alkyl group substituted with a hydroxyl group or a C1-C10 alkoxy group, or a C1-C10 alkyl group substituted with a r₀O—CO— group (wherein r₀ is as defined above), a cyano group or an aminocarbonyl group, or a r₀O—CO-(r₀O—COCH₂)CH— group (wherein r₀ is as defined above)}; an a₅-NHSO₂— group (wherein a₅ represents a C2-C10 alkyl group substituted with a C1-C10 alkoxy group); a r₀ON═CH— group (wherein r₀ is as defined above); a r₀NHCSNH— group (wherein r₀ is as defined above); a r₀NHC(—Sr₀′)═N— group (wherein r₀ is as defined above, r₀′ is the same as the different from r₀ and has the same meaning as r₀ has); or a (r₀O)₂P(═O)CH₂— group (wherein r₀ is as defined above);

p represents 1, 2 or 3, and when p is 2 or more, X_(c)s are the same or different;

Y_(a) represents a halogen atom, a nitro group, a r₀CO—NH— group (wherein r₀ is as defined above), a C1-C10 alkyl group or a C1-C10 alkoxy group;

q represents 0, 1 or 2, and when q is 2 or more, Y_(a)s are the same or different;

t_(c) represents a t_(c)′-group {wherein t_(c)′ represents a C1-C10 alkyl group; a C3-C10 alkenyl group; a C3-C10 alkynyl group; a C1-C10 alkyl group substituted with a r₀r₀′N—CH₂— group (wherein r₀ and r₀′ are as defined above), a rOCH₂— group (wherein r is as defined above), a r₀-CO— group (wherein r₀ is as defined above), a C1-C10 alkoxycarbonyl group, an aminocarbonyl group or a cyano group; or a r₃-r₁- group (wherein r₃ represents a phenyl group or a pyridyl group, and r₁ is as defined above)}, or a r₃- group (wherein r₃ is as defined above);

K_(a) represents a hydrogen atom, a halogen atom or a C1-C10 alkyl group, and L_(a) represents a hydrogen atom or a C1-C10 alkyl group, or

K_(a) and L_(a) together may form a C1-C10 alkylene group or a 1,3-butadienylene group, and

the term “as defined above” used for the same symbols among plural substituents means that the plural substituents independently represent the same meaning as that described above and, among the plural substituents, although the selection range of substituents to be selected is the same, selected substituents may be the same or different as long as they are selected within the range, with a compound represented by the formula (LX′) r_(c)-V  (LX′) wherein r_(c) is the same as or different from t_(c)′ and has the same meaning as t_(c)′ has, and V represents a leaving group, and the term “as defined above” used for the same symbols among plural substituents means that the plural substituents independently represent the same meaning as that described above and, among the plural substituents, although the selection range of substituents to be selected is the same, selected substituents may be the same or different as long as they are selected within the range;

61. A process for producing a cinnamoyl compound represented by the formula (LXI′):

wherein

A is as defined below,

X_(d)′ is a substituent on a carbon atom, and represents an a_(0d)′-r₁-b-r₁′-group (wherein a_(0d)′ represents a carboxy group, and r₁, r₁′ and b are as defined below), a HO—COCO—NH— group, an a_(3d)′-z-NH— group (wherein a_(3d)′ represents a C1-C10 alkyl group substituted with a carboxy group, and z is as defined below), or an a_(4d)′-NHCO— group (wherein a_(4d)′ represents a C1-C10 alkyl group substituted with a carboxy group, or a HO—CO—(HO—COCH₂)CH— group),

p is as defined below and, and when p is 2 or more, X_(d)′s are the same or different,

Y_(a) and q are as defined below,

q_(d)′ represents a r_(d)″-O— group {wherein r_(d)″ represents a hydrogen atom; a C1-C10 alkyl group; a C3-C10 alkenyl group; a C3-C10 alkynyl group; a C1-C10 alkyl group substituted with a r₀r₀′N—CH₂— group (wherein r₀ and r₀′ are as defined below), a rOCH₂— group (wherein r is as defined below), a r₀-CO— group (wherein r₀ is as defined below), a carboxy group, an aminocarbonyl group or a cyano group; or a r₃-r₁- group (wherein r₃ represents a phenyl group or a pyridyl group, and r₁ is as defined below)}, a piperidino group, a morpholino group, or a r₄r₄′N— group (wherein r₄ and r₄′ are as defined below, provided that they are not hydrogen atom at the same time),

t_(d)′ represents a r_(d)′″-group (wherein r_(d)′″ is the same as or different from r_(d)″, and has the same meaning as r_(d)″ has) or a r₃′-group (wherein r₃′ is as defined below),

K_(a) and L_(a) are as defined below, and

the term “as defined above (or below)” used for the same symbols among plural substituents means that the plural substituents independently represent the same meaning as that described above (or below) and, among the plural substituents, although the selection range of substituents to be selected is the same, selected substituents may be the same or different as long as they are selected within the range;

which comprises hydrolyzing a cinnamoyl compound represented by the formula (LXI):

wherein

A represents a benzene ring or a pyridine ring,

X_(d) is a substituent on a carbon atom, and represents an a_(0d)-r₁-b-r₁′-group {wherein a_(0d) represents a r₂O—CO— group (wherein r₂ represents a C1-C10 alkyl group, or a C2-C10 alkyl group substituted with a hydroxy group), r₁ represents a C1-C10 alkylene group, r₁′ represents a single bond or a C1-C10 alkylene group, and b represents an oxy group, a thio group, a sulfinyl group, a sulfonyl group or an imino group}, a r₀O—COCO—NH— group (wherein r₀ represents a C1-C10 alkyl group), an a_(3d)-z-NH— group (wherein a_(3d) represents a C1-C10 alkyl group substituted with a C1-C10 alkoxycarbonyl group, and z represents a carbonyl group or a sulfonyl group), or an a_(4d)-NHCO— group {wherein a_(4d) represents a C1-C10 alkyl group substituted with a r₀O—CO— group (wherein r₀ is as defined above), or a r₀O—CO-(r₀O—COCH₂)CH— group (wherein r₀ is as defined above)},

p represents 1, 2 or 3, and when p is 2 or more, X_(d)s are the same or different,

Y_(a) represents a halogen atom, a nitro group, a r₀CO—NH— group (wherein r₀ is as defined above), a C1-C10 alkyl group or a C1-C10 alkoxy group,

q represents 0, 1 or 2, and when q is 2 or more, Y_(a)s are the same or different;

q_(d) represents a r_(d)-O— group {wherein r_(d) represents a hydrogen atom, a C1-C10 alkyl group, a C3-C10 alkenyl group, a C3-C10 alkynyl group, a C1-C10 alkyl group substituted with a r₀r₀′N—CH₂— group (wherein r₀ is as defined above, and r₀′ is the same as or different from r₀ and has the same meaning as r₀ has), a rOCH₂— group (wherein r is as defined above), a r₀-CO— group (wherein r₀ is as defined above), a C1-C10 alkoxycarbonyl group, a carboxy group, an aminocarbonyl group or a cyano group, or a r₃-r₁- group (wherein r₃ represents a phenyl group or a pyridyl group, and r₁ is as defined above)}; a piperidino group; a morpholino group; or a r₄r₄′N— group (wherein r₄ and r₄′ represent a hydrogen atom, a C1-C10 alkyl group, a C3-C10 alkenyl group, a C3-C10 alkynyl group, or a C2-C10 alkyl group substituted with a C1-C10 alkoxy group, provided that they are not a hydrogen atom at the same time),

t_(d) represents a r_(d)′-group (wherein r_(d)′ is the same as or different from r_(d), and has the same meaning as r_(d) has) or a r₃′-group (wherein r₃′ is the same as or different from r₃, and has the same meaning as r₃ has),

K_(a) represents a hydrogen atom, a halogen atom or a C1-C10 alkyl group, and L_(a) represents a hydrogen atom or a C1-C10 alkyl group, or

K_(a) and L_(a) together may form a C1-C10 alkylene group or a 1,3-butadienylene group,

the term “as defined above” used for the same symbols among plural substituents means that the plural substituents independently represent the same meaning as that described above and, among the plural substituents, although the selection range of substituents to be selected is the same, selected substituents may be the same or different as long as they are selected within the range;

62. A process for producing a cinnamoyl compound represented by the formula (LXII″):

wherein A, X_(c), Y_(a), p, q, t_(c)′, K_(a) and L_(a) are as defined below, and the term “as defined above (or below)” used for the same symbols among plural substituents means that the plural substituents independently represent the same meaning as that described above (or below) and, among the plural substituents, although the selection range of substituents to be selected is the same, selected substituents may be the same or different as long as they are selected within the range; which comprises reacting a cinnamoyl compound represented by the formula (LXII):

wherein

A represents a benzene ring or a pyridine ring,

X_(c) is a substituent on a carbon atom, and represents a C1-C10 alkyl group substituted with a cyano group; a C1-C10 alkyl group substituted with a tetrahydropyran-4-ylidene group; a C2-C10 alkenyl group substituted with a halogen atom or a cyano group; a C2-C10 alkenyl group substituted with a C1-C10 alkoxycarbonyl group; a C2-C10 alkynyl group substituted with a hydroxylmethyl group; an a_(0c)-r₁-b-r₁′-group {wherein a_(0c) represents a methyl group substituted with a C1-C10 alkylthio group, a methyl group substituted with a C1-C10 alkylsulfinyl group, a methyl group substituted with a C1-C10 alkylsulfonyl group, a C2-C10 alkenyl group, a C2-C10 alkynyl group, a r₂O—CO— group (wherein r₂ represents a C1-C10 alkyl group, or a C2-C10 alkyl group substituted with a hydroxyl group), a rr′N—CO— group (wherein r and r′ are the same or different, and represent a hydrogen atom or a C1-C10 alkyl group), an a₁-NH—CO— group (wherein a₁ represents a C2-C10 alkyl group substituted with a C1-C10 alkoxy group), an a₁′-CO— group (wherein a₁′ represents a morpholino group), a rr′N—CH₂— group (wherein r and r′ are as defined above), a r₀-(O)₁—CONH—CH₂— group (wherein r₀ represents a C1-C10 alkyl group, and 1 represents 0 or 1), a r-OCH₂— group (wherein r is as defined above), a r₀-CO— group (wherein r₀ is as defined above), or a cyano group, r₁ represents a C1-C10 alkylene group, r₁′ represents a single bond or a C1-C10 alkylene group, and b represents an oxy group, a thio group, a sulfinyl group, a sulfonyl group or a imino group}; an a₂-y-CO—NH— group (wherein a₂ represents a C2-C10 alkyl group substituted with a C1-C10 alkoxy group, and y represents an oxy group or an imino group); a r₀O—COCO—NH— group (wherein r₀ is as defined above); an a₃-z-NH— group (wherein a₃ represents a C2-C10 alkenyl group, or a C1-C10 alkyl group substituted with a C1-10 alkoxy group, a C1-C10 alkoxycarbonyl group or a cyano group, and z represents a carbonyl group or a sulfonyl group); an a₄-NHCO— group (wherein a₄ represents a C1-C10 alkoxy group, a C3-C10 alkenyloxy group, a r₀-SO₂— group (wherein r₀ is as defined above), a C2-C10 alkyl group substituted with a hydroxyl group or a C1-C10 alkoxy group, a C1-C10 alkyl group substituted with a r₀O—CO— group (wherein r₀ is as defined above), a cyano group or an aminocarbonyl group, or a r₀O—CO-(r₀O—COCH₂)CH— group (wherein r₀ is as defined above)}; an a₅-NHSO₂— group (wherein a₅ represents a C2-C10 alkyl group substituted with a C1-C10 alkoxy group); a r₀ON═CH— group (wherein r₀ is as defined above); a r₀NHCSNH— group (wherein r₀ is as defined above); a r₀NHC(—Sr₀′)═N— group (wherein r₀ is as defined above, r₀′ is the same as the different from r₀ and has the same meaning as r₀ has); or a (r₀O)₂P(═O)CH₂— group (wherein r₀ is as defined above),

p represents 1, 2 or 3, and when p is 2 or more, X_(c)s are the same or different,

Y_(a) represents a halogen atom, a nitro group, a r₀CO—NH— group (wherein r₀ is as defined above), a C1-C10 alkyl group or a C1-C10 alkoxy group,

q represents 0, 1 or 2, and when q is 2 or more, Y_(a)s are the same or different;

K_(a) represents a hydrogen atom, a halogen atom or a C1-C10 alkyl group, and L_(a) represents a hydrogen atom or a C1-C10 alkyl group, or

K_(a) and L_(a) together may form a C1-C10 alkylene group or a 1,3-butadienylene group, and

the term “as defined above” used for the same symbols among plural substituents means that the plural substituents independently represent the same meaning as that described above and, among the plural substituents, although the selection range of substituents to be selected is the same, selected substituents may be the same or different as long as they are selected within the range, with a compound represented by the formula (LXII′): t_(c)′-V  (LXII′) wherein t_(c)′ represents a C1-C10 alkyl group, a C3-C10 alkenyl group, a C3-C10 alkynyl group, a C1-C10 alkyl group substituted with a r₀r₀′N—CH₂— group (wherein r₀ and r₀′ are as defined above), a rOCH₂— group (wherein r is as defined above), a r₀-CO— group (wherein r₀ is as defined above), a C1-C10 alkoxycarbonyl group, an aminocarbonyl group or a cyano group, or a r₃-r₁- group (wherein r₃ represents a phenyl group or a pyridyl group, and r₁ is as defined above), and V represents a leaving group, and

the term “as defined above” used for the same symbols among plural substituents means that the plural substituents independently represent the same meaning as that described above and, among the plural substituents, although the selection range of substituents to be selected is the same, selected substituents may be the same or different as long as they are selected within the range;

63. A process for producing a cinnamoyl compound represented by the formula (LXIII″):

wherein X_(e)′ represents an a_(0e)′-r₁″-b″-group {wherein a_(0e)′ represents an a_(0e)- group (wherein a_(0e) is as defined below), a 3-sulfopropyl group or a 4-sulfobutyl group, and r₁″ and b″ are as defined below}, and A, Y_(a), p, q, q_(e), t_(e), K_(a) and L_(a) are as defined below, and the term “as defined above (or below)” used for the same symbols among plural substituents means that the plural substituents independently represent the same meaning as that described above (or below) and, among the plural substituents, although the selection range of substituents to be selected is the same, selected substituents may be the same or different as long as they are selected within the range; which comprises reacting a cinnamoyl compound represented by the formula (LXIII):

wherein

A represents a benzene ring or a pyridine ring,

X_(e) is a substituent on a carbon atom, and represents a H-b″-group (wherein b″ represents an oxy group or a thio group),

p represents 1, 2 or 3 and, when p is 2 or more, X_(e)s are the same or different,

Y_(a) represents a halogen atom, a nitro group, a r₀CO—NH— group (wherein r₀ is a C1-C10 alkyl group), a C1-C10 alkyl group or a C1-C10 alkoxy group,

q represents 0, 1 or 2, and when q is 2 or more, Y_(a)s are the same or different;

q_(e) represents a r_(e)-O— group {wherein r_(e) represents a C1-C10 alkyl group, a C3-C10 alkenyl group, a C3-C10 alkynyl group, a C1-C10 alkyl group substituted with a r₀r₀′N—CH₂— group (wherein r₀ is as defined above, and r₀′ is the same as or different from r₀ and has the same meaning as r₀ has), a rOCH₂— group (wherein r represents a hydrogen atom or a C1-C10 alkyl group), a r₀-CO— group (wherein r₀ is as defined above), a C1-C10 alkoxycarbonyl group, an aminocarbonyl group or a cyano group, or a r₃-r₁- group (wherein r₃ represents a phenyl group or a pyridyl group, and r₁ represents a C1-C10 alkylene group)}; a piperidino group; a morpholino group; or a r₄r₄′N— group (wherein r₄ and r₄′ represent a hydrogen atom, a C1-C10 alkyl group, a C3-C10 alkenyl group, a C3-C10 alkynyl group, or a C2-C10 alkyl group substituted with a C1-C10 alkoxy group, provided that they are not a hydrogen atom at the same time),

t_(e) represents a r_(e)′-group (wherein r_(e)′ is the same as or different from r_(e), and has the same meaning as r_(e) has) or a r₃′-group (wherein r₃′ is the same as or different from r₃, and has the same meaning as r₃ has),

K_(a) represents a hydrogen atom, a halogen atom or a C1-C10 alkyl group, and L_(a) represents a hydrogen atom or a C1-C10 alkyl group, or

K_(a) and L_(a) together may form a C1-C10 alkylene group or a 1,3-butadienylene group, and

the term “as defined above” used for the same symbols among plural substituents means that the plural substituents independently represent the same meaning as that described above and, among the plural substituents, although the selection range of substituents to be selected is the same, selected substituents may be the same or different as long as they are selected within the range, with a compound represented by the formula (LXIII′) a_(0e)-r₁″-V″  (LXIII′) wherein

a_(0e) represents a methyl group substituted with a C2-C10 alkylthio group, a methyl group substituted with a C1-C10 alkylsulfinyl group, a methyl group substituted with a C1-C10 alkylsulfonyl group, a C2-C10 alkenyl group, a C2-C10 alkynyl group, a r₂O—CO— group (wherein r₂ represents a C1-C10 alkyl group, or a C2-C10 alkyl group substituted with a hydroxy group), a rr′N—CO— group (wherein r and r′ are the same or different, and represent a hydrogen atom or a C1-C10 alkyl group), an a₁-NH—CO— group (wherein a₁ represents a C2-C10 alkyl group substituted with a C1-C10 alkoxy group), an a₁′-CO— group (wherein a₁′ represents a morpholino group), a rr′N—CH₂— group (wherein r is as defined above, r′ is the same as or different from r and has the same meaning as r has), a r₀-(O)₁—CONH—CH₂— group (wherein r₀ is as defined above, and 1 represents 0 or 1), a r-OCH₂— group (wherein r is as defined above), a r₀-CO— group (wherein r₀ is as defined above) or a cyano group,

r₁″ is the same as or different from r₁ and has the same meaning as r₁ has, and V′ represents a leaving group or a hydroxy group, or 1,3-propanesultone or 1,4-butanesultone

the term “as defined above” used for the same symbols among plural substituents means that the plural substituents independently represent the same meaning as that described above and, among the plural substituents, although the selection range of substituents to be selected is the same, selected substituents may be the same or different as long as they are selected within the range;

64. Use of a compound according to any one of the above items 1 to 38 as an active ingredient for suppressing transcription of a Type I collagen gene;

65. A composition for suppressing transcription of a Type I collagen gene, which comprises a compound according to any one of the above items 1 to 38 and an inert carrier;

66. Use of a compound according to any one of the above items 1 to 38 as an active ingredient for decreasing expression of a Type I collagen gene to induce a reduction in accumulation of collagen and thereby improving tissue fibrosis;

67. A composition for improving tissue fibrosis, which comprises a compound according to any one of the above items 1 to 38 and an inert carrier;

68. A method for improving tissue fibrosis, which comprises administering an effective amount of a compound according to any one of the above items 1 to 38 to a mammal in need thereof;

69. Use of a compound according to any one of the above items 1 to 38 as an active ingredient for suppressing the activity of TGF-β;

70. A composition for suppressing the activity of TGF-β, which comprises a compound according to any one of the above items 1 to 38 and an inert carrier;

71. Use of a compound according to any one of the above items 1 to 38 as an active ingredient for inhibiting a promoting effect of TGF-β on transition to a hair regression phase to induce extension of a hair growth phase and thereby providing hair-growing effect;

72. A composition for hair growth which comprises a compound according to any one of the above items 1 to 38 and an inert carrier;

73. A method for growing hair, which comprises administering an effective amount of a compound according to any one of the above items 1 to 38 to a mammal in need thereof;

74. Use of a compound according to any one of the above items 1 to 38 as an active ingredient for treating chronic renal failure;

75. An agent for treating chronic renal failure, which comprises a compound according to any one of the above items 1 to 38 and an inert carrier;

76. Use of a compound according to the above item 2 as an active ingredient for suppressing transcription of a Type I collagen gene;

77. A composition for suppressing transcription of a Type I collagen gene, which comprises a compound according to the above item 2 and an inert carrier;

78. A composition for suppressing transcription of a Type I collagen gene, which comprises a compound according to the above item 3 and an inner carrier;

79. Use of a compound according to the above item 3 as an active ingredient for decreasing expression of a Type I collagen gene to induce a reduction in accumulation of collagen and thereby improving tissue fibrosis;

80. A composition for suppressing transcription of a Type I collagen gene, which comprises a compound according to the above item 4 and an inert carrier;

81. Use of a compound according to the above item 4 as an active ingredient for decreasing expression of a Type I collagen gene to induce a reduction in accumulation of collagen and thereby improving tissue fibrosis;

82. Use of a compound according to the above item 10 as an active ingredient for suppressing transcription of a Type I collagen gene;

83. A composition for suppressing transcription of a Type I collagen gene, which comprises a compound according to the above item 10 and an inert carrier;

84. Use of a compound according to the above item 11 as an active ingredient for suppressing transcription of a Type I collagen gene;

85. A composition for suppressing transcription of a Type I collagen gene, which comprises a compound according to the above item 11 and an inert carrier;

86. Use of a compound according to the above item 12 as an active ingredient for suppressing transcription of a Type I collagen gene;

87. A composition for suppressing transcription of a Type I collagen gene, which comprises a compound according to the above item 12 and an inert carrier;

88. Use of a compound according to any one of the above items 5 to 9 as an active ingredient for suppressing transcription of a Type I collagen gene;

89. A composition for suppressing transcription of a Type I collagen gene, which comprises a compound according to any one of the above items 5 to 9 and an inert carrier;

90. Use of a compound according to the above item 13 or 14 as an active ingredient for suppressing transcription of a Type I collagen gene;

91. A composition for suppressing transcription of a Type I collagen gene, which comprises a compound according to the above item 13 or 14 and an inert carrier;

92. Use of a compound according to any one of the above items 15 to 38 as an active ingredient for suppressing transcription of a Type I collagen gene;

93. A composition for suppressing transcription of a Type I collagen gene, which comprises a compound according to any one of the above items 15 to 38 and an inert carrier; and the like.

BEST MODE FOR CARRYING OUT THE INVENTION

The present invention will be explained in detail below.

In the present invention, a saturated hydrocarbon group in an alkyl group, a haloalkyl group, an alkoxy group, an alkoxycarbonyl group, an alkylthio group, an alkylsulfinyl group, an alkylsulfonyl group and an alkylene group may be branched, and a part or all of carbon atoms thereof may form a ring. An unsaturated hydrocarbon group in an alkenyl group, an alkenyloxy group, an alkynyl group, an alkynyloxy group, an alkenylene group and an alkynylene group may be branched, a part or all of carbon atoms thereof may form a ring, and the number of unsaturated bonds thereof may be singular or plural.

In the present invention, examples of an alkyl group include a methyl group, an ethyl group, an isopropyl group, a cyclohexyl group, a cyclopropylmethyl group and the like. Examples of a haloalkyl group include a 2,2,2-trifluoroethyl group and the like. Examples of an alkoxy group include a methoxy group, an ethoxy group, a cyclopentyloxy group, a 2-cyclohexylethoxy group and the like. Examples of an alkylthio group include a methylthio group and the like. Examples of an alkylsulfinyl group include a methylsulfinyl group and the like. Examples of an alkylsulfonyl group include a methylsulfonyl group and the like. Examples of an alkylene group include a methylene group, an ethylethylene group, a 1,4-cyclohexylene group and the like. Examples of an alkenyl group include a vinyl group, a 2-propenyl group, a 3-methyl-2-butenyl group, a 1,3-butadienyl group, a 3-cyclohexenyl group and the like. Examples of an alkynyl group include an ethynyl group, a 2-propynyl group, a 2-penten-4-ynyl group and the like. Examples of an alkenylene group include a vinylene group, a propenylene group, a 1,3-butadienylene group and the like. Examples of an alkynylene group include an ethynylene group, a propynylene group and the like.

In the present invention, examples of a halogen atom include a fluorine atom, a chlorine atom, a bromine atom and an iodine atom.

In the present invention, a pyridyl group includes a 2-pyridyl group, a 3-pyridyl group and a 4-pyridyl group. A furyl group includes a 2-furyl group and a 3-furyl group. A thienyl group includes a 2-thienyl group and a 3-thienyl group. A naphthyl group includes a 1-naphthyl group and a 2-naphthyl group.

In the present invention, examples of a leaving group include an alkylsulfonyloxy group such as a mesyloxy group and the like, an arylsulfonyloxy group such as a tosyloxy group and the like, an alkoxysulfonyloxy group such as a methoxysulfonyloxy group and the like, and a halogen atom such as a bromine atom and the like.

When the A ring is a pyridine ring in cinnamoyl compounds represented by the formulas (I), (II), (III) and (IV) (hereinafter, referred to as present compound (I), (II), (III) and (IV), respectively, in some cases), when the a ring is a pyridine ring in a cinnamoyl compound represented by the formula (V), a 2(1H)-pyridinone compound represented by the formula (VI) and a 2(1H)-quinolinone compound represented by the formula (VIII) (hereinafter, referred to as the present compound (V), (VI) and (VIII), respectively, in some cases), and when x is a nitrogen atom in a 2(1H)-pyridinone compound represented by the formula (VII) (hereinafter, referred to as the present compound (VII) in some cases), N-oxide thereof is included.

In the present compounds (V), (VI), (VII) and (VIII), when x is a methine group, the methine group has no substituent.

The present compounds (I) to (VIII), a 2(1H)-quinolinone compound represented by the formula (IX), a 2(1H)-pyridinone compound represented by the formula (X), a 2(1H)-pyridinone compound represented by the formula (XI), a 2(1H)-quinolinone compound represented by the formula (XII), a 2(1H)-pyridinone compound represented by the formula (XIII) and a 2(1H)-quinolinone compound represented by the formula (XIV) (hereinafter, referred to as the present compound (IX), (X), (XI), (XII), (XIII) and (XIV), respectively, in some cases), 2(1H)-pyridinone compounds represented by the formulas (XV) to (XXX) (hereinafter, referred to as the present compound (XV) to (XXX), respectively, in some cases) and 2(1H)-quinolinone compounds represented by formulas (XXXI) to (XXXVIII) (hereinafter, referred to as the present compound (XXXI) to (XXXVIII), respectively, in some cases) include their pharmacologically acceptable salts. Pharmacologically acceptable salts include salts with an inorganic acid, salts with an organic acid, salts with an inorganic base or salts with an organic base, of the present compounds (I) to (XXXVIII) (hereinafter, referred to as the present compound in some cases). Examples of a salt with an inorganic acid include hydrochloride, hydrobromide and the like Examples of a salt with an organic acid include acetate, benzoate and the like Examples of a salt with an inorganic base include a potassium salt, a sodium salt and the like. Examples of a salt with an organic base include a pyridine salt, a morpholine salt and the like.

In the present compound (II), X_(A0), Y_(A0), Q_(A0), K_(A0), L_(A0) and T_(A0) are independently represented by groups represented by D₁, D₂, D₃, D₄, D₅, R₀, R₀′, R₀″, R₁, R₁′, R₁″, R₂, R₂′, R₃, R₄, R₄′, R₅, R₆, A₁, A₂, A₃, A₄, A₅, A₆, A₇, A₇′, A₇″, A₈, A₈′, A₉, A₉′, A₉″, A₁₀, A₁₀′, A₁₁, B, B′, B₀, B₁, B₁′, B₂, B₂′, B₃, B₃′, B₄, B₄′, B₅, B₆, (a₀), (b₀), (c₀), (d₀), (e₀), M_(a), M_(a)′, M_(a)″, M_(a)′″, M_(a)″″, M_(b0), M_(c0), M_(d0), R_(a0), R_(b), R_(c), R_(d), R_(d)′, R_(e), R_(e)′, R_(e)″, R_(e)′″, B_(a), B_(b), B_(c), Y_(a), Y_(a)′, Y_(b), Y_(b)″, Y_(b)″, Y_(c) and Y_(c)′, and integers represented by k, k′, l, m, m′, n and n′.

In the present compound (III), X_(A), Y_(A), Q_(A), K_(A), L_(A) and T_(A) are independently represented by groups represented by D₁, D₂, D₃, D₄, D₅, R₀, R₀′, R₀″, R₁, R₁′, R₁″, R₂, R₂′, R₃, R₄, R₄′, R₅, R₆, A₁, A₂, A₃, A₄, A₅, A₆, A₇, A₇′, A₇″, A₈, A₈′, A₉, A₉′, A₉″, A₁₀, A₁₀′, A₁₁, B, B′, B₀, B₁, B₁′, B₂, B₂′, B₃, B₃′, B₄, B₄′, B₅, B₆, (a), (b), (c), (d), (e), M_(a), M_(a)′, M_(a)″, M_(a)′″, M_(a)″″, M_(b), M_(c), M_(d), R_(a), R_(b), R_(c), R_(d), R_(d)′, R_(e), R_(e)′, R_(e)″, R_(e)′″, B_(a), B_(b), B_(c), Y_(a), Y_(a)′, Y_(b), Y_(b)′, Y_(b)″, Y_(c) and Y_(c)′, and integers represented by k, k′, l, m, m′, n and n′.

In the present compounds (IV), (V), (VI) and (VIII), X_(a), Y_(a), q_(a) and t_(a) are independently represented by groups represented by a₀, a₁, a₁′, a₂, a₃, a₄, a₅, b, r, r′, r₀, r₀′, r₁, r₁′, r₂, r₃, r₃′, r₄, r₄′, r₁, r_(b), y and z, and an integer represented by 1.

In the present compound (VII), X_(a)′, Y_(a)′, q_(a)′ and t_(a)′ are independently represented by groups represented by a₀′, a₁, a₂, a₃′, a₄, a₅, b′, r, r′, r₀, r₁, r₁′, r₂, r₅, r₅′, r_(a)′, r_(b)′ and y.

In the present invention, X_(c), Y_(a), r_(c) and t_(c) in (LX), (LX′) and (LX″) are independently represented by groups represented by a_(0c), a₁, a₁′, a₂, a₃, a₄, a₅, b, r, r′, r₀, r₀′, r₁, r₁′, r₂, r₃, y and z, and an integer represented by 1.

In the present invention, X_(d), X_(d)′, Y_(a), q_(d), t_(d), q_(d)′ and t_(d)′ in (LXI) and (LXI′) are independently represented by groups represented by a_(0d), a_(0d)′, a_(3d), a_(3d)′, a_(4d), a_(4d)′, b, r₀, r_(o)′, r₁, r₁′, r₂, r₃, r₃′, r₄, r₄′, r_(d), r_(d)′, r_(d)″, r_(d)′″, y and z.

In the present invention, X_(c), Y_(a) and t_(c)′ in (LXII) and (LXII″) are independently represented by groups represented by a_(0c), a₁, a₁′, a₂, a₃, a₄, a₅, b, r, r′, r₀, r₀′, r₁, r₁′, r₂, r₃, y and z, and a integer represented by 1.

In the present invention, X_(e), X_(e)′, Y_(a), q_(e) and t_(e) in (LXIII) and (LXIII″) are independently represented by groups represented by a_(0e), a₁, a₁′, b″, r, r′, r₀, r₀′, r₁, r₂, r₃, r₃′, r₄, r₄′, r_(e) and r_(e)′.

In the Y₀ group of substituents which may be selected as Y_(α) of the present compound (I), the “6 to 10-membered aryl group” represents a monocyclic or fused aromatic hydrocarbon group, and includes a phenyl group, a 1-naphthyl group, a 2-naphthyl group, a 6-indanyl group and the like. The “5 to 10-membered heteroaryl group” represents a monocyclic or fused aromatic heterocyclic group, and includes a 2-furyl group, a 3-furyl group, a 2-thienyl group, a 3-thienyl group, a 2-pyridyl group, a 3-pyridyl group, a 4-pyridyl group, a 2-quinolyl group and the like. The “3 to 10-membered cyclic hydrocarbon or heterocyclic group optionally containing an unsaturated bond” includes a monocyclic ring or a fused ring, for example, includes a 2-cyclohexenyl group, a 2-morpholinyl group, a 4-piperidyl group and the like, and may be substituted with a single or same or different plural aforementioned M_(a)- groups.

In the Z₀ group of substituents which may be selected as Y_(α) of the present compound (I), the “group which is fused to the A ring” may have single or same or different plural atoms or groups selected from a halogen atom, a C1-C10 alkoxy group, a C3-C10 alkenyloxy group, a C3-C10 alkynyloxy group, a carbonyl group, a thiocarbonyl group, an oxy group, a thio group, a sulfinyl group and a sulfonyl group.

In R_(a0) of the E₀ group of substituents which may be selected as X_(A0) of the present compound (II), the “optionally substituted 5 to 7-membered aryl group or heteroaryl group” represents a monocyclic or fused aromatic hydrocarbon group or a monocyclic or fused aromatic heterocyclic group, and includes a phenyl group, a 1-naphthyl group, a 2-naphthyl group, a 6-indanyl group, a 2-furyl group, a 3-furyl group, a 2-thienyl group, a 3-thienyl group, a 2-pyridyl group, a 3-pyridyl group, a 4-pyridyl group, a 2-quinolyl group and the like. Said group may be substituted with single or same or different plural aforementioned M_(a)- groups.

In (d₀) of the Y₀ group of substituents which may be selected as Y_(α) and Y_(A0) of the present compounds (I) and (II), the “5 to 12-membered hydrocarbon ring which is substituted with a carbonyl group or a thiocarbonyl group and further which may be optionally substituted with an oxy group, a thio group, a —NR₁— group (wherein R₁ is as defined above), a sulfinyl group or a sulfonyl group” represents a 5 to 12-membered hydrocarbon ring in which one or more carbon atoms are substituted with a carbonyl group or a thiocarbonyl group and further one or more carbon atoms may be substituted with a group or groups, which may be the same or different, selected from an oxy group, a thio group, a —NR₁— group (wherein R₁ is as defined above), a sulfinyl group and a sulfonyl group.

In (e₀) of the Y₀ group of substituents which may be selected as Y_(α) and Y_(A0) of the present compounds (I) and (II), the “5 to 12-membered hydrocarbon ring optionally substituted with a carbonyl group, a thiocarbonyl group, an oxy group, a thio group, a —NR₁— group (wherein R₁ is as defined above), a sulfinyl group or a sulfonyl group” represents a 5 to 12-membered hydrocarbon ring in which one or more carbon atoms may be substituted with a group or groups, which may be the same or different, selected from a carbonyl group, a thiocarbonyl group, an oxy group, a thio group, a —NR₁— group (wherein R₁ is as defined above), a sulfinyl group and a sulfonyl group.

In (a) of the B group of substituents which may be selected as X_(A) of the present compound (III), the “C2-C10 alkylene group optionally substituted with an oxy group, a thio group, a sulfinyl group, a sulfonyl group or a —NR₁′— group (wherein R₁′ is as defined above)” represents a C2-C10 alkylene group in which one or more carbon atoms may be substituted with a group or groups, which may be the same or different, selected from an oxy group, a thio group, a sulfinyl group, a sulfonyl group and a —NR₁′— group (wherein R₁′ is as defined above). The “C3-C10 alkenylene group optionally substituted with an oxy group, a thio group, a sulfinyl group, a sulfonyl group or a —NR₁′— group (wherein R₁′ is as defined above)” represents a C3-C10 alkenylene group in which one or more carbon atoms may be substituted with a group or groups, which may be the same or different, selected from an oxy group, a thio group, a sulfinyl group, a sulfonyl group and a —NR₁′— group (wherein R₁′ is as defined above).

In (b) of the D group of substituents which may be selected as X_(A), of the present compound (III), the “C1-C10 alkylene group optionally substituted with a methyl group, an oxy group, a thio group, a sulfinyl group, a sulfonyl group or a —NR₁— group (wherein R₁ is as defined above)” represents a C2-C10 alkylene group in which one or more carbon atoms may be substituted with a methyl group or in which one or more carbon atoms may be substituted with a group or groups, which may be the same or different, selected from an oxy group, a thio group, a sulfinyl group, a sulfonyl group and a —NR₁— group (wherein R₁ is as defined above). The “C2-C10 alkenylene group optionally substituted with a methyl group, an oxy group, a thio group, a sulfinyl group, a sulfonyl group or a —NR₁— group (wherein R₁ is as defined above)” represents a C2-C10 alkenylene group in which one or more carbon atoms may be substituted with a methyl group or in which one or more carbon atoms may be substituted with a group or groups, which may be the same or different, selected from an oxy group, a thio group, a sulfinyl group, a sulfonyl group and a —NR₁— group (wherein R₁ is as defined above).

Groups belonging to the X₀ group, the Y₀ group and the Z₀ group which may be selected as Y_(α) of the present compound (I) will be exemplified in the following Table X, Table Y and Table Z, respectively.

Groups belonging to the A₀ group, the B₀ group, the C₀ group, the D₀ group, the E₀ group, the F₀ group, the G₀ group, the H₀ group, the I₀ group, the J₀ group, the K₀ group, the L₀ group, the M₀ group and the N₀ group which may be selected as X_(A0) of the present compound (II) will be exemplified in the following Table A, Table B, Table C, Table D, Table E, Table F, Table G, Table H, Table I, Table J, Table K, Table L, Table M and Table N, respectively. Groups belonging to the X₀ group, the Y₀ group and the Z₀ group which may be selected as Y_(A0) will be exemplified in the following Table X, Table Y and Table Z, respectively. Q₀ and T₀ will be exemplified in the following Table Q and Table T, respectively.

Groups belonging to the A group, the B group, the C group, the D group, the E group, the F group, the G group, the H group, the I group, the J group, the K group, the L group, the M group and the N group which may be selected as X_(A) of the present compound (III) will be exemplified in the following Table A, Table B, Table C, Table D, Table E, Table F, Table G, Table H, Table I, Table J, Table K, Table L, Table M and Table N, respectively. Groups belonging to the X group, the Y group and the Z group which may be selected as Y_(A) will be exemplified in the following Table X, Table Y and Table Z, respectively. Q and T will be exemplified in the following Table Q and Table T.

Groups belonging to the aforementioned A₀ to N₀ groups and A to N groups will be exemplified in the following Table A to Table N. When said groups have geometrical isomers, all of the geometrical isomers are included, and when said groups have tautomers, all of the tautomers are included.

Groups belonging to the A₀ group and the A group will be exemplified in Table A.

TABLE A No. Group A-1 —CH₂ONH₂ A-2 —CH₂ON(CH₃)₂ A-3 —CH₂ONHCOCH₃ A-4 —CH₂NHOCH₂CH═CH₂ A-5 —CH₂CN A-6 —CH₂CH₂CN A-7 —CH₂CH₂C(═NH)NH₂ A-8 —CH₂CH₂C(═NCH₂C≡CH)N(CH₃)₂ A-9 —CH₂C(═NH)NHCOCH₃ A-10 —CH₂C(═NOCOCH₃)—NH₂ A-11 —CH₂C(═NCOCH₃)—OCH₃ A-12 —CH₂CSNH₂ A-13 —CH₂NO₂ A-14 —CH₂SO₃H A-15 —SO₃H

Groups belonging to the B₀ group and the B group will be exemplified in Table B.

TABLE B No. Group B-1

B-2

B-3

B-4

B-5

B-6

Groups belonging to the C₀ group and the C group will be exemplified in Table C.

TABLE C No. Group C-1 —CH═CF₂ C-2 —CH═CHOCH₃ C-3 —CH═CHSCH₃ C-4 —CH═CHSOCH₃ C-5 —CH═CHSO₂CH₃ C-6 —CH═CHCH₂OH C-7 —CH═CHCH₂OCOCH₃ C-8 —CH═CHCHO C-9 —CH═CHCH═NCH₂CH═CH₂ C-10 —CH═CHCH═NOH C-11 —CH═CHCH═NOCH₂COOCH₃ C-12 —CH═CHCH═NOCH₂CN C-13 —CH═CHCH═NN(CH₃)₂ C-14 —CH═CHCH═NNHCOCH₃ C-15 —CH═CHCOCH₃ C-16 —CH═C(CH₃)COCH₃ C-17 —CH═CHCOCF₃ C-18 —CH═CHCH₂ON(CH₃)₂ C-19 —CH═CHCH₂ON(SO₂CH₃)CH₃ C-20 —CH═CHCH₂N(CH₂CH═CH₂)₂ C-21 —CH═CHCH₂N(OH)CH₃ C-22 —CH═CHNHCOCH₃ C-23 —CH═CHCN C-24 —CH═CHC(═NH)N(CH₃)₂ C-25 —CH═CHC(═NH)NHOCH₃ C-26 —CH═CHCSNH₂ C-27 —CH═CHNO₂ C-28 —CH═CHSO₃H

Groups belonging to the D₀ group and the D group will be exemplified in Table D.

TABLE D No. Group D-1

D-2

D-3 —C≡CI D-4 —C≡CCH₂SCH₃ D-5 —C≡CC(CH₃)₂OH D-6 —C≡CCH₂OCOOCH₃ D-7 —C≡CCH═NCH₃ D-8 —C≡CCH═NOCH₃ D-9 —C≡CCH═NN(CH₃)₂ D-10 —C≡CCH₂ON(CH₃)₂ D-11 —C≡CCH₂N(CH₃)₂ D-12 —C≡CCH₂CH₂NO₂

Groups belonging to the E₀ group and the E group will be exemplified in Table E.

TABLE E No. Group E-1 —CH═CHCOOCH₃ E-2 —CH═CHCOOC₂H₅ E-3 —CH═CHCOOCH₂CH₂Cl E-4 —CH═CHCOOCH₂CF₃ E-5 —CH═CHCOOCH₂CH═CH₂ E-6 —CH═CHCOOCH₂C≡CH E-7

E-8

E-9 —CH═CHCOOCH₂CH₂OCH₃ E-10 —CH═CHCOOCH₂CH₂SCH₃ E-11 —CH═CHCOOCH₂CH₂SOCH₃ E-12 —CH═CHCOOCH₂CH₂SO₂CH₃ E-13 —CH═CHCOOCH₂CH₂OH E-14 —CH═CHCOOCH₂CH₂OSO₂N(CH₃)₂ E-15 —CH═CHCOOCH₂CH₂COCH₃ E-17 —CH═CHCOOCH₂CH₂ON(CH₃)₂ E-18 —CH═CHCOOCH₂CH₂N(CH₃)₂ E-19 —CH═CHCOOCH₂CH₂N(OC₂H₅)C₂H₅ E-20 —CH═CHCOOCH₂CH₂NHCOCH₃ E-21 —CH═CHCOOCH₂CH₂N(CH₃)COCH₃ E-22 —CH═CHCOOCH₂CH₂NHCOOCH₂CH₂OCH₃ E-23 —CH═CHCOOCH₂CH₂NHCOSCH₂CH═CH₂ E-24 —CH═CHCOOCH₂CH₂NHCONHC₂H₅ E-25 —CH═CHCOOCH₂CH₂NHCON(CH₃)₂ E-26 —CH═CHCOOCH₂CH₂NHCON(OCH₃)CH₃ E-27 —CH═CHCOOCH₂CH₂NHCSNHCH₂CH₂Cl E-28 —CH═CHCOOCH₂CH₂NHSO₂N(CH₃)₂ E-29 —CH═CHCOOCH₂CH₂CN E-30 —CH═CHCOOCH₂CH₂NO₂ E-31 —CH═CHCOOCH₂CH₂SO₃H E-32

E-33 —CH═CHCONHCH₂CH₂SO₂N(CH₃)₂ E-34 —CH═CHCOSCH₃ E-35 —CH═CHCON(CH₃)CH₂C≡CH E-36 —CH═CHCON(OCH₃)CH₃ E-37 —CH═CHCONHOCH₃ E-38 —CH═CHCONHOCH₂CH═CH₂ E-39 —CH═CHCOOCH₂COOCH₃ E-40 —CH═CHCOSCH₂COOCH₃ E-41 —CH═CHCONHCH₂COOCH₃ E-42 —CH═CHCONHCH₂CON(CH₃)₂ E-43 —CH═CHCONHCH₂CN E-44 —CH═CHCONHCH₂C(═NH)N(CH₃)CH₂CH═CH₂ E-45 —CH═CHCONHCH₂C(═NH)NHOH E-46 —CH═CHCONHSO₂CH₃ E-47

E-48

E-49

E-50

E-51

E-52

E-53

E-54

E-55

E-56

E-57

E-58

E-59 —CH═CHCONHN(CH₃)₂ E-60 —CH═CHCONHNHCOOC₂H₅ E-61 —CH═CHCONHNHCSNH(c)C₆H₁₁ E-62 —CH═CFCOOCH₃

Groups belonging to the F₀ group and the F group will be exemplified in Table F.

TABLE F No. Group F-1 —OCH₂CH₂OH F-2 —OCH₂CH₂CH₂OH F-3 —CH₂OCH₂CH₂OH F-4 —OCH₂CH₂OCON(CH₃)₂ F-5 —OCH₂CH₂ONH₂ F-6 —OCH₂CH₂N(CH₃)₂ F-7 —OCH₂CH₂CH₂N(CH₃)₂ F-8 —OCH₂CH₂N(OCH₃)CH₃ F-9 —OCH₂CH₂NH₂ F-10 —OCH₂CH₂NHCOCH₃ F-11 —OCH₂CH₂N(CH₃)COCH₃ F-12 —OCH₂CH₂NHCOO(t)C₄H₉ F-13 —OCH₂CH₂NHCOSCH₂CH═CH₂ F-14 —OCH₂CH₂NHCONHC₂H₅ F-15 —OCH₂CH₂NHCON(CH₃)₂ F-16 —OCH₂CH₂NHCON(OCH₃)CH₃ F-17 —OCH₂CH₂NHCSNHCH₂CH₂Cl F-18 —OCH₂CH₂NO₂ F-19 —OCH₂CH₂SO₃H F-20 —OCH₂CH₂CH₂SO₃H F-21 —OCH₂CH₂CH₂CH₂SO₃H F-22 —OCH₂CH₂NHSO₂N(CH₃)₂ F-23

F-24 —OCH₂CH₂OCH₃ F-25 —OCH₂CH₂SCH₃ F-26 —OCH₂CH₂SOCH₃ F-27 —OCH₂CH₂SO₂CH₃ F-28 —OCH₂CN F-29 —OCH₂C(═NH)NH₂ F-30 —OCH₂CSNH₂ F-31 —OCH₂COCH₃ F-32 —OCH₂COCF₃ F-33 —OCH₂CHO F-34 —OCH₂CH═NOCH₂C≡CH F-35 —OCH₂CH═NN(CH₃)₂ F-36 —OCH₂COOH F-37 —OCH₂COOCH₃ F-38 —OCH₂COOCH₂CH₂Cl F-39 —OCH₂COOCH₂CH═CH₂ F-40 —OCH₂COOCH₂C≡CH F-41

F-42

F-43 —OCH₂COOCH₂CH₂OCH₃ F-44 —OCH₂COOCH₂CH₂SCH₃ F-45 —OCH₂COOCH₂CH₂SOCH₃ F-46 —OCH₂COOCH₂CH₂SO₂CH₃ F-47 —OCH₂COOCH₂CH₂OH F-48 —OCH₂COO(CH₂)₉OH F-49 —OCH₂COOCH₂CH₂OSO₂N(CH₃)₂ F-50 —OCH₂COOCH₂CH₂COCH₃ F-51 —OCH₂COOCH₂CH₂ON(CH₃)₂ F-52 —OCH₂COOCH₂CH₂N(CH₃)₂ F-53 —OCH₂COOCH₂CH₂N(OC₂H₅)C₂H₅ F-54 —OCH₂COOCH₂CH₂NHCOCH₃ F-55 —OCH₂COOCH₂CH₂N(CH₃)COCH₃ F-56 —OCH₂COOCH₂CH₂NHCOOCH₂CH₂OCH₃ F-57 —OCH₂COOCH₂CH₂NHCOSCH₂CH═CH₂ F-58 —OCH₂COOCH₂CH₂NHCONHC₂H₅ F-59 —OCH₂COOCH₂CH₂NHCON(CH₃)₂ F-60 —OCH₂COOCH₂CH₂NHCON(OCH₃)CH₃ F-61 —OCH₂COOCH₂CH₂NHCSNHCH₂CH₂Cl F-62 —OCH₂COOCH₂CH₂NHSO₂N(CH₃)₂ F-63 —OCH₂COOCH₂CH₂CN F-64 —OCH₂COOCH₂CH₂NO₂ F-65 —OCH₂COOCH₂CH₂SO₃H F-66

F-67 —OCH₂CONHCH₂CH₂SO₂N(CH₃)₂ F-68 —OCH₂COSCH₃ F-69 —OCH₂CONH₂ F-70 —OCH₂CONHCH₃ F-71 —OCH₂CON(CH₃)₂ F-72 —OCH₂CON(CH₃)CH₂C≡CH F-73 —OCH₂CON(OCH₃)CH₃ F-74 —OCH₂CONHOCH₃ F-75 —OCH₂CONHOCH₂CH═CH₂ F-76 —OCH₂COOCH₂COOCH₃ F-77 —OCH₂COSCH₂COOCH₃ F-78 —OCH₂CONHCH₂COOCH₃ F-79 —OCH₂CONHCH₂CON(CH₃)₂ F-80 —OCH₂CONHCH₂CN F-81 —OCH₂CONHCH₂C(═NH)NH₂ F-82 —OCH₂CONHSO₂CH₃ F-83

F-84 —OCH₂CONHN(CH₃)₂ F-85 —OCH₂CONHNHCOOC₂H₅ F-86 —OCH₂CONHNHCSNH(c)C₆H₁₁ F-87 —SCH₂CN F-88 —CH₂SCH₂COOCH₃ F-89 —CH₂SOCH₂COOCH₃ F-90 —CH₂SO₂CH₂COOCH₃ F-91 —NHCH₂COOCH₃ F-92 —NHCH₂CH₂N(CH₃)₂ F-93 —N(COCH₃)CH₂CH₂OH F-94 —CH₂OCH₂COOCH₃

Groups belonging to the G₀ group and the G group will be exemplified in Table G.

TABLE G No. Group G-1

G-2

G-3

G-4

G-5

G-6

G-7 —OCH₂CH═CH₂ G-8 —OCH₂C≡CH G-9 —OCH₂CH═CHCl G-10 —SCH═CHOCH₃ G-11 —SO₂CH═CHOCH₃ G-12 —OCH═CHCOCH₃ G-13 —OCH═CHCHO G-14 —OCH═CHCH═NCH₂CH═CH₂ G-15 —OCH═CHCH═NOCH₃ G-16 —OCH═CHCH═NN(CH₃)₂ G-17 —OCH═CHCN G-18 —OCH═CHC(═NH)NH₂ G-19 —OCH═CHCOOH G-20 —OCH₂C≡CCOOH G-21 —OCH═CHCOOCH₃ G-22 —OCH═CHCOOCH₂CH₂Cl G-23 —OCH═CHCOOCH₂CH═CH₂ G-24 —OCH═CHCOOCH₂C≡CH G-25

G-26

G-27 —OCH═CHCOOCH₂CH₂OCH₃ G-28 —OCH═CHCOOCH₂CH₂SCH₃ G-29 —OCH═CHCOOCH₂CH₂SOCH₃ G-30 —OCH═CHCOOCH₂CH₂SO₂CH₃ G-31 —OCH═CHCOOCH₂CH₂OH G-32 —OCH═CHCOOCH₂CH₂OSO₂N(CH₃)₂ G-33 —OCH═CHCOOCH₂CH₂COCH₃ G-34 —OCH═CHCOOCH₂CH₂ON(CH₃)₂ G-35 —OCH═CHCOOCH₂CH₂N(CH₃)₂ G-36 —OCH═CHCOOCH₂CH₂N(OC₂H₅)C₂H₅ G-37 —OCH═CHCOOCH₂CH₂NHCOCH₃ G-38 —OCH═CHCOOCH₂CH₂N(CH₃)COCH₃ G-39 —OCH═CHCOOCH₂CH₂NHCOOCH₂CH₂OCH₃ G-40 —OCH═CHCOOCH₂CH₂NHCOSCH₂CH═CH₂ G-41 —OCH═CHCOOCH₂CH₂NHCONHC₂H₅ G-42 —OCH═CHCOOCH₂CH₂NHCON(CH₃)₂ G-43 —OCH═CHCOOCH₂CH₂NHCON(OCH₃)CH₃ G-44 —OCH═CHCOOCH₂CH₂NHCSNHCH₂CH₂Cl G-45 —OCH═CHCOOCH₂CH₂NHSO₂N(CH₃)₂ G-46 —OCH═CHCOOCH₂CH₂C(═NH)NH₂ G-47 —OCH═CHCOOCH₂CH₂NO₂ G-48 —OCH═CHCOOCH₂CH₂SO₃H G-49

G-50 —OCH═CHCONHCH₂CH₂SO₂N(CH₃)₂ G-51 —OCH═CHCOSCH₃ G-52 —OCH═CHCON(CH₃)CH₂C≡CH G-53 —OCH═CHCON(OCH₃)CH₃ G-54 —OCH═CHCONHOCH₃ G-55 —OCH═CHCONHOCH₂CH═CH₂ G-56 —OCH═CHCONHCH₂COOCH₃ G-57 —OCH═CHCONHCH₂CON(CH₃)₂ G-58 —OCH═CHCONHSO₂CH₃ G-59

G-60 —OCH═CHCONHN(CH₃)₂ G-61 —OCH═CHCONHNHCOOC₂H₅ G-62 —OCH═CHCONHNHCSNH(c)C₆H₁₁ G-63

G-64

G-65 —OCH═CHCH₂OCH₃ G-66 —OCH═CHCH₂SCH₃ G-67 —OCH═CHCH₂SOCH₃ G-68 —OCH═CHCH₂SO₂CH₃ G-69 —OCH═CHCH₂OH G-70 —OCH═CHCH₂OCOCH₃ G-71 —OCH₂C≡CCH₂OH G-72 —OCH═CHCH₂ON(CH₃)₂ G-73 —OCH═CHCH₂N(CH₃)₂ G-74 —OCH═CHCH₂N(CH₂CH═CH₂)₂ G-75 —OCH═CHCH₂N(OH)CH₃ G-76 —OCH═CHCH₂NO₂ G-77 —OCH═CHCH₂SO₃H G-78 —SCH₂CH═CH₂ G-79 —SOCH₂CH═CH₂ G-80 —SO₂CH₂CH═CH₂ G-81 —SCH═CHCOOH G-82 —CH₂NHCH═CHCOOH G-83 —CH₂OCH₂CH═CH₂ G-84 —CH₂OCH═CHCOOH

Groups belonging to the H₀ group and the H group will be exemplified in Table H.

TABLE H No. Group H-1 —CH₂NHCN H-2 —N(COCH₃)CN H-3 —NHC(═NH)NHOH H-4 —NHC(═NH)N(CH₂CH═CH₂)CH₃ H-5 —C(═NH)NHCH₂CH═CH₂ H-6 —N═CHN(CH₃)₂ H-7 —N(CH₃)C(CH₃)═NOCH₂C≡CH H-8 —NHCONHCOCH₃ H-9 —NHCONHSO₂CH₃ H-10 —NHCOCN H-11 —NHCOCOOCH₃

Groups belonging to the I₀ group and the I group will be exemplified in Table I.

TABLE I No. Group I-1 —NHCOCH═CH₂ I-2 —NHCSCH═CH₂ I-3 —NHCOCF═CH₂ I-4 —NHCOC≡CH I-5 —NHCOCH₂OCH₃ I-6 —NHCOCH₂SCH₃ I-7 —NHCOCH₂COCH₃ I-8 —NHCOCH₂OH I-9 —NHCOCH₂ONH₂ I-10 —NHCOCH₂N(CH₃)CH₂C≡CH I-11 —NHCOCH₂NHCOCH₃ I-12 —NHCOCH₂COOCH₃ I-13 —NHCOCH₂CN I-14 —NHCOCH₂NO₂ I-15 —NHCOCH₂SO₃H I-16 —NHCOCH₂SO₂N(CH₃)₂ I-17 —NHCSCH₃ I-18 —NHCSCH₂N(CH₃)₂ I-19 —NHCOOCH₂CH₂OCH₃ I-20 —NHCOOCH₂CN I-21 —NHCOOCH₂CH₂NO₂ I-22 —NHCOOCH₂CH₂NHCOCH₃ I-23 —NH(CS)OCH₃ I-24 —NH(CO)SCH₃ I-24 —NHCONHCH₂CH₂OCH₃ I-25 —NHCSNHCH₃ I-26 —NHSO₂CH═CH₂ I-27 —NHSO₂CH₂CH═CH₂ I-28 —NHSO₂CH₂C≡CH I-29 —NHSO₂CH₂COCH₃ I-30 —NHSO₂CH₂CN I-31 —NHSO₂CH₂NO₂ I-32 —NHSO₂CH₂COOH I-33 —NHSO₂CH₂COOCH₃

Groups belonging to the J₀ group and the J group will be exemplified in Table J.

TABLE J No. Group J-1 —COCH═CH₂ J-2 —COC≡CH J-3 —COC≡CCF₃ J-4 —COCH₂SCH₃ J-5 —COCH₂OH J-6 —COCH₂N(CH₃)₂ J-7 —CSCH₃ J-8 —CSCF₃ J-9 —CH═NCH₃ J-10 —CH═NOCH₃ J-11 —COCN J-12 —COC(═NH)NH₂ J-13 —COCOOCH₃ J-14 —CH₂OCON(CH₃)₂

Groups belonging to the K₀ group and the K group will be exemplified in Table K.

TABLE K No. Group K-1 —CONHSO₂CH₃ K-2 —CONHOH K-3 —CONHOCH₃ K-4 —CONHOCH₂CH═CH₂ K-5 —CONHCH₂CH₂OH K-6 —CONHCH₂CH₂OCH₃ K-7 —CONHCH₂OCH₃ K-8 —CONHCH₂CH═CH₂ K-9 —CONHCH₂C≡CH K-10 —CONHCH₂CN K-11 —CONHCH₂COOH K-12 —CONHCH₂COOCH₃ K-13 —CONHCH₂CONH₂ K-14 —CONHCH₂CONHCH₃ K-15 —CONHCH₂CONH(CH₃)₂ K-16 —CONHCH(CH₂COOH)COOH K-17 —CONHCH(CH₂COOCH₃)COOCH₃

Groups belonging to the L₀ and the L group will be exemplified in Table L.

TABLE L No. Group L-1 —SO₂NHOH L-2 —SO₂NHOCH₃ L-3 —SO₂NHOCH₂CH═CH₂ L-4 —SO₂NHCH₂CH₂OCH₃ L-5 —SO₂NHCH₂CH═CH₂ L-6 —SO₂NHCH₂C≡CH L-7 —SO₂NHCH₂CN L-8 —SO₂NHCOCH₃ L-9 —SO₂NHCH₂COOH L-10 —SO₂NHCH₂COOCH₃ L-11 —SO₂NHCH₂CONH₂ L-12 —SO₂NHCH₂CONHCH₃ L-13 —SO₂NHCH₂CON(CH₃)₂ L-14 —SO₂NHCH(CH₂COOH)COOH L-15 —NHSO₂N(CH₃)₂

Groups belonging to the M₀ group and the M group will be exemplified in Table M.

TABLE M No. Group M-1 —N═C(—SCH₃)CH₃ M-2 —N═C(—OCH₃)OCH₃ M-3 —N═C(—SCH₃)OCH₃ M-4 —N═C(—SCH₃)SCH₃ M-5 —N═C(—SCH₃)NHCH₃ M-6 —N(CH₃)C(—SCH₃)═NCH₃ M-7 —N(CH₃)OCH₂CH═CH₂ M-8 —N(CH₂CH═CH₂)OCH₂CH═CH₂

Groups belonging to the N₀ group and the N group will be exemplified in Table N.

TABLE N No. Group N-1 —CH₂P(═O) (OH)₂ N-2 —CH₂P(═O)(OCH₃)₂ N-3 —CH₂P(═O)(OCH₃)—CH₃ N-4 —CH₂P(═O)(OCH₃)—CH(OH)CH₃ N-5 —CH₂P(═O)(OCH₃)—CH₂CH₂OH N-6 —CH₂P(═O)(OCH₃)—CH₂COOCH₃

Groups belonging to the aforementioned X₀ to Z₀ groups and X to Z groups will be exemplified in the following Table X to Table Z. When said groups have geometrical isomers, all of the geometrical isomers are included, and when said groups have tautomers, all of the tautomers are included.

Groups belonging to the X₀ group and the X group will be exemplified in Table X.

TABLE X No. Group X-1 —CH₃ X-2 —C₂H₅ X-3 —CF₃ X-4 —CH═CHCH₃ X-5 —CH₂CH═CH₂ X-6 —C≡CH X-7 —F X-8 —Cl X-9 —Br X-10 —NO₂ X-11 —CN X-12 —OCH₃ X-13 —SCH₃ X-14 —SOC₄H₉ X-15 —SO₂C₄H₉ X-16 —OCHF₂ X-17 —OCF₃ X-18 —OCF₂CHF₂ X-19 —SCF₃ X-20 —CH₂OCH₃ X-21 —COCH₃ X-22 —OCOCH₃ X-23 —COOH X-24 —COOCH₃ X-25 —CH═CHCOOH X-26 —N(CH₃)₂ X-27 —NHCOCH₃ X-28 —NHCOOCH₃ X-29 —CONH₂ X-30 —CON(CH₃)₂ X-31 —NHCON(CH₃)₂ X-32 —NHC(═NH)NH₂ X-33 —NHSO₂CF₃ X-34 —SO₂N(CH₃)₂

Groups belonging to the Y₀ group and the Y group will be exemplified in Table Y.

TABLE Y No. Group Y-1

Y-2

Y-3

Y-4

Y-5

Y-6

Y-7

Y-8

Y-9

Y-10

The A ring fused to the Z₀ group or the Z group will be exemplified in Table Z.

TABLE Z No. Group Z-1

Z-2

Z-3

Z-4

Z-5

Z-6

Z-7

Z-8

Z-9

Z-10

Q_(A0) and Q_(A) will be exemplified in Table Q.

TABLE Q No. Group Q-1 —OH Q-2

Q-3

Q-4

Q-5 —OCOCH₃ Q-6 —OSO₂N(CH₃)₂ Q-7 —NHCH₂CH═CH₂ Q-8 —NHCH₂C≡CH Q-9 —NHCH₂CH₂OCH₃ Q-10 —OCH₃ Q-11 —OCH₂CH₂(c)C₆H₁₁ Q-12 —OCH₂CH═CH₂ Q-13 —OCH₂C≡CH Q-14 —OCH₂COOH Q-15 —OCH₂COOCH₃ Q-16 —OCH₂CONH₂ Q-17 —OCH₂CN Q-18 —OCH₂CH₂OH Q-19 —OCH₂CH₂OCH₃ Q-20 —OCH₂CH₂N(CH₃)₂ Q-21 —OCH₂COCH₃ Q-22 —OCOC₆H₅ Q-23 —OCH₂C₆H₅ Q-24

Q-25

Q-26

T_(A0) and T_(A) will be exemplified in Table T.

TABLE T No. Group T-1 —H T-2 —CH₃ T-3 —CH₂CH₂(c)C₆H₁₁ T-4 —CH₂CH═CH₂ T-5 —CH₂C≡CH T-6 —CH₂C₆H₅ T-7 —CH₂COOH T-8 —CH₂COOCH₃ T-9 —CH₂CONH₂ T-10 —CH₂CN T-11 —CH₂CH₂OH T-12 —CH₂CH₂OCH₃ T-13 —CH₂CH₂N(CH₃)₂ T-14 —CH₂COCH₃ T-15 —CH₂CF₃ T-16 —Ph T-17

The present compound (I) includes, for example, a 2(1H)-pyridinone compound represented by the formula (I′):

wherein A, X_(α), Y_(α), p, q, Q_(α) and T_(α) are as defined above, and x represents a methine group or a nitrogen atom. In the 2(1H)-pyridinone compound (I′), when x is a methine group, the methine group has no substituent. Specifically, the 2(1H)-pyridinone compound (I′) includes the compound in which Q_(α) is an optionally substituted hydroxy group.

The present compound (II) includes, for example, a 2(1H)-pyridinone compound represented by the formula (II′):

wherein A, X_(A0), Y_(A0), p, q, Q_(A0) and T_(A0) are as defined above, and x represents a methine group or a nitrogen atom. In the 2(1H)-pyridinone compound (II′), when x is a methine group, the methine group has no substituent. Specifically, the 2(1H)-pyridinone compound (II′) includes the compound in which Q_(A0) is a hydroxyl group, an A₉′-O— group (wherein A₉′ is as defined above) or a M_(c)-O— group (wherein M_(c) is as defined above).

The present compound (III) includes, for example, a 2(1H)-pyridinone compound represented by the formula (III′):

wherein A, X_(A), Y_(A), p, q, Q_(A) and T_(A) are as defined above, and x represents a methine group or a nitrogen atom. In the 2(1H)-pyridinone compound (III′), when x is a methine group, the methine group has no substituent. Specifically, the 2(1H)-pyridinone compound (III′) includes the compound in which Q_(A) is a hydroxyl group, an A₉′-O— group (wherein A₉′ is as defined above) or a M_(c)-O— group (wherein M_(c) is as defined above). More specifically, in the 2(1H)-pyridinone compound (III′), when Q_(A) is a hydroxyl group, A₉′-O— group (wherein A₉′ is as defined above) or a M_(c)-O— group (wherein M_(c) is as defined above), X_(A) represents a substituent belonging to the F group, the I group or the K group.

In the present compound (IV), for example, q_(a) is a r_(a)-O— group (wherein r_(a) is as defined above).

In the present compound (V), for example, q_(a) is a r_(a)-O— group (wherein r_(a) is as defined above).

In the present compound (VI), for example, q_(a) is a r_(a)-O— group (wherein r_(a) is as defined above).

In the present compound (VII), for example, q_(a)′ is a r_(a)′-O— group (wherein r_(a)′ is as defined above)

In the present compound (VIII), for example, q_(a) is a r_(a)-O— group (wherein r_(a) is as defined above).

In the present compound (IX), for example, q_(a)″ is a hydroxy group or a C1-C10 alkoxy group.

The present compound (X) includes, for example, a 2(1H)-pyridinone compound represented by the formula (X′):

wherein Z₁, Y₁, n, a and b are as defined above.

The present compound (XI) includes, for example, a 2(1H)-pyridinone compound represented by the formula (XI′):

wherein X₁′, a and b are as defined above.

The present compound (XII) includes, for example, a 2(1H)-quinolinone compound represented by the formula (XII′):

wherein X₁′, a and b are as defined above.

Typical examples of the present compound (I) include: a 2(1H)-pyridinone compound represented by the formula (XV):

a 2(1H)-pyridinone compound represented by the formula (XVI):

a 2(1H)-pyridinone compound represented by the formula (XVII):

a 2(1H)-pyridinone compound represented by the formula (XVIII):

a 2(1H)-pyridinone compound represented by the formula (XIX):

a 2(1H)-pyridinone compound represented by the formula (XX):

a 2(1H)-pyridinone compound represented by the formula (XXI):

a 2(1H)-pyridinone compound represented by the formula (XXII):

a 2(1H)-pyridinone compound represented by the formula (XXIII):

a 2(1H)-pyridinone compound represented by the formula (XXIV):

a 2(1H)-pyridinone compound represented by the formula (XXV):

a 2(1H)-pyridinone compound represented by the formula (XXVI):

a 2(1H)-pyridinone compound represented by the formula (XXVII):

a 2(1H)-pyridinone compound represented by the formula (XXVIII):

a 2(1H)-pyridinone compound represented by the formula (XXIX):

a 2(1H)-pyridinone compound represented by the formula (XXX):

a 2(1H)-quinolinone compound represented by the formula (XXXI):

a 2(1H)-quinolinone compound represented by the formula (XXXII):

a 2(1H)-quinolinone compound represented by the formula (XXXIII):

a 2(1H)-quinolinone compound represented by the formula (XXXIV):

a 2(1H)-quinolinone compound represented by the formula (XXXV):

a 2(1H)-quinolinone compound represented by the formula (XXXVI):

a 2(1H)-quinolinone compound represented by the formula (XXXVII):

a 2(1H)-quinolinone compound represented by the formula (XXXVIII):

and the like.

The present compounds are novel compounds. Although WO 00/20371, JP2002-371078 and WO 92/18483 disclose compounds having a certain conceptional skeleton, they do not describe concretely a compound having a structure similar to that of the present compounds. In addition, in these publications, there is no description regarding a suppressing effect on transcription of a Type I collagen gene in tissues and then a suppressing effect on accumulation of collagen.

[Process A for Producing the Present Compound]

The present compound (I) can be produced by reacting a compound represented by the formula (a) (wherein A, X_(α), Y_(α), p and q are as defined above) with a compound represented by the formula (α′) (wherein Q_(α), T_(α), K_(α) and L_(α) are as defined above) (see Russian J. General Chem. (2001), 71, 1257).

The present compound (II) can be produced by reacting a compound represented by the formula (A0) (wherein A, X_(A0), Y_(A0), p and q are as defined above) with a compound represented by the formula (A0′) (wherein Q_(A0), T_(A0), K_(A0) and L_(A0) are as defined above) as described above.

The present compound (III) can be produced by reacting a compound represented by the formula (A) (wherein A, X_(A), Y_(A), P and q are as defined above) with a compound represented by the formula (A′) (wherein Q_(A), T_(A), K_(A) and L_(A) are as defined above) as described above.

The present compound (IV) can be produced by reacting a compound represented by the formula (a) (wherein A, X_(a), Y_(a), p and q are as defined above) with a compound represented by the formula (a′) (wherein Q_(a), T_(a), K_(a) and L_(a) are as defined above) as described above.

Although a part of compounds represented by the formula (a) is known, for example, by EP330645, benzaldehyde derivatives represented by the aforementioned formulas (XXXIX-1), (XXXIX-2), (XXXIX-3) and (XXXIX-4) (hereinafter, referred to as the present benzaldehyde derivative in some cases) and 6-formyl-2-[(2-methoxyethyl)aminocarbonyl]pyridine (hereinafter, referred to as the present pyridinecarbaldehyde derivative in some cases) have never been reported, and they are novel substances.

The present benzaldehyde derivative can be produced, for example, by reacting a compound represented by the formula (XXXIX-a):

with glycine methyl ester. In the reaction, the reaction temperature is usually room temperature to the reflux temperature of a solvent, and the reaction time is usually instant to about 24 hours. The reaction is usually performed in the presence of a base. Examples of a base used include organic bases such as pyridine, triethylamine, N,N-dimethylaniline, tributylamine, N-methylmorpholine and the like, and inorganic bases such as sodium hydroxide, potassium hydroxide, potassium carbonate and the like. In the reaction, 1 to 2 mol of glycine methyl ester and 1 to 7 mol of a base are usually per 1 mol of the compound (XXXIX-a). In the reaction, a solvent is not necessarily required, but the reaction is usually performed in the presence of a solvent. Examples of a solvent which may be used in the reaction include aliphatic hydrocarbons such as hexane, petroleum ether and the like, aromatic hydrocarbons such as benzene, toluene and the like, halogenated hydrocarbons such as chloroform, dichloroethane and the like, ethers such as diethyl ether, dioxane, tetrahydrofuran and the like, ketones such as acetone, methyl ethyl ketone and the like, esters such as ethyl acetate, diethyl carbonate and the like, nitrites such as acetonitrile, isobutylnitrile and the like, amides such as formamide, N,N-dimethylformamide and the like, sulfur compounds such as dimethyl sulfoxide and the like, and a mixture thereof. After completion of the reaction, the reaction solution can be subjected to conventional posttreatment, for example, concentration of an organic layer under reduced pressure after organic solvent extraction and washing with water, and then, if necessary, purification by chromatography, recrystallization or the like, to obtain the objective compound of the present invention.

The present benzaldehyde derivative can be also produced, for example, by oxidizing a compound represented by the formula (XXXIX-b):

in the presence of a base such as triethylamine in dichloromethane using dimethyl sulfoxide which has been activated with oxalyl chloride (SYNTHESIS (1981), 165).

A compound represented by the formula (XXXIX-b) can be produced, for example, by reacting a compound represented by the formula (XXXIX-c):

with methoxyacetyl chloride. The reaction of the compound (XXXIX-c) with methoxyacetyl chloride can be performed in a similar way to the reaction of the compound (XXXIX-a) with glycine methyl ester.

The present benzaldehyde derivative can be also produced, for example, by reacting a compound represented by the formula (XXXIX-d):

with 2-methoxyethylamine. The reaction of the compound (XXXIX-d) and 2-methoxyethylamine can be performed in a similar way to the reaction of the compound (XXXIX-a) and glycine methyl ester.

The compound (XXXIX-d) is known by an article, for example, J. Medicinal Chem. (2001), 44, 362.

The present pyridinecarbaldehyde derivative can be produced by reacting a compound represented by the formula (XXXIX-e):

with 2-methoxyethylamine. The reaction of the compound (XXXIX-e) and 2-methoxyethylamine can be performed in a similar way to the reaction of the compound (XXXIX-a) with glycine methyl ester. The compound (XXXIX-e) can be produced by reacting 2-carboxy-6-formylpyridine with a chlorinating agent such as phosphoryl chloride, thionyl chloride or phosphorus trichloride. In the reaction, the reaction temperature is usually room temperature to the reflux temperature of as solvent, and the reaction time is usually instant to about 24 hours. In the reaction, 1 to 10 mol of a chlorinating agent is usually used per 1 mol of 2-carboxy-6-formylpyridine. In the reaction, a solvent is not necessarily required, but the reaction is usually performed in the presence of a solvent. Examples of a solvent which may be used in the reaction include aliphatic hydrocarbons such as hexane, petroleum ether and the like, aromatic hydrocarbons such as benzene, toluene and the like, halogenated hydrocarbons such as chloroform, dichloroethane and the like, ethers such as diethyl ether, dioxane, tetrahydrofuran and the like, and a mixture thereof. After completion of the reaction, volatile substances can be distilled off under reduced pressure to obtain the compound (XXXIX-e). 2-Carboxy-6-formylpyridine is known by an article, for example, Bioorg. Medicinal Chem. Letters (2003) 13, 609.

Among the present compounds (IV), a cinnamoyl compound represented by the formulas (LIX-1), (LIX-2), (LIX-3), (LIX-4) and (LIX-5) can be produced by reacting the present benzaldehyde derivative or the present pyridinecarbaldehyde derivative with the compound (LIX).

[Process B for Producing the Present Compound]

Among the present compounds, a cinnamoyl compound represented by the formula (LX″) can be produced by reacting a cinnamoyl compound represented by the formula (LX) with a compound represented by the formula (LX′).

An example of the reaction comprises a reaction of the compound (LX) with the compound (LX′) in the presence of a base.

The reaction of the compound (LX) with the compound (LX′) in the presence of a base is usually performed in a solvent. Examples of a solvent used in the reaction include acid amides such as N,N-dimethylformamide, N,N-dimethylacetamide and the like, sulfoxides such as dimethyl sulfoxide and the like, phosphoric acid amide compound such as hexamethylphosphoramide and the like, and ketones such as acetone, methyl ethyl ketone and the like.

Examples of a base used in the reaction include alkali metal hydrides such as sodium hydride, potassium hydride and the like, carbonates of an alkali metal such as potassium carbonate and the like, and silver oxide and the like.

Examples of the compound (LX′) include alkylsulfonic acid esters such as methyl methanesulfonate and the like, arylsulfonic acid esters such as methyl p-toluenesulfonate, 2-methoxyethyl p-toluenesulfonate and the like, sulfate esters such as dimethyl sulfate and the like, and halides such as methyl iodide, 2-chloroethyldimethylamine, allyl bromide, propargyl bromide, methyl bromoacetate, bromoacetonitrile, 2-bromoethanol, benzyl bromide, bromoacetone and the like.

In the reaction, 1 to 2 mol of a base and 1 to 2 mol of the compound (LX′) are usually used per 1 mol of the compound (LX).

The reaction temperature is usually 0° C. to 100° C., and the reaction time is usually 1 hour to 200 hours.

After completion of the reaction, the reaction mixture can be subjected to posttreatment such as drying, concentration and the like of an organic layer after extraction with an organic solvent, to isolate the cinnamoyl compound (LX″). The isolated compound (LX″) can be also further purified by chromatography, recrystallization or the like.

[Process C for Producing the Present Compound]

Among the present compounds, a cinnamoyl compound represented by the formula (LXI′) can be produced by hydrolyzing a cinnamoyl compound represented by the formula (LXI).

Hydrolysis of a cinnamoyl compound (LXI) is performed in the presence of an acid or a base usually in a solvent. Examples of a solvent used in the reaction include water, alcohols such as methanol, ethanol and the like, ketones such as acetone, methyl ethyl ketone and the like, and a mixture thereof.

Examples of an acid used in the reaction include inorganic acids such as hydrochloric acid, sulfuric acid, hydrobromic acid and the like, and organic acids such as p-toluenesulfonic acid and the like.

Examples of a base used in the reaction include alkali metal hydroxides such as sodium hydroxide, potassium hydroxide and the like, and carbonates of an alkali metal such as sodium carbonate, potassium carbonate and the like.

In the reaction, 1 to 10 mol of a base is usually used per 1 mol of the compound (LXI).

The reaction temperature is usually 0° C. to the reflux temperature of a solvent, and the reaction time is usually 1 hour to 200 hours.

After completion of the reaction, the reaction mixture can be subjected to posttreatment such as drying, concentration and the like of an organic layer after extraction with an organic solvent, to isolate the cinnamoyl compound (LXI′). The isolated compound (LXI′) can be also further purified by chromatography, recrystallization or the like.

[Process D for Producing the Present Compound]

Among the present compounds, a cinnamoyl compound represented by the formula (LXII″) can be produced by reacting a cinnamoyl compound represented by the formula (LXII) with a compound represented by the formula (LXII′).

An example of the reaction comprises a reaction of the compound (LXII) with the compound (LXII′) in the presence of a base.

The reaction of the compound (LXII) with the compound (LXII′) in the presence of a base can be performed in a similar way to the reaction of the compound (LX) with the compound (LX′).

Examples of the compound (LXII′) include alkylsulfonic acid esters such as methyl methanesulfonate and the like, arylsulfonic acid esters such as p-toluenesulfonic acid methyl ester, p-toluenesulfonic acid 2-methoxyethyl ester and the like, sulfate esters such as dimethyl sulfate and the like, and halides such as methyl iodide, 2-chloroethyldimethylamine, allyl bromide, propargyl bromide, methyl bromacetate, bromoacetonitrile, 2-bromoethanol, benzyl bromide, bromoacetone and the like.

[Process E for Producing the Present Compound]

Among the present compounds, a cinnamoyl compound represented by the formula (LXIII″) can be produced by reacting a cinnamoyl compound represented by the formula (LXIII) with a compound represented by the formula (LXIII′), 1,3-propanesultone or 1,4-butanesultone.

An example of the reaction comprises a reaction of the compound (LXIII), with the compound (LXIII′) in which V′ is a leaving group, 1,3-propanesultone or 1,4-butanesultone in the presence of a base.

The reaction of the compound (LXIII), with the compound (LXIII′) in which V′ is a leaving group, 1,3-propanesultone or 1,4-butanesultone in the presence of a base can be performed in a similar way to the reaction of the compound (LX) with the compound (LX′).

Examples of the compound (LXIII′) in which V′ is a leaving group include alkylsulfonic acid esters such as 2-methoxyethyl methanesulfonate and the like, arylsulfonic acid esters such as p-toluenesulfonic acid 2-methoxyethyl ester and the like, and halides such as 2-chloroethyldimethylamine, allyl bromide, propargyl bromide, methyl bromoacetate, bromoacetonitrile, 2-bromoethanol, bromoacetone and the like.

In addition, an example of the reaction comprises a dehydration reaction of the compound (LXIII) and the compound (LXIII′) in which V′ is a hydroxyl group in the presence of triphenylphosphine and azodicarboxylic acid ester.

The reaction is performed usually in a solvent. Examples of a solvent used in the reaction include ethers such as tetrahydrofuran and the like. Examples of azodicarboxylic acid ester include diethyl azodicarboxylate.

In the reaction, 1 to 2 mol of triphenylphosphine, 1 to 2 mol of azodicarboxylic acid ester, and 1 to 2 mol of the compound (LXIII′) are usually used per 1 mol of the compound (LXIII).

The reaction temperature is usually 0° C. to room temperature, and the reaction time is usually 1 hour to 200 hours.

After completion of the reaction, the reaction mixture can be subjected to posttreatment such as drying, concentration and the like of an organic layer after extraction with an organic solvent, to isolate the cinnamoyl compound (LXIII″). The isolated compound (LXIII″) can be also further purified by chromatography, recrystallization or the like.

In Table 1, the benzaldehyde derivatives (XXXIX-1), (XXXIX-2), (XXXIX-3) and (XXXIX-4) represented by compound numbers (a) to (p) and (r) to (x) are exemplified, and a pyridinecarbaldehyde derivative represented by the compound number (q) is shown.

TABLE 1 (XXXIX-1)

(XXXIX-2)

(XXXIX-3)

(XXXIX-4) Compound No. X_(b), X_(b)′, X_(b)″ or X_(b)′″ (a) 3-NHCOCH₂OCH₃ (b) 3-CONHCH₂COOCH₃ (c) 4-CONHCH₂COOCH₃ (d) 3-CONHCH₂CH₂OCH₃ (e) 3-CH═CHCN (f) 3-OCH₂CH₂SCH₃ (g) 3-CH₂OCH₂CH₂OH (h) 3-NHCOOCH₂CH₂OCH₃ (i) 3-NHCONHCH₂CH₂OCH₃ (j) 3-CONHSO₂CH₃ (k) 3-CONHCH₂CN (l) 3-CH═CF₂ (m) 3-CH₂CH₂CN (n) 3-OCH₂CONH₂ (o) 3-OCH₂COCH₃ (p) 3-CONHCH(CO₂CH₃)CH₂CO₂CH₃ Compound No. Pyridinecarbaldehyde compound (q)

Compound No. X_(b), X_(b)′, X_(b)″ or X_(b)′″ (r) 3-SO₂NHCH₂CH₂OCH₃ (s) 3-CONHOCH₃ (t) 3-CONHOCH₂CH═CH₂ (u) 3-CH₂SCH₂COOCH₃ (v)

(w) 3-NHCOCOOCH₃ (x) 3-CH₂P(═O)(OCH₃)₂

Among the present compounds (IV), the present compounds (IVa) represented by the compound numbers (1a) to (116a) are exemplified in Table 2.

TABLE 2a the present compound (IVa): (IVa)

In Table 2a, in the compound numbers (1a) to (98a), (100a) to (104a) and (106a) to (116a), A represents a benzene ring. Compound No. X_(a) and Y_(a) r_(a) t_(a) (1a) 3-CH═CHCN —H —H (2a) 3-OCH₂CH₂SCH₃ —H —H (3a) 3-OCH₂CH=CH₂ —H —H (4a) 2-OCH₂C≡CH —H —H (5a) 3-OCH₂C≡CH —H —H (6a) 4-OCH₂C≡CH —H —H (7a) 3-OCH₂COOCH₃ —H —H (8a) 3-OCH₃,4-OCH₂COOCH₃ —H —H (9a) 3-OCH₂COOH —H —H (10a) 3-OCH₂CN —H —H (11a) 3-OCH₂CN —H —CH₃ (12a) 3-OCH₂CN —CH₃ —CH₃ (13a) 4-OCH₂CN —H —H (14a) 3-CH₃,4-OCH₂CN —H —H (15a) 3-NO₂,4-OCH₂CN —H —H (16a) 3-F,4-OCH₂CN,5-OCH₃ —H —H (17a) 3-NHCOCH=CH₂ —H —H (18a) 3-NHCOCH₂OCH₃ —H —H (19a) 3-NHCOCH₂OCH₃ —H —CH₃ (20a) 4-NHCOCH₂OCH₃ —H —H (21a) 3-NHCOOCH₂CH₂OCH₃ —H —H (22a) 3-NHCONH₂CH₂OCH₃ —H —H (23a) 3-NHSO₂CH₂COOCH₃ —H —H (24a) 3-NHSO₂CH₂COOH —H —H (25a) 3-NHCOCH₂CN —H —H (26a) 3-CONHSO₂CH₃ —H —H (27a) 3-CONHCH₂CH₂OH —H —H (28a) 3-CONHCH₂COOCH₃ —H —H (29a) 3-CONHCH₂COOCH₃ —H —CH₃ (30a) 4-CONHCH₂COOCH₃ —H —H (31a) 3-CONHCH₂CH₂OCH₃ —H —H (32a) 3-CONHCH₂CH₂OCH₃ —H —CH₃ (33a) 4-CONHCH₂CH₂OCH₃ —H —H (34a) 3-CONHCH₂COOH —H —H (35a) 3-CONHCH₂CN —H —H (36a) 3-OCH₂COOCH₃ —H —CH₃ (37a) 3-OCH₂COOH —H —CH₃ (38a) 3-OCH₂CON(CH₃)₂ —H —CH₃ (39a) 3-OCH₂CH₂CH₂N(CH₃)₂ —H —H (40a) 3-OCH₂CH₂OH —H —CH₃ (41a) 3-OCH₂CH₂OH —CH₃ —CH₃ (42a) 3-NHCOOCH₂CH₂OCH₃ —H —CH₃ (43a) 3-CH=CF₂ —H —H (44a) 3-CH₂CH₂CN —H —H (45a) 3-OCH₂CH₂SCH₃ —H —CH₃ (46a) 3-OCH₂CH₂SOCH₃ —H —CH₃ (47a) 3-OCH₂CH₂SO₂CH₃ —H —CH₃ (48a) 3-OCH₂CH₂OH —H —H (49a) 3-CH₂OCH₂CH₂OH —H —CH₃ (50a) 3-OCH₂OCH₂CH₂OH —H —CH₃ (51a) 3-OCH₂CH₂OCH₃ —H —CH₃ (52a) 3-OCH₂CH₂NH₂ —H —CH₃ (53a) 3-OCH₂CH₂NHCOCH₃ —H —CH₃ (54a) 3-OCH₂CH₂NHCOOC(CH₃)₃ —H —CH₃ (55a) 3-OCH₂CH₂N(CH₃)₂ —H —H (56a) 3-OCH₂CH₂N(CH₃)₂ —H —CH₃ (57a) 3-OCH₂CH₂CH₂N(CH₃)₂ —H —CH₃ (58a) 3-OCH₂CH₂SO₃H —H —CH₃ (59a) 3-OCH₂CH₂CH₂SO₃Na —Na —H (60a) 3-OCH₂COOCH₃ —CH₃ —CH₃ (61a) 3-OCH₂COO(CH₂)₉—OH —H —CH₃ (62a) 4-OCH₂COOCH₃ —H —H (63a) 3-OCH₂COOH.pyridine —H —H (64a) 3-OCH₂COOH —CH₃ —CH₃ (65a) 4-OCH₂COOH —H —H (66a) 3-OCH₂CONH₂ —H —H (67a) 3-OCH₂CONH₂ —H —CH₃ (68a) 3-OCH₂CONH₂ —CH₃ —CH₃ (69a) 3-OCH₂CON(CH₃)₂ —H —H (70a) 3-OCH₂CON(CH₃)₂ —CH₃ —CH₃ (71a) 3-Br,4-OCH₂COOCH₃ —H —H (72a) 3-CH₃,4-OCH₂COOCH₃ —H —H (73a) 3-CH₃,4-OCH₂COOCH₃ —H —CH₃ (74a) 3-NHCOCH₃,4-OCH₂CN —H —H (75a) 3-OCH₂COCH₃ —H —CH₃ (76a) 3-CH₂SCH₂COOCH₃ —H —CH₃ (77a) 3-CH₂SOCH₂COOCH₃ —H —CH₃ (78a) 3-CH₂SO₂CH₂COOCH₃ —H —CH₃ (79a) 3-NHCOCH₂OCH₃ —CH₃ —CH₃ (80a) 3-NHCOOCH₂CH₂OCH₃ —CH₃ —CH₃ (81a) 3-NHSO₂CH₂CH═CH₂ —H —CH₃ (82a) 3-NHCH₂CH₂N(CH₃)₂ —H —H (83a) 3-CONHCH₂COOCH₃ —CH₃ —CH₃ (84a) 4-CONHCH₂COOCH₃ —H —CH₃ (85a) 4-CONHCH₂COOCH₃ —CH₃ —CH₃ (86a) 3-CONHCH₂COOH —H —CH₃ (87a) 3-CONHCH₂COOH —CH₃ —CH₃ (88a) 4-CONHCH₂COOH —H —H (89a) 4-CONHCH₂COOH —H —CH₃ (90a) 4-CONHCH₂COOH —CH₃ —CH₃ (91a) 3-CONHCH₂CONH₂ —H —H (92a) 3-CONHCH₂CONH₂ —H —CH₃ (93a) 3-CONHCH₂CONH₂ —CH₃ —CH₃ (94a) 3-CONHCH(CO₂CH₃) —H —H —CH₂CO₂CH₃ (95a) 3-CONHCH(CO₂H) —H —H —CH₂CO₂H (96a) 3-CONHCH₂CH₂OH —H —CH₃ (97a) 3-CONHCH₂CH₂OH —CH₃ —CH₃ (98a) 3-CONHCH₂CH₂OCH₃ —CH₃ —CH₃ (99a)

(100a) 3-CONHSO₂CH₃ —H —CH₃ (101a) 3-SO₂NHCH₂CH₂OCH₃ —H —H (102a) 3-SO₂NHCH₂CH₂OCH₃ —H —CH₃ (103a) 3-CONHOCH₃ —H —CH₃ (104a) 3-CONHOCH₂CH═CH₂ —H —CH₃ (105a)

(106a) 3-CH₂CH₂CN —H —CH₃ (107a)

—H —CH₃ (108a) 3-CH═CHCN —H —CH₃ (109a) 3-C═CC(CH₃)₂OH —H —CH₃ (110a) 3-CH═CHCOOCH₃ —H —CH₃ (111a) 3-OCH₂CH═CH₂ —H —CH₃ (112a) 3-NHCOCOOCH₃ —H —CH₃ (113a) 3-CH═NOCH₃ —H —CH₃ (114a) 3-NHCSNHCH3 —H —CH₃ (115a) 3-N═C(—SCH₃)NHCH₃ —H —CH₃ (116a) 3-CH₂P(═O)(OCH₃)₂ —H —CH₃

Among the present compound (IV), the present compounds (IVb) represented by the compound numbers (1b) to (14b) are exemplified in Table 2b.

TABLE 2b The present compound (IVb): (IVb)

Compound No. X_(a) q_(a) (1b) —OCH₂COOCH₃ —OCH₂CH═CH₂ (2b) —OCH₂COOCH₃ —OCH₂C≡CH (3b) —OCH₂COOCH₃ —OCH₂COOCH₃ (4b) —OCH₂COOH —OCH₂COOH (5b) —OCH₂CONH₂ —OCH₂CONH₂ (6b) —OCH₂COOCH₃ —OCH₂CN (7b) —OCH₂COOH —OCH₂CH₂OH (8b) —OCH₂COOCH₃ —OCH₂Ph (9b) —OCH₂COOH —OCH₂Ph (10b) —OCH₂COOCH₃ —OCH₂CH₂N(CH₃)₂ (11b) —OCH₂CH₂CH₂OH

(12b)

(13b) —OCH₂COOCH₃ —NHCH₂C═CH (14b) —OCH₂CONHCH₂CH₂OCH₃ —NHCH₂CH₂OCH₃

Among the present compounds (IV), the present compounds (IVc) represented by the compound numbers (1c) to (11c) are exemplified in Table 2c.

TABLE 2c The present compound (IVc): (IVc)

Compound No. t_(a) (1c) —CH₂CH═CH₂ (2c) —CH₂C≡CH (3c) —CH₂COOCH₃ (4c) —CH₂COOH (5c) —CH₂CONH₂ (6c) —CH₂CN (7c) —CH₂COCH₃ (8c) —CH₂CH₂OCH₃ (9c) —CH₂Ph (10c) —Ph (11c)

Among the present compounds (IV), the present compounds (IVd) represented by the compound numbers (1d) to (3d) are exemplified in Table 2d.

TABLE 2d The present compound (IVd): (IVd)

Com- pound No. Compound (1d)

(2d)

(3d)

Among the present compounds (IV), the present compounds (IVe) represented by the compound numbers (1e) to (116e) are exemplified in Table 2e.

TABLE 2e The present compound (IVe): (IVe)

In Table 2e, in the compound numbers (1e) to (98e), (100e) to (104e) and (106e) to (116e), A represents a benzene ring. Compound No. X_(a) and Y_(a) r_(a) t_(a) (1e) 3-CH═CHCN —H —H (2e) 3-OCH₂CH₂SCH₃ —H —H (3e) 3-OCH₂CH═CH₂ —H —H (4e) 2-OCH₂C≡CH —H —H (5e) 3-OCH₂C≡CH —H —H (6e) 4-OCH₂C≡CH —H —H (7e) 3-OCH₂COOCH₃ —H —H (8e) 3-OCH₃, 4-OCH₂COOCH₃ —H —H (9e) 3-OCH₂COOH —H —H (10e) 3-OCH₂CN —H —H (11e) 3-OCH₂CN —H —CH₃ (12e) 3-OCH₂CN —CH₃ —CH₃ (13e) 4-OCH₂CN —H —H (14e) 3-CH₃,4-OCH₂CN —H —H (15e) 3-NO₂,4-OCH₂CN —H —H (16e) 3-F,4-OCH₂CN,5-OCH₃ —H —H (17e) 3-NHCOCH═CH₂ —H —H (18e) 3-NHCOCH₂OCH₃ —H —H (19e) 3-NHCOCH₂OCH₃ —H —CH₃ (20e) 4-NHCOCH₂OCH₃ —H —H (21e) 3-NHCOOCH₂CH₂OCH₃ —H —H (22e) 3-NHCONHCH₂CH₂OCH₃ —H —H (23e) 3-NHSO₂CH₂COOH₃ —H —H (24e) 3-NHSO₂CH₂COOH —H —H (25e) 3-NHCOCH₂CN —H —H (26e) 3-CONHSO₂CH₃ —H —H (27e) 3-CONHCH₂CH₂OH —H —H (28e) 3-CONHCH₂COOCH₃ —H —H (29e) 4-CONHCH₂COOCH₃ —H —H (30e) 3-CONHCH₂CH₂OCH₃ —H —H (31e) 4-CONHCH₂CH₂OCH₃ —H —H (32e) 3-CONHCH₂COOH —H —H (33e) 3-CONHCH₂CN —H —H (34e) 3-CONHCH₂COOCH₃ —H —CH₃ (35e) 3-CONHCH₂CH₂OCH₃ —H —CH₃ (36e) 3-OCH₂COOCH₃ —H —CH₃ (37e) 3-OCH₂COOH —H —CH₃ (38e) 3-OCH₂CON(CH₃)₂ —H —CH₃ (39e) 3-OCH₂CH₂CH₂N(CH₃)₂ —H —H (40e) 3-OCH₂CH₂OH —H —CH₃ (41e) 3-OCH₂CH₂OH —CH₃ —CH₃ (42e) 3-NHCOOCH₂CH₂OCH₃ —H —CH₃ (43e) 3-CH═CF₂ —H —H (44e) 3-CH₂CH₂CN —H —H (45e) 3-OCH₂CH₂SCH₃ —H —CH₃ (46e) 3-OCH₂CH₂SOCH₃ —H —CH₃ (47e) 3-OCH₂CH₂SO₂CH₃ —H —CH₃ (48e) 3-OCH₂CH₂OH —H —H (49e) 3-CH₂OCH₂CH₂OH —H —CH₃ (50e) 3-OCH₂CH₂CH₂OH —H —CH₃ (51e) 3-OCH₂CH₂OCH₃ —H —CH₃ (52e) 3-OCH₂CH₂NH₂ —H —CH₃ (53e) 3-OCH₂CH₂NHCOCH₃ —H —CH₃ (54e) 3-OCH₂CH₂NHOOOC(CH₃)₃ —H —CH₃ (55e) 3-OCH₂CH₂N(CH₃)₂ —H —H (56e) 3-OCH₂CH₂N(CH₃)₂ —H —CH₃ (57e) 3-OCH₂CH₂CH₂N(CH₃)₂ —H —CH₃ (58e) 3-OCH₂CH₂SO₃H —H —CH₃ (59e) 3-OCH₂CH₂CH₂SO₃Na —Na —H (60e) 3-OCH₂COOCH₃ —CH₃ —CH₃ (61a) 3-OCH₂COO(CH₂)₉—OH —H —CH₃ (62e) 4-OCH₂COOCH₃ —H —H (63a) 3-OCH₂COOH.pyridine —H —H (64e) 3-OCH₂COOH —CH₃ —CH₃ (65e) 4-OCH₂COOH —H —H (66e) 3-OCH₂CONH₂ —H —H (67e) 3-OCH₂CONH₂ —H —CH₃ (68e) 3-OCH₂CONH₂ —CH₃ —CH₃ (69e) 3-OCH₂CON(CH₃)₂ —H —H (70e) 3-OCH₂CON(CH₃)₂ —CH₃ —CH₃ (71e) 3-Br,4-OCH₂COOCH₃ —H —H (72e) 3-CH₃,4-OCH₂COOCH₃ —H —H (73e) 3-CH₃,4-OCH₂COOCH₃ —H —CH₃ (74e) 3-NHCOCH₃,4-OCH₂CN —H —H (75e) 3-OCH₂COCH₃ —H —CH₃ (76e) 3-CH₂SCH₂COOCH₃ —H —CH₃ (77e) 3-CH₂SOCH₂COOCH₃ —H —CH₃ (78e) 3-CH₂SO₂CH₂COOCH₃ —H —CH₃ (79e) 3-NHCOCH₂OCH₃ —CH₃ —CH₃ (80e) 3-NHCOOCH₂CH₂OCH₃ —CH₃ —CH₃ (81e) 3-NHSO₂CH₂CH═CH₂ —H —CH₃ (82e) 3-NHCH₂CH₂N(CH₃)₂ —H —H (83e) 3-CONHCH₂COOCH₃ —CH₃ —CH₃ (84e) 4-CONHCH₂COOCH₃ —H —CH₃ (85e) 4-CONHCH₂COOCH₃ —CH₃ —CH₃ (86e) 3-CONHCH₂COOH —H —CH₃ (87e) 3-CONHCH₂COOH —CH₃ —CH₃ (88e) 4-CONHCH₂COOH —H —H (89e) 4-CONHCH₂COOH —H —CH₃ (90e) 4-CONHCH₂COOH —CH₃ —CH₃ (91e) 3-CONHCH₂CONH₂ —H —H (92e) 3-CONHCH₂CONH₂ —H —CH₃ (93e) 3-CONHCH₂CONH₂ —CH₃ —CH₃ (94e) 3-CONHCH(CO₂CH₃) —H —H —CH₂CO₂CH₃ (95e) 3-CONHCH(CO₂H) —H —H —CH₂CO₂H (96e) 3-CONHCH₂CH₂OH —H —CH₃ (97e) 3-CONHCH₂CH₂OH —CH₃ —CH₃ (98e) 3-CONHCH₂CH₂OCH₃ —CH₃ —CH₃ (99e)

(100e) 3-CONHSO₂CH₃ —H —CH₃ (101e) 3-SO₂NHCH₂CH₂OCH₃ —H —H (102e) 3-SO₂NHCH₂CH₂OCH₃ —H —CH₃ (103e) 3-CONHOCH₃ —H —CH₃ (104e) 3-CONHOCH₂CH═CH₂ —H —CH₃ (105e)

(106e) 3-CH₂CH₂CN —H —CH₃ (107e)

—H —CH₃ (108e) 3-CH═CHCN —H —CH₃ (109e) 3-C≡CC(CH₃)₂OH —H —CH₃ (110e) 3-CH═CHCOOCH₃ —H —CH₃ (111e) 3-OCH₂CH═CH₂ —H —CH₃ (112e) 3-NHCOCOOCH₃ —H —CH₃ (113e) 3-CH═NOCH₃ —H —CH₃ (114e) 3-NHCSNHCH3 —H —CH₃ (115e) 3-N═C(—SCH₃)NHCH₃ —H —CH₃ (116e) 3-CH₂P(═O)(OCH₃)₂ —H —CH₃

Among the present compounds (IV), the present compounds (IVf) represented by the compound numbers (1f) to (14f) are exemplified in Table 2f.

TABLE 2f The present compound (IVf): (IVf)

Com- pound No. X_(a) q_(a) t_(a) (1f) —OCH₂COOCH₃ —OCH₂CH═CH₂ —CH₃ (2f) —OCH₂COOCH₃ —OCH₂C≡CH —CH₃ (3f) —OCH₂COOCH₃ —OCH₂COOCH₃ —CH₃ (4f) —OCH₂COOH —OCH₂COOH —CH₃ (5f) —OCH₂CONH₂ —OCH₂CONH₂ —CH₃ (6f) —OCH₂COOCH₃ —OCH₂CN —CH₃ (7f) —OCH₂COOH —OCH₂CH₂OH —CH₃ (8f) —OCH₂COOCH₃ —OCH₂Ph —CH₃ (9f) —OCH₂COOH —OCH₂Ph —CH₃ (10f) —OCH₂COOCH₃ —OCH₂CH₂N(CH₃)₂ —CH₃ (11f) —OCH₂CH₂CH₂OH

—H (12f)

—CH₃ (13f) —OCH₂COOCH₃ —NHCH₂C≡CH —CH₃ (14f) —OCH₂CO —NHCH₂CH₂OCH₃ —CH₃ —NHCH₂CH₂OCH₃

Among the present compounds (IV), the present compounds (IVg) represented by the compound numbers (1g) to (11g) are exemplified in Table 2g.

TABLE 2g The present compound (IVg): (IVg)

Compound No. t_(a) (1g) —CH₂CH═CH₂ (2g) —CH₂C≡CH (3g) —CH₂COOCH₃ (4g) —CH₂COOH (5g) —CH₂CONH₂ (6g) —CH₂CN (7g) —CH₂COCH₃ (8g) —CH₂CH₂OCH₃ (9g) —CH₂Ph (10g) —Ph (11g)

The present compound has the ability to suppress transcription of a Type I collagen gene. The ability is important in improving tissue fibrosis because it decreases expression of a Type I collagen gene to induce a reduction in accumulation of collagen. Therefore, the present compound can be utilized as an active ingredient of a composition (medicament, cosmetic, food additive etc.) which can improve tissue fibrosis by decreasing expression of a Type I collagen gene to induce a reduction in accumulation of collagen.

A disease to which the transcription-suppressing composition of the present invention and the fibrosis-improving composition of the present invention can be applied includes, for example, a disease in which excessive accumulation of collagen causes fibrosis and then sclerosis of tissues, resulting in decreased function, cicatrization and the like in the tissues such as organs (i.e. fibrosing disease etc.). Specifically, the disease includes diseases and disorders such as hepatic cirrhosis, interstitial pulmonary disease, chronic renal failure (or disease resulting in chronic renal failure), hyperplasia scar after inflammation, postoperative scars or burn scars, scleroderma, arteriosclerosis, hypertension and the like. Incidentally, as an example of hepatic cirrhosis, it has been already known that C type or B type hepatitis virus induces chronic inflammation and then increased production of TGF-β, and thereby, hepatic fibrosis (particularly, accumulation of type I and type III collagen) is induced to cause hepatic cirrhosis (e.g. see Clin. Liver Dis., 7, 195-210(2003)). As an example of interstitial pulmonary disease, it has been thought that pneumonia caused by mites, viruses, tubercle bacilli or the like induces increased production of TGF-β and then pulmonary fibrosis, and thereby interstitial pulmonary disease is caused. For chronic renal failure such as diabetic nephropathy and IgA nephropathy, it has been already suggested that diabetic nephropathy is caused by increased level of TGF-β in renal glomeruli due to hyperglycemia and thereby induction of renal fibrosis (particularly accumulation of Type I and Type IV collagen), and IgA nephropathy is caused by induction of nephritis due to accumulation of IgA in renal glomeruli followed by increased level of TGF-β, and thereby induction of renal fibrosis (particularly accumulation of Type I and Type IV collagen) (e.g. see Am. J. Physiol. Renal Phsiol., 278, F830-F838(2000), Kidney Int. 64.149-159(2003)). A db/db mouse, a diabetic nephropathy model animal, develops hyperglycemia by overeating because it has a mutation in a leptin receptor for suppressing ingestion, and then spontaneously develops diabetes. In the db/db mouse, the blood glucose concentration is about 4 times higher than a normal mouse, and fibrosis of renal glomeruli and increased level of TGF-β are found (e.g. see Am. J. Pathol., 158, 1653-1663(2001)). An anti-Thy-1 rat, an IgA nephropathy model animal, is produced by administering an anti-Thy-1 antibody to a normal rat to artificially cause renal fibrosis. It has been shown that renal fibrosis is suppressed by administering an anti-TGF-β receptor antibody to the model animal (e.g. see Kidney Int., 60, 1745-1755(2001)). Although the cause of scleroderma is unknown, it has been found that skin fibrosis is improved by administering a TGF-β inhibitor to a Tsk mouse, which is a model animal therefor (e.g. see J. Invest. Dermatol., 118.461-470(2001)). Thus, a compound which suppresses the activity of TGF-β can be utilized as an active ingredient of a composition (medicament, cosmetic, food additive etc.) for inhibiting the collagen synthesis-promoting activity of TGF-β to suppress tissue fibrosis and thereby providing a fibrosing disease therapeutic effect.

Such transcription-suppressing composition and fibrosis-improving composition of the present invention comprise the present compound and an inert carrier. Such composition usually comprises 0.01% by weight to 99.99% by weight of the present compound and 99.99% by weight to 0.01% by weight of an inert carrier. The inert carrier is a pharmaceutically acceptable carrier or excipient. The transcription-suppressing composition and fibrosis-improving composition of the present invention may further comprise pharmaceutical additives, cosmetic additives, food additives and the like.

The present compound also inhibits the ability of TGF-β to promote transcription of a Type I collagen gene, as shown in Example 4 below. That is, the present compound is a TGF-β antagonist having the ability to suppress the activity of TGF-β. Therefore, the present compound can be also utilized as an active ingredient of a composition for suppressing the activity of TGF-β. It has been known that TGF-β has the ability to promote transition from a growth phase (hereinafter, also referred to as hair growth phase in some cases) to a regression phase (hereinafter, also referred to as a hair regression phase in some cases) in the hair life cycle [J. Invest. Dermatol., 111, 948-954(1998), FASEB J., 16, 1967-1969(2002)]. Further, it has been reported that an anti-TGF-β antibody, Fetuin, which is a TGF-β inhibitor, and the like antagonize the suppressing-activity of TGF-β on hair extension and exhibit a promoting-effect on hair extension [J. Invest. Dermaton., 118, 993-997(2002), JP-A 2000-342296]. Therefore, the present compound (and a TGF-β activity-suppressing composition containing the present compound as an active ingredient) may be utilized for inhibiting a promoting effect of TGF-β on transition to a hair regression phase to induce extension of a hair growth phase and thereby providing a hair-growing effect.

Such TGF-β suppressing composition and hair-growing composition of the present invention comprise the present compound and an inert carrier. Such composition usually comprises 0.01% by weight to 99.99% by weight of the present compound and 99.99% by weight to 0.01% by weight of an inert carrier. The inert carrier is a pharmaceutically acceptable carrier or excipient. The TGF-β suppressing composition and hair-growing composition of the present invention may further comprise pharmaceutical additives, cosmetic additives, food additives and the like.

A pharmaceutically acceptable carrier, excipient, pharmaceutical additive, food additive, cosmetic additive, a medicament additive, and the like contained in the above-described composition can be appropriately selected depending on the specific use thereof. In addition, the composition may be in a form of various solids, liquids and the like depending on the specific use thereof.

For example, when the present compound is used as an active ingredient of a medicament, specific examples of the medicament include oral preparations such as powders, fine granules, granules, tablets, syrups, capsules, suspensions, emulsions, extracts and pills; and parenteral preparations such as injections, transdermal absorbing agents such as external liquids and ointments, suppositories and local preparations.

Oral preparations can be prepared using carriers or excipients, and pharmaceutical additives such as binders, disintegrants, surfactants, lubricants, glidants, diluents, preservatives, coloring agents, flavors, stabilizers, humectants, antiseptics, antioxidants and the like, for example, gelatin, sodium alginate, starch, corn starch, white sugar, lactose, glucose, mannit, carboxymethylcellulose, dextrin, polyvinylpyrrolidone, crystalline cellulose, soybean lecithin, sucrose, fatty acid ester, talc, magnesium stearate, polyethylene glycol, magnesium silicate, anhydrous silicic acid and the like, according to a conventional method.

A dose of the oral preparation varies depending on the age, sex and weight of a mammal to be administered, the severity of disease, the kind and dosage form of the composition of the present invention, and the like. Usually, in the case of oral administration, about 1 mg to about 2 g per day, preferably about 5 mg to about 1 g per day of the active ingredient may be administered to an adult human. The daily dose may be also administered at one time or in several divided doses.

Among parenteral preparations, an injection can be prepared using such as a water-soluble solvent such as physiological saline or sterilized water Ringer solution, a water-insoluble solvent such as vegetable oil or fatty acid ester, an isotonic agent such as glucose or sodium chloride, pharmaceutical additives such as a solubilizer, a stabilizer, an antiseptic, a suspending agent and an emulsifying agent, and the like, according to a conventional method. A transdermal absorbing agent such as external liquid or a gel-like ointment, a suppository for rectal administration and the like can be also prepared according to a conventional method. For administering such parenteral preparations, they may be administered by injection (subcutaneously, intravenously etc.), transdermally, or rectally. A local preparation can be prepared, for example, by incorporating the present compound into a pellet of a sustained-release polymer such as ethylene vinyl acetate polymer. The pellet may be surgically transplanted into a tissue to be treated.

A dose of the parenteral preparation varies depending on the age, sex and weight of a mammal to be administered, the severity of disease, the kind and dosage form of the composition of the present invention, and the like. Usually, in the case of administration by injection, about 0.1 mg to about 500 mg of the active ingredient may be administered to an adult human. The daily dose may be also administered at one time or in several divided doses.

When the present compound is used by adding to cosmetics, specific examples of the form of a cosmetic with comprises the present compound include liquid, emulsion, cream, lotion, ointment, gel, aerosol, mousse and the like. Lotion can be prepared using cosmetic additives such as a suspending agent, an emulsifier, a preservative and the like, according to a conventional method.

A dose of the cosmetic varies depending on the age, sex and weight of a mammal to be administered, the severity of disease, the kind and dosage form of the composition of the present invention, and the like. Usually, about 0.01 mg to about 50 mg of the active ingredient may be administered to an adult human. The daily dose may be also administered at one time or in several divided doses.

When the present compound is used as a food additive, specific examples of the form of a food which comprises the additive include powder, a tablet, a beverage, an edible gel or a mixed liquid of the gel and syrup, for example, general beverage and food and luxury food and beverage such as seasonings, Japanese confectioneries, western confectioneries, ice confectionaries, beverage, spreads, pastes, pickles, bottled or canned products, processed domestic animal meats, processed fish meats or marine product, processed dairy or egg products, processed vegetables, processed fruits, processed cereals and the like. Alternatively, the present compound can be also added to feeds or provenders for rearing animals such as livestocks, poultry, honey bee, silkworm, fish and the like.

A dose of the food varies depending on the age, sex and weight of a mammal to be administered, the severity of disease, the kind and dosage form of the composition of the present invention, and the like. Usually, about 0.1 mg to about 500 mg of the active ingredient may be administered to an adult human. The daily dose may be also administered at one time or in several divided doses.

EXAMPLE

The following Examples further illustrate the present invention.

Example 1

Synthesis of the present benzaldehyde derivative and the present pyridinecarbaldehyde derivative will be described in Examples 1-1 to 1-24.

Example 1-1 Synthesis of the Present Benzaldehyde Derivative Compound No. (a)

To a mixture of 12.31 g of 3-aminobenzyl alcohol, 160 ml of tetrahydrofuran and 12.41 g of triethylamine was added a solution of 11.42 g of methoxyacetyl chloride in 40 ml of tetrahydrofuran at 10° C. After stirred at room temperature for 1.5 hours, insolubles were filtered and the filtrate was concentrated under reduced pressured. The resulting residue was dissolved in 200 ml of ethyl acetate. The organic layer was washed successively with water, diluted hydrochloric acid and an aqueous saturated sodium chloride solution, dried over anhydrous magnesium sulfate, and then concentrated. The residue was subjected to silica gel column chromatography to obtain 15.88 g of oily (3-methoxyacetylamino)benzyl alcohol.

¹H-NMR (400MHz, CDCl₃) δ (ppm): 1.83 (t, 1H, J=5.1 Hz), 3.50 (s, 3H), 4.01 (s, 2H), 4.69 (d, 2H, J=4.4 Hz), 7.13 (dd, 1H, J=0.5, 7.1 Hz), 7.33 (t, 1H, J=7.8 Hz), 7.50 (dd, 1H, J=1.0, 8.1 Hz), 7.59 (s, 1H), 8.26 (broad s, 1H).

To a mixture of 11.40 g of oxalyl chloride and 200 ml of dichloromethane was added dropwise a solution of 14 ml of dimethyl sulfoxide in 30 ml of dichloromethane at −60° C. for 15 minutes. After stirred at −60° C. for 10 minutes, a solution of 15.88 g of 3-(methoxyacetylamino)benzyl alcohol in 70 ml of dichloromethane was added dropwise at −60° C. for 20 minutes. After stirred at −60° C. for 10 minutes, 24.82 g of triethylamine was added dropwise at −60° C. for 20 minutes. After stirred at room temperature for 45 minutes, 500 ml of water was added to the reaction solution, followed by extraction with 300 ml of ethyl acetate. The organic layer was washed with an aqueous saturated sodium chloride solution, dried over anhydrous magnesium sulfate, and then concentrated to obtain 14.93 g of 3-(methoxyacetylamino)benzaldehyde [Compound No. (a)] as a white crystal.

¹H-NMR (400 MHz, CDCl₃) δ (ppm): 3.53 (s, 3H), 4.05 (s, 2H), 7.52 (t, 1H, J=7.8 Hz), 7.65 (d, 1H, J=7.6 Hz), 7.93 (d, 1H, J=8.0 Hz), 8.06 (s, 1H), 8.41 (broad s, 1H), 10.01 (s, 1H)

Example 1-2 Synthesis of the Present Benzaldehyde Derivative Compound No. (b)

To a mixture of 200 ml of tetrahydrofuran, 26.00 g of pyridine and 20.70 g of glycine methyl ester hydrochloride was added a solution of 16.00 g of 3-formylbenzoic acid chloride in 20 ml of tetrahydrofuran at 10° C. After stirred at room temperature for 60 hours, insolubles were filtered and the filtrate was concentrated under reduced pressure. The resulting residue was subjected to silica gel column chromatography to obtain 4.23 g of oily 3-[[(methyoxycarbonylmethyl)amino]carbonyl]benzaldehyde [Compound No. (b)].

¹H-NMR (400 MHz, CDCl₃) δ (ppm): 3.83 (s, 3H), 4.29 (d, 2H, J=4.9 Hz), 6.78 (broad s, 1H), 7.65 (t, 1H, J=7.6 Hz), 8.04 (d, 1H, J=7.6 Hz), 8.11 (d, 1H, J=7.6 Hz), 8.31 (s, 1H), 10.08 (s, 1H)

Example 1-3 Synthesis of the Present Benzaldehyde Derivative Compound No. (c)

According to the same manner as that of Example 1-2 except that 15.40 g of 4-formylbenzoic acid chloride was used in place of 3-formylbenzoic acid chloride, 5.79 g of 4-[[(methoxycarbonylmethyl)amino]carbonyl]benzaldehyde [Compound No. (c)] was obtained as a pale yellow solid.

¹H-NMR (400 MHz, CDCl₃) δ (ppm): 3.83 (s, 3H), 4.29 (s, 2H), 6.73 (broad s, 1H), 7.97 (s, 4H), 10.09 (s, 1H)

Example 1-4 Synthesis of the Present Benzaldehyde Derivative Compound No. (d)

To a mixture of 200 ml of tetrahydrofuran, 16.70 g of triethylamine and 12.40 g of 2-methoxyethylamine was added a solution of 16.00 g of 3-formylbenzoic acid chloride in 20 ml of tetrahydrofuran at room temperature. After stirred at room temperature for 6 hours, insolubles were filtered and the filtrate was concentrated under reduced pressure. The resulting residue was subjected to silica gel column chromatography to obtain 10.79 g of oily 3-[(2-methoxyethyl)aminocarbonyl]benzaldehyde [Compound No. (d)].

¹H-NMR (400 MHz, CDCl₃) δ (ppm): 3.41 (s, 3H), 3.59 (t, 2H, J=4.6 Hz), 3.69 (dt, 2H, J=5.3, 5.4 Hz), 7.64 (t, 1H, J=7.6 Hz), 8.03 (dt, 1H, J=1.2, 7.6 Hz), 8.10 (dt, 1H, J=1.2, 7.8 Hz), 8.27 (s, 1H), 10.08 (s, 1H)

Example 1-5 Synthesis of the Present Benzaldehyde Derivative Compound No. (e)

To a mixture of 3.73 g of sodium hydride (60% oily) and 150 ml of dimethylformamide was added dropwise a solution of 16.53 g of diethyl cyanomethylphosphonate in 12 ml of dimethylformamide under ice-cooling. After stirred at room temperature for 1 hour, a solution of 14.85 g of 3-([1,3]dioxolan-2-yl)benzaldehyde in 40 ml of dimethylformamide was added. After stirred at 50° C. for 30 minutes, ice water was added to the mixture, followed by extraction with ethyl acetate. The organic layer was washed with an aqueous saturated sodium chloride solution, dried over anhydrous sodium sulfate, and then concentrated under reduced pressure. The resulting residue was subjected to silica gel column chromatography to obtain 11.91 g of a cis-trans isomer mixture of oily 2-[3-(2-cyanoethenyl)phenyl]-[1,3]dioxolane.

11.91 g of the cis-trans isomer mixture of 2-[3-(2-cyanoethenyl)phenyl]-[1,3]dioxolane was dissolved in 180 ml of tetrahydrofuran, and thereto 40 ml of 6 N hydrochloric acid was added dropwise under ice-cooling. After stirred at room temperature overnight, the reaction solution was concentrated under reduced pressure, and extracted with t-butyl methyl ether and then ethyl acetate. The organic layers were combined, and washed successively with an aqueous saturated sodium bicarbonate solution and then an aqueous saturated sodium chloride solution. After dried over anhydrous magnesium sulfate, crystals obtained by concentration under reduced pressured were filtered to obtain 4.90 g of trans-3-(2-cyanoethenyl)benzaldehyde [Compound No. (e)] as a white solid.

¹H-NMR (400 MHz, CDCl₃) δ (ppm): 5.96 (d, 1H, J=16.8 Hz), 7.47 (d, 1H, J=16.8 Hz), 7.59-7.63 (m, 1H), 7.71 (d, 1H, J=7.6 Hz), 7.93-7.97 (m, 2H), 10.05 (s, 1H)

Example 1-6 Synthesis of the Present Benzaldehyde Derivative Compound No. (f)

To a mixture of 1.00 g of 3-hydroxybenzaldehyde, 25 ml of tetrahydrofuran, 2.40 g of triphenylphosphine and 0.78 ml of 2-methylthioethanol was added dropwise 3.50 ml of diethyl azodicarboxylate (40% toluene solution), and the mixture was stirred at room temperature for 15.5 hours. The reaction solution was concentrated under reduced pressure, and the resulting residue was subjected to silica gel column chromatography to obtain 0.71 g of oily 3-(2-methylthioethoxy)benzaldehyde [Compound No. (f)].

¹H-NMR (300 MHz, CDCl₃) δ (ppm): 2.23 (s, 3H), 2.91 (t, 2H, J=6.0 Hz), 4.22 (t, 2H, J=6.0 Hz), 7.17-7.21 (m, 1H), 7.39-7.47 (m, 3H), 9.98 (s, 1H).

Example 1-7 Synthesis of the Present Benzaldehyde Derivative Compound No. (g)

A mixture of 1.99 g of 3-(bromomethyl)benzaldehyde, 0.80 g of sodium hydroxide and 8 ml of ethylene glycol was heated at 55° C. for 6 hours. After water was added, the mixture was extracted with chloroform and then washed with an aqueous saturated sodium chloride solution. After dried over anhydrous sodium sulfate and then concentration under reduced pressure, the resulting residue was subjected to silica gel column chromatography to obtain 0.79 g of oily 3-[(2-hydroxyethoxy)methyl]benzaldehyde [Compound No. (g)].

¹H-NMR (270 MHz, CDCl₃) δ (ppm): 2.00 (broad s, 1H), 3.59-3.80 (m, 4H), 4.65 (s, 2H), 7.51-7.56 (m, 1H), 7.63 (d, 1H, J=7.4 Hz), 7.82 (d, 1H, J=7.4 Hz), 7.87 (s, 1H), 10.03 (s, 1H).

Example 1-8 Synthesis of the Present Benzaldehyde Derivative Compound No. (h)

To a solution of 15.0 g of 3-aminobenzyl alcohol in 120 ml of tetrahydrofuran was added dropwise a solution of 18 ml of 2-methoxyethyl chloroformate in 70 ml of tetrahydrofuran under ice-cooling. After stirred for 30 minutes under ice-cooling and then at room temperature for 30 minutes, additional 2 ml of 2-methoxyethyl chloroformate was added, and the mixture was stirred at room temperature for 1 hour. After ethyl acetate was added, the mixture was washed successively with an aqueous saturated sodium bicarbonate solution and then an aqueous saturated sodium chloride solution, dried over anhydrous magnesium sulfate, and then concentrated to obtain 30.2 g of 3-[(2-methoxyethoxy)carbonylamino]benzyl alcohol.

¹H-NMR (400 MHz, CDCl₃) δ (ppm): 1.82 (t, 1H, J=5.2 Hz), 3.41 (s, 3H), 3.63-3.65 (m, 2H), 4.31-4.34 (m, 2H), 4.67 (d, 2H, J=5.2 Hz), 6.77 (broad s, 1H), 7.05-7.08 (m, 1H), 7.27-7.31 (m, 2H), 7.40 (s, 1H).

To a mixture of 13 ml of oxalyl chloride and 400 ml of dichloromethane was added dropwise a solution of 23 ml of dimethyl sulfoxide in 40 ml of dichloromethane at −60° C. for 15 minutes. After stirred at −60° C. for 10 minutes, a solution of 30.2 g of 3-[(2-methoxyethoxy)carbonylamino]benzyl alcohol in 100 ml of dichloromethane was added dropwise at −60° C. for 25 minutes. After stirred at −60° C. for 20 minutes, 56 ml of triethylamine was added dropwise at −60° C. for 15 minutes. After stirred at room temperature for 45 minutes, water was added to the reaction solution, followed by extraction with ethyl acetate. The organic layer was washed successively with water and then an aqueous saturated sodium chloride solution. After dried over anhydrous magnesium sulfate and then concentration, the resulting crude crystals were washed with t-butyl methyl ether and then dried to obtain 17.55 g of 3-[(2-methoxyethoxy)carbonylamino]benzaldehyde [Compound No. (h)] as a white solid.

¹H-NMR (400 MHz, CDCl₃) δ (ppm): 3.43 (s, 3H), 3.65-3.67 (m, 2H), 4.35-4.37 (m, 2H), 6.84 (broad s, 1H), 7.48 (t, 1H, J=6.8 Hz), 7.59 (d, 1H, J=6.8 Hz), 7.67 (d, 1H, J=6.8 Hz), 7.90 (s, 1H), 9.99 (s, 1H).

Example 1-9 Synthesis of the Present Benzaldehyde Derivative Compound No. (i)

To a solution of 1.23 g of 3-aminobenzyl alcohol in 12 ml of tetrahydrofuran was added dropwise a solution of 1.32 ml of phenyl chloroformate in 5 ml of tetrahydrofuran under ice-cooling. After stirred at room temperature for 30 minutes, a solvent was distilled off under reduced pressure, and the resulting residue was dissolved in 10 ml of dimethyl sulfoxide. 2.2 ml of 2-methoxyethylamine was added, and the mixture was stirred at 70° C. for 40 minutes. This was cooled to room temperature, ethyl acetate and water were added, and the layers were separated. Water was distilled off from the aqueous layer under reduced pressure, and sodium chloride was added, followed by extraction with ethyl acetate. After dried over anhydrous magnesium sulfate and then concentration, the resulting residue was subjected to silica gel column chromatography to obtain 0.67 g of oily 3-[(2-methoxyethyl)aminocarbonylamino]benzyl alcohol.

¹H-NMR (270 MHz, CDCl₃) δ (ppm): 3.33 (s, 3H), 3.36 (t, 2H, J=5.4 Hz), 3.45 (t, 2H, J=5.4 Hz), 4.53 (d, 2H, J=5.4 Hz), 5.88 (t, 1H, J=5.4 Hz), 6.93 (d, 1H, J=5.4 Hz), 7.16 (d, 1H, J=7.6 Hz), 7.21 (s, 1H), 7.27 (d, 1H, J=5.4 Hz), 7.64 (s, 1H), 8.00 (s, 1H).

To a mixture of 2.64 g of oxalyl chloride and 50 ml of dichloromethane was added dropwise a solution of 3.24 g of dimethyl sulfoxide in 30 ml of dichloromethane at −60° C. for 10 minutes. After stirred at −60° C. for 20 minutes, a solution of 3.72 g of 3-[(2-methoxyethyl)aminocarbonylamino]benzyl alcohol in 30 ml of dichloromethane was added dropwise at −60° C. for 1 hour. After stirred at −60° C. for 15 minutes, 9.24 g of triethylamine was added dropwise at −60° C. for 25 minutes. After stirred at room temperature for 1 hour, water was added to the reaction solution, and the layers were separated. The organic layer was washed with an aqueous saturated sodium chloride solution, dried over anhydrous sodium sulfate, and then concentrated to obtain 2.79 g of 3-[(2-methoxyethyl)aminocarbonylamino]benzaldehyde [Compound No. (i)] as a white crystal.

¹H-NMR (270 MHz, CDCl₃) δ (ppm): 3.38 (s, 3H), 3.43-3.48 (m, 2H), 3.53 (t, 2H, J=4.3 Hz), 5.75 (broad s, 1H), 7.40 (t, 1H, J=7.8 Hz), 7.50 (d, 1H, J=7.6 Hz), 7.71 (d, 1H, J=7.8 Hz), 7.80 (s, 1H), 7.81 (s, 1H), 9.92 (s, 1H)

Example 1-10 Synthesis of the Present Benzaldehyde Derivative Compound No. (j)

A mixture of 10.18 g of 3-formylbenzoic acid, 6.99 g of methanesulfonamide, 200 ml of dichloromethane, 8.95 g of dimethylaminopyridine, 15.22 g of dicyclohexylcarbodiimide and 100 ml of tetrahydrofuran was stirred at room temperature. The reaction solution was concentrated under reduced pressure, dissolved in ethyl acetate, a 1 N aqueous sodium hydroxide solution was added, and the layers were separated. To the aqueous layer was added 2 N hydrochloric acid to adjust to pH 1, this was extracted with ethyl acetate, dried over anhydrous sodium sulfate, and then concentrated to obtain 4.01 g of 3-[(methanesulfonyl)aminocarbonyl]benzaldehyde [Compound No. (j)] as a white solid.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 3.38 (s, 3H), 7.75 (t, 1H, J=7.6 Hz), 8.14-8.23 (m, 2H), 8.46 (s, 1H), 10.08 (s, 1H), 12.39 (broad s, 1H).

Example 1-11 Synthesis of the Present Benzaldehyde Derivative Compound No. (k)

To a mixture of 1.93 g of cyanoacetamide sulfate and 5 ml of water was added dropwise a solution of 3.34 g of 3-formylbenzoic acid chloride in 7 ml of toluene under ice-cooling. 2.93 g of sodium carbonate was added, and this was stirred at room temperature for 2 hours. The resulting crystals were filtered and washed successively with water, toluene and t-butyl methyl ether to obtain 1.80 g of 3-[(cyanomethyl)aminocarbonyl]benzaldehyde [Compound No. (k)].

¹H-NMR (400 MHz, CDCl₃+DMSO-d₆ (1 drop)) δ (ppm): 4.34 (d, 2H, J=5.4 Hz), 7.64-7.67 (m, 1H), 8.03-8.05 (m, 1H), 8.23-8.26 (m, 1H), 8.46-8.47 (m, 1H), 9.11 (broad s, 1H), 10.09 (s, 1H).

Example 1-12 Synthesis of the Present Benzaldehyde Derivative Compound No. (1)

To a mixture of 0.67 g of magnesium and 10 ml of tetrahydrofuran was added a catalytic amount of iodine, and added dropwise a solution of 6.0 g of 1-bromo-3-(2,2-difluoroethenyl)benzene in 20 ml of tetrahydrofuran at 55° C. After stirred at room temperature for 15 minutes, a solution of 3.98 g of 1-formylpiperidine in 5 ml of tetrahydrofuran was added dropwise. This was heated under reflux for 15 minutes, and ice water and 10% hydrochloric acid were added, followed by extraction with t-butyl methyl ether. The organic layer was washed with an aqueous saturated sodium chloride solution, dried over anhydrous magnesium sulfate and then concentrated. The resulting residue was subjected to silica gel column chromatography to obtained 1.13 g of oily 3-(2,2-difluoroethenyl)benzaldehyde [Compound No. (1)].

¹H-NMR (400 MHz, CDCl₃) δ (ppm): 5.36 (dd, 1H, J=3.4, 25.9 Hz), 7.52 (t, 1H, J=7.6 Hz), 7.59 (d, 1H, J=7.8 Hz), 7.75 (d, 1H, J=7.6 Hz), 7.83 (s, 1H), 10.01 (s, 1H).

Example 1-13 Synthesis of the Present Benzaldehyde Derivative Compound No. (m)

To a solution of 4.48 g of a cis-trans isomer mixture of 2-[3-(2-cyanoethenyl)phenyl]-[1,3]dioxolane in 100 ml of ethyl acetate was added 0.60 g of 5% palladium carbon to perform hydrogenation. The catalyst was filtered by filtration with Celite, and the filtrate was concentrated under reduced pressure to obtain 3.52 g of 2-[3-(2-cyanoethyl)phenyl]-[1,3]dioxolane.

¹H-NMR (400 MHz, CDCl₃) δ (ppm): 2.62 (t, 2H, J=7.6 Hz), 2.98 (t, 2H, J=7.6 Hz), 4.04-4.13 (m, 4H), 5.80 (s, 1H), 7.24 (d, 1H, J=7.1 Hz), 7.34-7.38 (m, 3H).

To 3.52 g of 2-[3-(2-cyanoethyl)phenyl]-[1,3]dioxolane was added 60 ml of tetrahydrofuran to dissolve this, and 20 ml of 6 N hydrochloric acid was added. After stirred at room temperature overnight and then concentrated under reduced pressure, ethyl acetate was added, and this was washed successively with an aqueous potassium carbonate solution and an aqueous saturated sodium chloride solution. After dried over anhydrous magnesium sulfate, concentration under reduced pressure afforded 2.68 g of 3-(2-cyanoethyl)benzaldehyde [Compound No. (m)].

¹H-NMR (400 MHz, CDCl₃) δ (ppm): 2.69 (t, 2H, J=7.3 Hz), 3.06 (t, 2H, J=7.3 Hz), 7.53-7.56 (m, 2H), 7.76-7.82 (m, 2H), 10.02 (s, 1H).

Examples 1-14 Synthesis of the Present Benzaldehyde Derivative Compound No. (n)

To a mixture of 12.21 g of 3-hydroxybenzaldehyde, 14.00 g of 2-chloroacetamide and 60 ml of dimethylformamide was added 20.70 g of potassium carbonate, and this was heated to stir at 90° C. for 2 hours. After cooled to room temperature, insolubles were filtered and the filtrate was concentrated under reduced pressure. The resulting residue was dissolved in tetrahydrofuran by heating. Insolubles were filtered and the filtrate was concentration under reduced pressure. The resulting crude crystals were washed with a mixed solution of tetrahydrofuran and t-butyl methyl ether and dried to obtain 13.05 g of 3-(aminocarbonylmethoxy)benzaldehyde [Compound No. (n)] as a crystal.

¹H-NMR (300 MHz, DMSO-d₆) δ (ppm): 4.53 (s, 2H), 7.29-7.60 (m, 6H), 9.98 (s, 1H).

Example 1-15 Synthesis of the Present Benzaldehyde Derivative Compound No. (o)

To a mixture of 3.05 g of 3-hydroxybenzaldehyde, 2.3 ml of bromoacetone and 30 ml of dimethylformamide was added 4.15 g of potassium carbonate, and this was heated and stirred at 70° C. for 30 minutes. After cooled to room temperature, insolubles were filtered and the filtrate was concentrated under reduced pressure. Water was added to the resulting residue, and then extracted with ethyl acetate. The organic layer was washed successively with water and an aqueous saturated sodium chloride solution, dried over anhydrous magnesium sulfate, and then concentrated. The resulting residue was subjected to silica gel column chromatography to obtain 0.76 g of oily 3-(2-oxo-propoxy)benzaldehyde [Compound No. (o)].

1H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.18 (s, 3H), 4.94 (s, 2H), 7.23-7.30 (m, 1H), 7.37-7.38 (m, 1H), 7.49-7.53 (m, 2H), 9.97 (s, 1H).

Example 1-16 Synthesis of the Present Benzaldehyde Derivative Compound No. (p)

A mixture of 30 ml of tetrahydrofuran, 12 ml of triethylamine and 4.11 g of aspartic acid dimethyl ester hydrochloride was added dropwise to a solution of 3.50 g of 3-formylbenzoic acid chloride in 30 ml of tetrahydrofuran at 10° C. After stirred at room temperature for 6 hours, insolubles were filtered and the filtrate was concentrated under reduced pressure. The resulting residue was subjected to silica gel column chromatography to obtain 3.01 g of oily 2-[3-formyl-(benzoylamino)]succinic acid dimethyl ester [Compound No. (p)].

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.82-3.03 (m, 2H), 3.39 (s, 3H), 3.44 (s, 3H), 4.84-4.92 (m, 1H), 7.68-7.95 (m, 1H), 8.12-8.18 (m, 2H), 8.39 (s, 1H), 9.18 (d, 1H, J=8.1 Hz), 10.09 (s, 1H)

Example 1-17 Synthesis of the Present Pyridinecarbaldehyde Derivative [Compound No. (q)]

A mixture of 5.15 g of 2-carboxy-6-formylpyridine and 50 ml of thionyl chloride was stirred under reflux for 1 hour and then concentrated under reduced pressure. The resulting acid chloride was dissolved in 30 ml of tetrahydrofuran, and the solution was added dropwise to a mixture of 30 ml of tetrahydrofuran, 3.12 g of triethylamine and 2.31 g of 2-methoxyethylamine under ice-cooling. After allowed to stand at room temperature overnight, this was concentrated under reduced pressure, and the resulting residue was subjected to silica gel column chromatography to obtain 3.28 g of 6-formyl-2-[(2-methoxyethyl)aminocarbonyl]pyridine [Compound No. (q)] as a white solid.

¹H-NMR (270 MHz, CDCl₃) δ (ppm): 3.43 (s, 3H), 3.56-3.65 (m, 2H), 3.70-3.76 (m, 2H), 8.02-8.12 (m, 2H), 8.34 (broad s, 1H), 8.43-8.46 (m, 1H), 10.11 (s, 1H).

Example 1-18 Synthesis of the Present Benzaldehyde Derivative Compound No. (r)

To a solution of 4.0 g of 3-[(2-methoxyethyl)aminosulfonyl]benzoic acid in 200 ml of tetrahydrofuran was added dropwise a solution of 1.07 M borane-tetrahydrofuran complex in 43.5 ml of tetrahydrofuran under ice-cooling, and this was stirred for 30 minutes, and stirred at room temperature overnight. After 40 ml of methanol was added dropwise under ice-cooling, 100 ml of 2 N hydrochloric acid was added dropwise. After warmed to room temperature, the solvent was distilled off under reduced pressure, followed by extraction with ethyl acetate. The organic layer was dried over anhydrous magnesium sulfate, and concentrated under reduced pressure to obtain 3.0 g of oily 3-[(2-methoxyethyl)aminosulfonyl]benzyl alcohol.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.86-2.92 (m, 2H), 3.16 (s, 3H), 3.27-3.33 (m, 2H), 4.58 (d, 2H, J=5.6 Hz), 5.42 (t, 1H, J=5.6 Hz), 7.50-7.78 (m, 5H).

To a mixture of 1.71 g of oxalyl chloride and 30 ml of dichloromethane was added dropwise a solution of 2.3 g of dimethyl sulfoxide in 4 ml of dichloromethane at −60° C. for 35 minutes. After stirred at −60° C. for 20 minutes, a solution of 3.0 g of 3-[(2-methoxyethyl)aminosulfonyl]benzyl alcohol in 22 ml of dichloromethane was added dropwise at −60° C. for 1.5 hours. After stirred at −60° C. for 1 hour, 5.1 ml of triethylamine was added dropwise at −60° C. for 25 minutes. After stirred at room temperature for 3 hours, water was added to the reaction solution, and the layers were separated. The organic layer was washed with water, dried over anhydrous sodium sulfate, and then concentrated. The resulting residue was subjected to silica gel column chromatography to obtain 2.07 g of oily 3-[(2-methoxyethyl)aminosulfonyl]benzaldehyde [Compound No. (r)].

¹H-NMR (300 MHz, CDCl₃) δ (ppm): 3.15-3.20 (m, 2H), 3.28 (s, 3H), 3.41-3.44 (m, 2H), 5.00 (t, 1H, J=6.0 Hz), 7.72 (t, 1H, J=7.5 Hz), 8.09-8.15 (m, 2H), 8.37 (s, 1H), 10.09 (s, 1H).

Example 1-19 Synthesis of the Present Benzaldehyde Derivative Compound No. (s)

To a solution of 5.63 g of 3-([1,3]dioxolan-2-yl)benzoic acid in 60 ml of tetrahydrofuran were added 3.3 ml of ethyl chloroformate and 4.8 ml of triethylamine under ice-cooling. After stirred for 10 minutes under ice-cooling, insolubles were filtered. This solution was added dropwise to a mixture of 3.63 g of methoxyamine hydrochloride, 20 ml of tetrahydrofuran, 6 ml of triethylamine and 20 ml of dimethylformamide. After stirred at room temperature for 8 hours, insolubles were filtered, and the filtrate was concentrated under reduced pressure. The resulting residue was dissolved in 30 ml of tetrahydrofuran, and 15 ml of 2 N hydrochloric acid was added dropwise, followed by stirring at room temperature for 8 hours. 20 ml of a 2 N aqueous sodium hydroxide solution was added dropwise under ice-cooling, and this was extracted with ethyl acetate. The organic layer was washed with an aqueous saturated sodium chloride solution, dried over anhydrous sodium sulfate, and concentrated. The residue was subjected to silica gel column chromatography to obtain 1.50 g of 3-(methoxyaminocarbonyl)benzaldehyde [Compound No. (s)] as a white solid.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 3.73 (s, 3H), 7.72 (t, 1H, J=7.7 Hz), 8.05-8.10 (m, 2H), 8.28 (s, 1H), 10.07 (s, 1H), 11.98 (broad s, 1H).

Example 1-20 Synthesis of the Present Benzaldehyde Derivative Compound No. (t)

According to the same manner as that of Example 1-19 except that 4.93 g of allyloxyamine hydrochloride was used in place of methoxyamine hydrochloride, 1.55 g of 3-(allyloxyaminocarbonyl)benzaldehyde [Compound No. (t)] was obtained as a white solid.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 4.44 (d, 2H, J=5.9 Hz), 5.26-5.40 (m, 2H), 5.94-6.09 (m, 1H), 7.72 (t, 1H, J=7.7 Hz), 8.04-8.10 (m, 2H), 8.27 (s, 1H), 10.07 (s, 1H), 11.90 (broad s, 1H).

Example 1-21 Synthesis of the Present Benzaldehyde Derivative Compound No. (u)

To a mixture of 1.00 g of 3-(bromomethyl)benzaldehyde and 20 ml of ethanol were added 0.65 ml of methyl thioglycolate and 0.47 g of potassium carbonate, and the mixture was stirred at room temperature for 2.5 hours. To the reaction mixture was added diethyl ether, this was washed with an aqueous saturated sodium chloride solution, dried over anhydrous sodium sulfate, and then concentrated under reduced pressure. The resulting residue was subjected to silica gel column chromatography to obtain 0.36 g of oily 3-[(methoxycarbonylmethylthio)methyl]benzaldehyde [Compound No. (u)].

¹H-NMR (270 MHz, CDCl₃) δ (ppm): 3.08 (s, 2H), 3.73 (s, 3H), 3.91 (s, 2H), 7.51 (dd, 1H, J=7.6 Hz), 7.64 (d, 1H, J=7.6 Hz), 7.78-7.81 (m, 1H), 7.86 (s, 1H), 10.02 (s, 1H).

Example 1-22 Synthesis of the Present Benzaldehyde Derivative Compound No. (v)

To a suspension of 4.58 g of 3-(cyanobenzyl)triphenylphosphonium bromide in 15 ml of tetrahydrofuran was added 0.73 g of sodium hydride (60% oily) under ice-cooling, and this was stirred at room temperature for 1 hour. 1.01 g of tetrahydro-4H-pyran-4-one was added thereto, this was stirred at room temperature for 1 hour, 2 ml of dimethylformamide was added, and this was further stirred at room temperature for 5 hours. Water was added to the reaction solution, and this was extracted with ethyl acetate, and washed with an aqueous saturated sodium chloride solution. After dried over anhydrous magnesium sulfate, the solvent was distilled off under reduced pressure, and the resulting residue was subjected to silica gel column chromatography to obtain 0.20 g of yellow oily 3-[(tetrahydropyran-4-ylidene)methyl]benzonitrile.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.35 (t, 2H, J=5.4 Hz), 2.43 (t, 2H, J=5.4 Hz), 3.58 (t, 2H, J=5.4 Hz), 3.68 (t, 2H, J=5.4 Hz), 6.36 (s, 1H), 7.51-7.56 (m, 2H), 7.66-7.70 (m, 2H).

To a solution of 0.20 g of 3-[(tetrahydropyran-4-ylidene)methyl]benzonitrile in 7 ml of toluene was added dropwise a 1.5 M solution of diisobutylaluminum hydride in 1.24 ml of toluene at room temperature. After stirred at room temperature for 7 hours, an aqueous ammonium chloride solution was added to the reaction solution, and this was extracted with ethyl acetate, and washed with an aqueous saturated sodium chloride solution. After dried over anhydrous magnesium sulfate, the solvent was distilled off under reduced pressure, and the resulting residue was subjected to silica gel column chromatography to obtain 0.06 g of yellow oily 3-[(tetrahydropyran-4-ylidene)methyl]benzaldehyde [Compound No. (v)].

¹H-NMR (270 MHz, CDCl₃) δ (ppm): 2.43 (t, 2H, J=5.4 Hz), 2.52 (t, 2H, J=5.4 Hz), 3.68 (t, 2H, J=5.4 Hz), 3.80 (t, 2H, J=5.4 Hz), 6.37 (s, 1H), 7.44-7.53 (m, 2H), 7.71-7.75 (m, 2H), 10.01 (s, 1H)

Example 1-23 Synthesis of the Preset Benzaldehyde Derivative Compound No. (w)

To a solution of 4.93 g of m-aminobenzyl alcohol in 50 ml of tetrahydrofuran was added dropwise a solution of 3.7 ml of chloroglyoxylic acid methyl ester in 20 ml of tetrahydrofuran, and this was stirred at room temperature for 1.5 hours. To the reaction solution was added water, and this was extracted with ethyl acetate, and washed with an aqueous saturated sodium chloride solution. After dried over anhydrous magnesium sulfate, the solvent was distilled off under reduced pressure, and the resulting residue was subjected to silica gel column chromatography to obtain 5.10 g of 3-[(methoxycarbonyl)carbonylamino]benzyl alcohol as a white solid.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 3.85 (s, 3H), 4.47 (d, 2H, J=5.7 Hz), 5.23 (t, 1H, J=5.7 Hz), 7.09 (d, 1H, J=7.6 Hz), 7.30 (t, 1H, J=7.8 Hz), 7.58 (d, 1H, J=8.1 Hz), 7.73 (s, 1H), 10.76 (s, 1H)

To a solution of 1.69 g of 3-[(methoxycarbonyl)carbonylamino]benzyl alcohol in 20 ml of acetone was added 3.47 g of manganese dioxide, this was stirred at room temperature for 2 hours, 3.92 g of manganese dioxide was further added, and this was stirred at room temperature for 18 hours. The reaction solution was filtered with Celite, the filtrate was concentrated under reduced pressure, and the resulting residue was subjected to silica gel column chromatography to obtain 0.53 g of 3-[(methoxycarbonyl)carbonylamino]benzaldehyde [Compound No. (w)] as a white solid.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 3.87 (s, 3H), 7.61 (t, 1H, J=7.6 Hz), 7.72 (d, 1H, J=7.8 Hz), 8.00 (d, 1H, J=8.1 Hz), 8.34 (s, 1H), 9.99 (s, 1H), 11.08 (s, 1H).

Example 1-24 Synthesis of the Present Benzaldehyde Derivative Compound No. (x)

A mixture of 0.60 g of 3-(bromomethyl)benzaldehyde and 0.45 ml of trimethyl phosphite was stirred at 100° C. for 3 hours. The reaction mixture was subjected to silica gel column chromatography to obtain 0.62 g of oily dimethyl (3-formylbenzyl)phosphonate [Compound No. (x)].

¹H-NMR (270 MHz, CDCl₃) δ (ppm): 3.24 (d, 2H, J=21.9 Hz), 3.70 (d, 6H, J=11.1 Hz), 7.48-7.61 (m, 2H), 7.78-7.81 (m, 2H), 10.02 (s, 1H).

Example 2 Synthesis of the Present Compound will be Described in Examples a-1 to a-88, b-1 to b-11, c-1 to c-11, d-1, e-1 to e-19 and f-1 Example a-1 Synthesis of the Present Compound [Compound No. (7a)] by Process A

In a mixture of 9 ml of pyridine and 150 μl of piperidine were dissolved 0.75 g of 3-acetyl-4-hydroxy-6-methyl-2(1H)-pyridinone and 0.75 g of 3-[(methoxycarbonyl)methoxy]benzaldehyde, and the solution was heated under reflux for 4 hours. After cooled to room temperature, 20 ml of water was added, precipitated crystals were filtered, washed with water, washed with tetrahydrofuran, and dried to obtain 0.42 g of 4-hydroxy-3-[3-[3-[(methoxycarbonyl)methoxy]phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone [Compound No. (7a)] as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.21 (s, 3H), 3.72 (s, 3H), 4.87 (s, 2H), 5.88 (s, 1H), 7.00-7.06 (m, 1H), 7.24 (s, 1H), 7.30-7.45 (m, 2H), 7.77 (d, 1H, J=16.2 Hz), 8.50 (d, 1H, J=15.7 Hz), 11.58 (s, 1H), 13.61 (s, 1H).

Example a-2 Synthesis of the Present Compound [Compound No. (8a)] by Process A

According to the same manner as that of Example a-1 except that 0.73 g of 4-formyl-2-methoxyphenoxyacetic acid methyl ester was used in place of 3-[(methoxycarbonyl)methoxy]benzaldehyde, 0.93 g of 4-hydroxy-3-[3-[3-methoxy-4-[(methoxycarbonyl)methoxy]phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone [Compound No. (8a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.20 (s, 3H), 3.71 (s, 3H), 3.85 (s, 3H), 4.87 (s, 2H), 5.86 (s, 1H), 6.98 (d, 1H, J=8.1 Hz), 7.25-7.30 (2H), 7.78 (d, 1H, J=14.6 Hz), 8.42 (d, 1H, J=17.3 Hz), 11.49 (broad s, 1H).

Example a-3 Synthesis of the Present Compound [Compound No. (9a)] by Process C

To a solution of 0.42 g of 4-hydroxy-3-[3-[3-[(methoxycarbonyl)methoxy]phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone in 10 ml of methanol was added 10 ml of a 1 N aqueous sodium hydroxide solution. After stirred at room temperature for 6 hours, the solvent was distilled off under reduced pressure, this was acidified with 1 N hydrochloric acid, and precipitated crystals were filtered, washed with water, washed with tetrahydrofuran, and then dried to obtain 0.31 g of 4-hydroxy-3-[3-[3-(carboxymethoxy)phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone [Compound No. (9a)] as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.21 (s, 3H), 4.72 (s, 2H), 5.88 (s, 1H), 7.00-7.04 (m, 1H), 7.22 (s, 1H), 7.29-7.42 (m, 2H), 7.77 (d, 1H, J=15.9 Hz), 8.50 (d, 1H, J=15.9 Hz), 11.59 (s, 1H), 13.00 (broad s, 1H).

Example a-4 Synthesis of the Present Compound [Compound No. (10a)] by Process A

According to the same manner as that of Example a-1 except that 0.53 g of 3-(cyanomethoxy)benzaldehyde was used in place of 3-[(methoxycarbonyl)methoxy]benzaldehyde, 0.43 g of 4-hydroxy-3-[3-[3-(cyanomethoxy)phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone [Compound No. (10a)] was obtained as a yellow crystal.

¹H-NMR (400 MHz, DMSO-d₆) δ (ppm): 2.21 (s, 3H), 5.24 (s, 2H), 5.88 (s, 1H), 7.17 (d, 1H, J=6.4 Hz), 7.37 (s, 1H), 7.42 (d, 1H, J=7.8 Hz), 7.48 (t, 1H, J=7.8 Hz), 7.77 (d, 1H, J=15.9 Hz), 8.51 (d, 1H, J=15.9 Hz), 11.59 (s, 1H).

Example a-5 Synthesis of the Present Compound [Compound No. (11a)] by Process A

According to the same manner as that of Example a-4 except that 0.50 g of 3-acetyl-4-hydroxy-1,6-dimethyl-2(1H)-pyridinone was used in place of 3-acetyl-4-hydroxy-6-methyl-2(1H)-pyridinone, 0.22 g of 4-hydroxy-3-[3-[3-(cyanomethoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (11a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.41 (s, 3H), 3.41 (s, 3H), 5.24 (s, 2H), 6.06 (s, 1H), 7.17 (d, 1H, J=8.1 Hz), 7.35-7.50 (m, 3H), 7.77 (d, 1H, J=15.7 Hz), 8.48 (d, 1H, J=15.7 Hz), 15.95 (s, 1H).

Example a-6 Synthesis of the Present Compound [Compound No. (13a)] by Process A

According to the same manner as that of Example a-1 except that 0.53 g of 4-(cyanomethoxy)benzaldehyde was used in place of 3-[(methoxycarbonyl)methoxy]benzaldehyde, 0.43 g of 4-hydroxy-3-[3-[4-(cyanomethoxy)phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone [Compound No. (13a)] was obtained as a yellow crystal.

¹H-NMR (400 MHz, DMSO-d₆) δ (ppm): 2.20 (s, 3H), 5.25 (s, 2H), 5.87 (s, 1H), 7.17 (d, 2H, J=8.8 Hz), 7.73 (d, 2H, J=8.8 Hz), 7.80 (d, 1H, J=16.2 Hz), 8.46 (d, 1H, J=15.9 Hz), 11.53 (broad s, 1H).

Example 1-7 Synthesis of the Present Compound [Compound No. (18a)] by Process A

According to the same manner as that of Example a-1 except that 1.06 g of 3-(methoxyacetylamino)benzaldehyde was used in place of 3-[(methoxycarbonyl)methoxy]benzaldehyde, 0.74 g of 4-hydroxy-3-[3-[3-(methoxyacetylamino)phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone [Compound No. (18a)] was obtained as a pale yellow crystal.

¹H-NMR (400 MHz, DMSO-d₆) δ (ppm): 2.21 (s, 3H), 3.39 (s, 3H), 4.02 (s, 2H), 5.88 (s, 1H), 7.35-7.45 (2H), 7.75 (d, 1H, J=15.9 Hz), 7.89 (d, 1H, J=7.1 Hz), 8.01 (s, 1H), 8.50 (d, 1H, J=15.6 Hz), 9.95 (s, 1H), 11.54 (broad s, 1H).

Example a-8 Synthesis of the Present Compound [Compound No. (19a)] by Process A

According to the same manner as that of Example a-7 except that 0.49 g of 3-acetyl-4-hydroxy-1,6-dimethyl-2(1H)-pyridinone was used in place of 3-acetyl-4-hydroxy-6-methyl-2(1H)-pyridinone, 0.15 g of 4-hydroxy-3-[3-[3-(methoxyacetylamino)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (19a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.41 (s, 3H), 3.38 (s, 3H), 3.41 (s, 3H), 4.03 (s, 2H), 6.06 (s, 1H), 7.35-7.45 (2H), 7.75 (d, 1H, J=15.7 Hz), 7.79 (d, 1H, J=8.6 Hz), 8.04 (s, 1H), 8.49 (d, 1H, J=15.9 Hz), 9.98 (s, 1H), 16.04 (broad s, 1H).

Example a-9 Synthesis of the Present Compound [Compound No. (20a)] by Process A

According to the same manner as that of Example a-1 except that 0.64 g of 4-(methoxyacetylamino)benzaldehyde was used in place of 3-[(methoxycarbonyl)methoxy]benzaldehyde, 0.67 g of 4-hydroxy-3-[3-[4-(methoxyacetylamino)phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone [Compound No. (20a)] was obtained as a yellow crystal.

¹H-NMR (400 MHz, DMSO-d₆) δ (ppm): 2.20 (s, 3H), 3.38 (s, 3H), 4.03 (s, 2H), 5.87 (s, 1H), 7.65 (d, 2H, J=8.6 Hz), 7.77 (d, 1H, J=16.8 Hz), 7.78 (d, 2H, J=8.3 Hz), 8.47 (d, 1H, J=15.9 Hz), 10.03 (s, 1H), 11.53 (s, 1H).

Example a-10 Synthesis of the Present Compound [Compound No. (22a)] by Process A

According to the same manner as that of Example a-1 except that 1.50 g of 3-[(2-methoxyethyl)aminocarbonylamino]benzaldehyde was used in place of 3-[(methoxycarbonyl)methoxy]benzaldehyde, 0.50 g of 4-hydroxy-3-[3-[3-[(2-methoxyethyl)aminocarbonylamino]phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone [Compound No. (22a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.21 (s, 3H), 3.25-3.45 (4H), 3.33 (s, 3H), 5.88 (s, 1H), 6.24 (t, 1H, J=5.7 Hz), 7.21 (d, 1H, J=7.3 Hz), 7.32 (t, 1H, J=7.8 Hz), 7.50 (d, 1H, J=8.1 Hz), 7.71 (s, 1H), 7.74 (d, 1H, J=15.7 Hz), 8.49 (d, 1H, J=15.9 Hz), 8.73 (s, 1H), 11.54 (s, 1H), 16.49 (s, 1H).

Example a-11 Synthesis of the Present Compound [Compound No. (26a)] by Process A

According to the same manner as that of Example a-1 except that 0.75 g of 3-[(methanesulfonyl)aminocarbonyl]benzaldehyde was used in place of 3-[(methoxycarbonyl)methoxy]benzaldehyde, 0.76 g of 4-hydroxy-3-[3-[3-[(methanesulfonyl)aminocarbonyl]phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone [Compound No. (26a)] was obtained as a yellow crystal.

¹H-NMR (300 MHz, DMSO-d₆) δ (ppm): 2.22 (s, 3H), 3.33 (s, 3H), 5.90 (s, 1H), 7.60 (t, 1H, J=4.5 Hz), 7.84 (d, 1H, J=16.5 Hz), 7.90-8.00 (m, 2H), 8.27 (s, 1H), 8.58 (d, 1H, J=16.5 Hz), 11.61 (broad s, 1H), 12.32 (broad s, 1H).

Example a-12 Synthesis of the Present Compound [Compound No. (28a)] by Process A

According to the same manner as that of Example a-1 except that 5.88 g of 3-[[(methoxycarbonylmethyl)amino]carbonyl]benzaldehyde was used in place of 3-[(methoxycarbonyl)methoxy]benzaldehyde, 4.27 g of 4-hydroxy-3-[3-[3-[[(methoxycarbonylmethyl)amino]carbonyl]phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone [Compound No. (28a)] was obtained as a yellow crystal.

¹H-NMR (400 MHz, DMSO-d₆) δ (ppm): 2.22 (s, 3H), 3.67 (s, 3H), 4.05 (d, 2H, J=5.9 Hz), 5.90 (s, 1H), 7.60 (t, 1H, J=7.8 Hz), 7.83 (d, 1H, J=15.9 Hz), 7.86 (d, 1H, J=8.1 Hz), 7.93 (d, 1H, J=8.3 Hz), 8.18 (s, 1H), 8.56 (d, 1H, J=16.2 Hz), 9.11 (t, 1H, J=5.6 Hz), 11.59 (s, 1H), 13.70 (s, 1H).

Example a-13 Synthesis of the Present Compound [Compound No. (29a)] by Process A

According to the same manner as that of Example a-12 except that 0.50 g of 3-acetyl-4-hydroxy-1,6-dimethyl-2(1H)-pyridinone was used in place of 3-acetyl-4-hydroxy-4-methyl-2(1H)-pyridinone, 0.35 g of 4-hydroxy-3-[3-[3-[[(methoxycarbonylmethyl)amino]carbonyl]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (29a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.41 (s, 3H), 3.42 (s, 3H), 3.68 (s, 3H), 4.05 (d, 2H, J=5.7 Hz), 6.07 (s, 1H), 7.59 (t, 1H, J=7.6 Hz), 7.83 (d, 1H, J=15.7 Hz), 7.87 (d, 1H, J=8.1 Hz), 7.93 (d, 1H, J=8.1 Hz), 8.20 (s, 1H), 8.55 (d, 1H, J=15.9 Hz), 9.13 (t, 1H, J=5.7 Hz), 15.94 (broad s, 1H).

Example a-14 Synthesis of the Present Compound [Compound No. (30a)] by Process A

According to the same manner as that of Example a-1 except that 0.72 g of 4-[[(methoxycarbonylmethyl)amino]carbonyl]benzaldehyde was used in place of 3-[(methoxycarbonyl)methoxy]benzaldehyde, 0.62 g of 4-hydroxy-3-[3-[4-[[(methoxycarbonylmethyl)amino]carbonyl]phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone [Compound No. (30a)] was obtained as a yellow crystal.

¹H-NMR (400 MHz, DMSO-d₆) δ (ppm): 2.22 (s, 3H), 3.67 (s, 3H), 4.03 (d, 2H, J=5.8 Hz), 5.90 (s, 1H), 7.80 (d, 2H, J=8.1 Hz), 7.82 (d, 1H, J=14.2 Hz), 7.94 (d, 2H, J=8.3 Hz), 8.57 (d, 1H, J=15.9 Hz), 9.06 (t, 1H, J=5.6 Hz), 11.59 (broad s, 1H), 13.71 (broad s, 1H).

Example a-15 Synthesis of the Present Compound [Compound No. (31a)] by Process A

According to the same manner as that of Example a-1 except that 0.93 g of 3-[(2-methoxyethyl)aminocarbonyl]benzaldehyde was used in place of 3-[(methoxycarbonyl)methoxy]benzaldehyde, 0.49 g of 4-hydroxy-3-[3-[3-[(2-methoxyethyl)aminocarbonyl]phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone [Compound No. (31a)] was obtained as a yellow crystal.

¹H-NMR (400 MHz, DMSO-d₆) δ (ppm): 2.22 (s, 3H), 3.25-3.32 (2H), 3.33 (s, 3H), 3.40-3.54 (2H), 5.90 (s, 1H), 7.56 (t, 1H, J=7.6 Hz), 7.78-7.88 (2H), 7.90 (d, 1H, J=7.6 Hz), 8.16 (s, 1H), 8.55 (d, 1H, J=15.9 Hz), 8.67 (broad s, 1H), 11.60 (broad s, 1H).

Example a-16 Synthesis of the Present Compound [Compound No. (32a)] by Process A

According to the same manner as that of Example a-15 except that 0.50 g of 3-acetyl-4-hydroxy-1,6-dimethyl-2(1H)-pyridinone was used in place of 3-acetyl-4-hydroxy-6-methyl-2(1H)-pyridinone, 0.14 g of 4-hydroxy-3-[3-[3-[(2-methoxyethyl)aminocarbonyl]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (32a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.42 (s, 3H), 3.25-3.35 (2H), 3.40-3.50 (2H), 3.25 (s, 3H), 3.40 (s, 3H), 6.07 (s, 1H), 7.56 (t, 1H, J=8.1 Hz), 7.82 (d, 1H, J=15.9 Hz), 7.83 (d, 1H, J=7.3 Hz), 7.91 (d, 1H, J=7.8 Hz), 8.18 (s, 1H), 8.54 (d, 1H, J=15.9 Hz), 8.68 (s, 1H), 15.97 (d, 1H).

Example a-17 Synthesis of the Present Compound [Compound No. (33a)] by Process A

According to the same manner as that of Example a-1 except that 0.68 g of 4-[(2-methoxyethyl)aminocarbonyl]benzaldehyde was used in place of 3-[(methoxycarbonyl)methoxy]benzaldehyde, 0.59 g of 4-hydroxy-3-[3-[4-[(2-methoxyethyl)aminocarbonyl]phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone [Compound No. (33a)] was obtained as a yellow crystal.

¹H-NMR (400 MHz, DMSO-d₆) δ (ppm): 2.22 (s, 3H), 3.32 (s, 3H), 3.40-3.50 (4H), 5.90 (s, 1H), 7.77 (d, 2H, J=8.3 Hz), 7.82 (d, 1H, J=15.9 Hz), 7.92 (d, 2H, J=8.3 Hz), 8.56 (d, 1H, J=15.9 Hz), 8.62 (t, 1H, J=4.9 Hz), 11.59 (s, 1H).

Example a-18 Synthesis of the Present Compound [Compound No. (34a)] by Process C

According to the same manner as that of Example a-3 except that 0.60 g of 4-hydroxy-3-[3-[3-[[(methoxycarbonylmethyl)amino]carbonyl]phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone was used in place of 4-hydroxy-3-[3-[3-[(methoxycarbonyl)methoxy]phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone, 0.53 g of 4-hydroxy-3-[3-[3-[[(carboxymethyl)amino]carbonyl]phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone [Compound No. (34a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.21 (s, 3H), 3.95 (d, 2H, J=5.9 Hz), 5.90 (s, 1H), 7.59 (t, 1H, J=7.8 Hz), 7.84 (d, 1H, J=16.2 Hz), 7.86 (d, 1H, J=5.9 Hz), 7.93 (d, 1H, J=7.6 Hz), 8.18 (s, 1H), 8.57 (d, 1H, J=15.9 Hz), 8.99 (t, 1H, J=5.4 Hz), 11.60 (s, 1H), 12.63 (broad s, 1H), 16.36 (s, 1H).

Example a-19 Synthesis of the Present Compound [Compound No. (35a)] by Process A

According to the same manner as that of Example a-1 except that 0.30 g of 3-[(cyanomethyl)aminocarbonyl]benzaldehyde was used in place of 3-[(methoxycarbonyl)methoxy]benzaldehyde, 0.32 g of 4-hydroxy-3-[3-[3-[(cyanomethyl)aminocarbonyl]phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone [Compound No. (35a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.22 (s, 3H), 4.35 (d, 1H, J=5.1 Hz), 5.89 (s, 1H), 7.61 (t, 1H, J=7.6 Hz), 7.83 (d, 1H, J=16.2 Hz), 7.85-7.95 (2H), 8.18 (s, 1H), 8.56 (d, 1H, J=16.2 Hz), 9.36 (t, 1H, J=5.1 Hz), 11.60 (broad s, 1H), 16.30 (broad s, 1H)

Example a-20 Synthesis of the Present Compound [Compound No. (36a)] by Process A

According to the same manner as that of Example a-1 except that 9.45 g of 3-acetyl-4-hydroxy-1,6-dimethyl-2(1H)-pyridinone was used in place of 3-acetyl-4-hydroxy-6-methyl-2(1H)-pyridinone, 7.07 g of 4-hydroxy-3-[3-[3-[(methoxycarbonyl)methoxy]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (36a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.40 (s, 3H), 3.40 (s, 3H), 3.71 (s, 3H), 4.91 (s, 2H), 6.05 (s, 1H), 6.93-6.98 (m, 1H), 7.19 (s, 1H), 7.28-7.40 (m, 2H), 7.81 (d, 1H, J=15.6 Hz), 8.55 (d, 1H, J=16.0 Hz), 16.00 (broad s, 1H)

Example a-21 Synthesis of the Present Compound [Compound No. (37a)] by Process C

According to the same manner as that of Example a-3 except that 40.00 g of 4-hydroxy-3-[3-[3-[(methoxycarbonyl)methoxy]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone was used in place of 4-hydroxy-3-[3-[3-[(methoxycarbonyl)methoxy]phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone, 38.20 g of 4-hydroxy-3-[3-[3-(carboxymethoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (37a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.40 (s, 3H), 3.39 (s, 3H), 4.73 (s, 2H), 6.04 (s, 1H), 7.01 (d, 1H, J=7.8 Hz), 7.22 (s, 1H), 7.28-7.38 (m, 2H), 7.74 (d, 1H, J=16.2 Hz), 8.46 (d, 1H, J=15.7 Hz), 16.01 (s, 1H)

Example a-22 Synthesis of the Present Compound [Compound No. (38a)] by Process A

According to the same manner as that of Example a-1 except that 4.29 g of 3-[(dimethylaminocarbonyl)methoxy]benzaldehyde was used in place of 3-[(methoxycarbonyl)methoxy]benzaldehyde, and 3.40 g of 3-acetyl-4-hydroxy-1,6-dimethyl-2(1H)-pyridinone was used in place of 3-acetyl-4-hydroxy-6-methyl-2(1H)-pyridinone, 1.09 g of 4-hydroxy-3-[3-[3-[(dimethylaminocarbonyl)methoxy]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (38a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.41 (s, 3H), 2.87 (s, 3H), 3.02 (s, 3H), 3.40 (s, 3H), 4.87 (s, 2H), 6.05 (s, 1H), 7.00-7.03 (m, 1H), 7.22 (s, 1H), 7.28-7.40 (m, 2H), 7.75 (d, 1H, J=15.9 Hz), 8.48 (d, 1H, J=15.9 Hz), 16.05 (s, 1H)

Example a-23 Synthesis of the Present Compound [Compound No. (39a)] by Process A

According to the same manner as that of Example a-1 except that 1.22 g of 3-[3-(dimethylamino)propyloxy]benzaldehyde was used in place of 3-[(methoxycarbonyl)methoxy]benzaldehyde, 0.38 g of 4-hydroxy-3-[3-[3-[3-(dimethylamino)propyloxy]phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone [Compound (39a)] was obtained as a yellow crystal.

¹H-NMR (300 MHz, DMSO-d₆) δ (ppm): 1.82-1.91 (m, 2H), 2.16 (s, 6H), 2.21 (s, 3H), 2.38 (t, 2H, J=6.0 Hz), 4.05 (t, 2H, J=6.0 Hz), 5.88 (s, 1H), 7.01-7.05 (m, 1H), 7.22 (s, 1H), 7.26-7.41 (m, 2H), 7.77 (d, 1H, J=18.0 Hz), 8.50 (d, 1H, J=18.0 Hz), 11.68 (s, 1H)

Example a-24 Synthesis of the Present Compound [Compound No. 40a]) by Process A

According to the same manner as that of Example a-1 except that 4.90 g of 3-(2-hydroxyethoxy)benzaldehyde was used in place of 3-[(methoxycarbonyl)methoxy]benzaldehyde, and 4.86 g of 3-acetyl-4-hydroxy-1,6-dimethyl-2(1H)-pyridinone was used in place of 3-acetyl-4-hydroxy-6-methyl-2(1H)-pyridinone, 1.41 g of 4-hydroxy-3-[3-[3-(2-hydroxyethoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (40a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.41 (s, 3H), 3.40 (s, 3H), 3.74 (q, 2H, J=5.1 Hz), 4.04 (t, 2H, J=4.6 Hz), 4.90 (t, 1H, J=5.4 Hz), 6.05 (s, 1H), 7.04 (dd, 1H, J=1.9, 8.1 Hz), 7.25 (s, 1H), 7.28 (d, 1H, J=7.8 Hz), 7.38 (t, 1H, J=7.8 Hz), 7.76 (d, 1H, J=15.9 Hz), 8.49 (d, 1H, J=16.2 Hz), 16.05 (s, 1H)

Example a-25 Synthesis of the Present Compound [Compound No. (41a)] by Process B

To a mixture of 1.41 g of 4-hydroxy-3-[3-[3-(2-hydroxyethoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone and 14 ml of dimethylformamide was added 0.19 g of sodium hydride (60% oily) under ice-cooling. After stirred at room temperature for 1 hour, 1.82 g of iodomethane was added, and this was stirred at room temperature to 42° C. for 5 hours. The reaction solution was neutralized with sodium hydrogen sulfate under ice-cooling, this was concentrated under reduced pressure, and the resulting residue was subjected to silica gel column chromatography to obtain 0.67 g of 4-methoxy-3-[3-[3-(2-hydroxyethoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (41a)] as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.44 (s, 3H), 3.41 (s, 3H), 3.71 (q, 2H, J=5.1 Hz), 3.78 (s, 3H), 4.03 (t, 2H, J=4.6 Hz), 4.86 (t, 1H, J=5.4 Hz), 6.35 (s, 1H), 6.98 (dt, 1H, J=1.9, 6.8 Hz), 7.00 (d, 1H, J=16.2 Hz), 7.26 (d, 1H, J=15.9 Hz), 7.28-7.38 (m, 3H)

Example a-26 Synthesis of the Present Compound [Compound No. (42a)] by Process A

According to the same manner as that of Example a-1 except that 1.00 g of 3-[(2-methoxyethoxy)carbonylamino]benzaldehyde was used in place of 3-[(methoxycarbonyl)methoxy]benzaldehyde, 0.742 g of 3-acetyl-4-hydroxy-1,6-dimethyl-2(1H)-pyridinone was used in place of 3-acetyl-4-hydroxy-6-methyl-2(1H)-pyridinone, and 20 ml of ethanol was used in place of pyridine, 0.96 g of 4-hydroxy-3-[3-[3-[(2-methoxyethoxy)carbonylamino]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (42a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.41 (s, 3H), 3.30 (s, 3H), 3.41 (s, 3H), 3.57-3.63 (2H), 4.20-4.26 (2H), 6.06 (s, 1H), 7.31 (d, 1H, J=7.6 Hz), 7.38 (t, 1H, J=8.1 Hz), 7.54 (d, 1H, J=7.8 Hz), 7.73 (d, 1H, J=15.9 Hz), 7.89 (s, 1H), 8.48 (d, 1H, J=15.9 Hz), 9.92 (s, 1H)

Example a-27 Synthesis of the Present Compound [Compound No. (45a)] by Process A

According to the same manner as that of Example a-5 except that 0.71 g of 3-(2-methylthioethoxy)benzaldehyde was used in place of 3-(cyanomethoxy)benzaldehyde, and 10 ml of methanol was used in place of pyridine, 0.52 g of 4-hydroxy-3-[3-[3-(2-methylthioethoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (45a)] was obtained as a yellow crystal.

¹H-NMR (300 MHz, CDCl₃) δ (ppm): 2.23 (s, 3H), 2.42 (s, 3H), 2.90 (t, 2H, J=6.6 Hz), 3.47 (s, 3H), 4.20 (t, 2H, J=6.6 Hz), 5.87 (s, 1H), 6.89-6.95 (m, 1H), 7.19 (s, 1H), 7.28-7.31 (m, 2H), 7.82 (d, 1H, J=15.9 Hz), 8.55 (d, 1H, J=15.9 Hz)

Example a-28 Synthesis of the Present Compound [Compound No. (48a)] by Process A

According to the same manner as that of Example a-1 except that 0.33 g of 3-(2-hydroxyethoxy)benzaldehyde was used in place of 3-[(methoxycarbonyl)methoxy]benzaldehyde, 0.09 g of 4-hydroxy-3-[3-[3-(2-hydroxyethoxy)phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone [Compound No. (48a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.21 (s, 3H), 3.71-3.76 (m, 2H), 4.04 (t, 2H, J=5.1 Hz), 4.89 (t, 1H, J=5.5 Hz), 5.88 (s, 1H), 7.05 (d, 1H, J=8.0 Hz), 7.24-7.29 (m, 2H), 7.39 (t, 1H, J=8.0 Hz), 7.77 (d, 1H, J=16.1 Hz), 8.51 (d, 1H, J=16.1 Hz), 11.55 (broad s, 1H), 16.43 (broad s, 1H)

Example a-29 Synthesis of the Present Compound [Compound No. (49a)] by Process A

According to the same manner as that of Example a-5 except that 0.36 g of 3-[(2-hydroxyethoxy)methyl]benzaldehyde was used in place of 3-(cyanomethoxy)benzaldehyde, and 5 ml of ethanol was used in place of pyridine, 0.39 g of 4-hydroxy-3-[3-[3-[(2-hydroxyethoxy)methyl]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (49a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.41 (s, 3H), 3.41 (s, 3H), 3.47-3.60 (m, 4H), 4.55 (s, 2H), 4.66 (t, 1H, J=5.5 Hz), 6.06 (s, 1H), 7.41-7.66 (m, 4H), 7.80 (d, 1H, J=15.8 Hz), 8.51 (d, 1H, J=15.8 Hz)

Example a-30 Synthesis of the Present Compound [Compound No. (50a)] by Process A

According to the same manner as that of Example a-5 except that 1.44 g of 3-(3-hydroxypropoxy)benzaldehyde was used in place of 3-(cyanomethoxy)benzaldehyde, 0.90 g of 4-hydroxy-3-[3-[3-(3-hydroxypropoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (50a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 1.84-1.93 (m, 2H), 2.41 (s, 3H), 3.41 (s, 3H), 3.55-3.61 (m, 2H), 4.09 (t, 2H, J=6.5 Hz), 4.56 (t, 1H, J=5.1 Hz), 6.06 (s, 1H), 7.02-7.05 (m, 1H), 7.23-7.41 (m, 3H), 7.76 (d, 1H, J=15.7 Hz), 8.48 (d, 1H, J=15.7 Hz), 16.05 (broad s, 1H)

Example a-31 Synthesis of the Present Compound [Compound No. (51a)] by Process A

According to the same manner as that of Example a-5 except that 0.76 g of 3-(2-methoxyethoxy)benzaldehyde was used in place of 3-(cyanomethoxy)benzaldehyde, and 30 ml of methanol was used in place of pyridine, 0.04 g of 4-hydroxy-3-[3-[3-(2-methoxyethoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (51a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, pyridine-d₅) δ (ppm): 2.02 (s, 3H), 3.22 (s, 3H), 3.24 (s, 3H), 3.56-3.60 (m, 2H), 4.01-4.04 (m, 2H), 5.82 (s, 1H), 6.95-6.98 (m, 1H), 7.11-7.35 (m, 3H), 8.06 (d, 1H, J=15.9 Hz), 8.97 (d, 1H, J=15.9 Hz)

Example a-32 Synthesis of the Present Compound [Compound No. (54a)] by Process E

According to the same manner as that of Example a-5 except that 0.84 g of 3-hydroxybenzaldehyde was used in place of 3-(cyanomethoxy)benzaldehyde, and 20 ml of methanol was used in place of pyridine, 1.04 g of 4-hydroxy-3-[3-(3-hydroxyphenyl)-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone was obtained as a yellow crystal.

¹H-NMR (300 MHz, DMSO-d₆) δ (ppm): 2.40 (s, 3H), 3.40 (s, 3H), 6.05 (s, 1H), 6.84-6.88 (m, 1H), 7.10-7.17 (m, 2H), 7.24-7.29 (m, 1H), 7.72 (d, 1H, J=15.0 Hz), 8.48 (d, 1H, J=15.0 Hz), 9.68 (s, 1H)

To a mixture of 200 mg of 4-hydroxy-3-[3-(3-hydroxyphenyl)-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone, 10 ml of tetrahydrofuran, 170 mg of N-(t-butoxycarbonyl)-2-aminoethanol and 276 mg of triphenylphosphine was added dropwise 414 μl of diethyl azodicarboxylate (40% toluene solution), and this was stirred at room temperature for 47 hours. The solvent was distilled off under reduced pressure, and the resulting residue was subjected to silica gel column chromatography to obtain 75 mg of 4-hydroxy-3-[3-[3-[N-(t-butoxycarbonyl)-2-aminoethoxy]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (54a)].

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 1.39 (s, 9H), 2.41 (s, 3H), 3.27-3.37 (m, 2H), 3.41 (s, 3H), 3.99-4.03 (m, 2H), 6.06 (s, 1H), 7.03-7.06 (m, 2H), 7.24-7.42 (m, 3H), 7.76 (d, 1H, J=15.8 Hz), 8.49 (d, 1H, J=15.8 Hz)

Example a-33 Synthesis of the Present Compound [Compound No. (52a)]

To a mixture of 70 mg of 4-hydroxy-3-[3-[3-[N-(t-butoxycarbonyl)-2-aminoethoxy]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone and 7 ml of chloroform was added 23 μl of trimethylsilane iodide, this was stirred at room temperature for 30 minutes, 46 μl of trimethylsilane iodide was further added, and this was stirred at room temperature for 30 minutes. The solvent was distilled off under reduced pressure, and the resulting crystals were filtered, and washed to obtain 25 mg of 4-hydroxy-3-[3-[3-(2-aminoethoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (52a)] as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.42 (s, 3H), 3.26-3.28 (m, 2H), 3.42 (s, 3H), 4.23 (t, 2H, J=5.0 Hz), 6.08 (s, 1H), 7.11 (d, 1H, J=8.3 Hz), 7.33 (s, 1H), 7.35 (d, 1H, J=8.3 Hz), 7.44 (dd, 1H, J=8.3, 8.3 Hz), 7.79 (d, 1H, J=15.8 Hz), 7.94 (broad s, 2H), 8.50 (d, 1H, J=15.8 Hz)

Example a-34 Synthesis of the Present Compound [Compound No. (55a)] by Process A

According to the same manner as that of Example a-1 except that 0.58 g of 3-(2-dimethylaminoethoxy)benzaldehyde was used in place of 3-[(methoxycarbonyl)methoxy]benzaldehyde, 0.13 g of 4-hydroxy-3-[3-[3-(2-dimethylaminoethoxy)phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone [Compound No. 55a]) was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.21 (s, 3H), 2.23 (s, 6H), 2.64 (t, 2H, J=5.4 Hz), 4.10 (t, 2H, J=5.4 Hz), 5.88 (s, 1H), 7.03-7.12 (m, 1H), 7.24-7.41 (m, 3H), 7.77 (d, 1H, J=16.2 Hz), 8.51 (d, 1H, J=16.2 Hz), 11.56 (broad s, 1H) 16.42 (broad s, 1H)

Example a-35 Synthesis of the Present Compound [Compound No. (56a)] by Process E

To a solution of 0.58 g of 4-hydroxy-3-[3-(3-hydroxyphenyl)-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone in 10 ml of dimethylformamide was added 0.32 g of sodium hydride (60% oily), and this was stirred at room temperature for 1 hour. To the reaction mixture was added 0.25 g of 2-chloroethyldimethylamine hydrochloride, and this was heated at 60° C. for 4 hours. The solvent was distilled off under reduced pressure, and the precipitated crystals were filtered, washed with t-butyl methyl ether, and dried to obtain 0.21 g of 4-hydroxy-3-[3-[3-(2-dimethylaminoethoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (56a)] as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.23 (s, 6H), 2.41 (s, 3H), 2.64 (t, 2H, J=5.4 Hz), 3.40 (s, 3H), 4.10 (t, 2H, J=5.4 Hz), 6.05 (s, 1H), 7.03-7.06 (m, 1H), 7.24-7.41 (m, 3H), 7.76 (d, 1H, J=16.2 Hz), 8.48 (d, 1H, J=16.2 Hz), 16.02 (broad s, 1H)

Example a-36 Synthesis of the Present Compound [Compound No. (57a)] by Process A

According to the same manner as that of example a-5 except that 3.52 g of 3-(3-dimethylaminopropoxy)benzaldehyde was used in place of 3-(cyanomethoxy)benzaldehyde, 1.47 g of 4-hydroxy-3-[3-[3-(3-dimethylaminopropoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (57a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 1.81-1.91 (m, 2H), 2.15 (s, 6H), 2.34-2.40 (m, 5H), 3.40 (s, 3H), 4.05 (t, 2H, J=6.5 Hz), 6.05 (s, 1H), 7.01-7.04 (m, 1H), 7.22-7.40 (m, 3H), 7.76 (d, 1H, J=16.2 Hz), 8.47 (d, 1H, J=16.2 Hz), 15.98 (broad s, 1H)

Example a-37 (1) Synthesis of the Present Compound [Compound No. (59a)] by Process E

According to the same manner as that of Example a-1 except that 1.64 g of 3-hydroxybenzaldehyde was used in place of 3-[(methoxycarbonyl)methoxy]benzaldehyde, 0.67 g of 4-hydroxy-3-[3-(3-hydroxyphenyl)-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.21 (s, 3H), 5.88 (s, 1H), 6.85-6.88 (m, 1H), 7.11 (d, 1H, J=4.9 Hz), 7.11 (s, 1H), 7.27 (dd, 1H, J=8.1 Hz), 7.72 (d, 1H, J=16.2 Hz), 8.49 (d, 1H, J=16.2 Hz), 9.71 (s, 1H), 11.56 (s, 1H), 16.49 (s, 1H)

To a mixture of 0.66 g of 4-hydroxy-3-[3-(3-hydroxyphenyl)-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone and 10 ml of methanol was added 0.32 g of sodium methoxide, and this was stirred at room temperature. The solvent was distilled off under reduced pressure, 30 ml of 2-propanol was added to the resulting residue, and this is heated to dissolve it, and a solution of 0.36 g of 1,3-propanesultone in 10 ml of 2-propanol was added dropwise under reflux. After heated under reflux for 3 hours and then cooled to room temperature, precipitated crystals were filtered. Recrystallization from methanol-ether afforded 0.05 g of 4-hydroxy-6-methyl-3-[3-[3-(3-sulfopropoxy)phenyl]-1-oxo-2-propenyl]-2(1H)-pyridinone sodium salt [Compound No. (59a)] as a yellow crystal.

Example a-37 (2) Synthesis of the Present Compound [Compound No. (59a)] by Process A

To a solution of 1.32 g of sodium 3-(3-formylphenoxy)-1-propanesulfonate and 0.75 g of 3-acetyl-4-hydroxy-6-methyl-2(1H)-pyridinone in 15 ml of ethanol were added 5 ml of a 1 N aqueous sodium hydroxide solution and 3 ml of dimethylformamide, and this was heated and stirred at 65° C. for 4 hours. After cooled to room temperature, precipitated crystals were filtered, washed with t-butyl methyl ether, and dried to obtain 0.22 g of 4-hydroxy-6-methyl-3-[3-[3-(3-sulfopropoxy)phenyl]-1-oxo-2-propenyl]-2(1H)-pyridinone sodium salt [Compound No. (59a)] as an orange crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 1.90 (s, 3H), 1.96-2.06 (m, 2H), 2.51-2.59 (m, 2H), 4.07 (t, 2H, J=6.5 Hz), 5.15 (s, 1H), 6.85-6.88 (m, 1H), 7.00 (s, 1H), 7.07-7.20 (m, 2H), 7.27 (d, 1H, J=16.2 Hz), 7.88 (d, 1H, J=16.2 Hz), 9.40 (broad s, 1H)

Example a-38 Synthesis of the Present Compound [Compound No. (60a)] by Process B

According to the same manner as that of Example a-25 except that 0.71 g of 4-hydroxy-3-[3-[3-(methoxycarbonylmethoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone was used in place of 4-hydroxy-3-[3-[3-(2-hydroxyethoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone, and 0.28 ml of dimethyl sulfate was used in place of iodomethane, 0.37 g of 4-methoxy-3-[3-[3-(methoxycarbonylmethoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (60a)] was obtained as a pale yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.44 (s, 3H), 3.41 (s, 3H), 3.70 (s, 3H), 3.78 (s, 3H), 4.85 (s, 2H), 6.35 (s, 1H), 6.96-7.04 (m, 2H), 7.24-7.35 (m, 4H)

Example a-39 Synthesis of the Present Compound [Compound No. (61a)]

To a mixed solution of 0.10 g of 4-hydroxy-3-[3-[3-(carboxymethoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone, 3 ml of dimethylformamide and 0.03 g of N-hydroxysuccinimide was added 0.06 g of dicyclohexylcarbodiimide, and this was stirred at room temperature for 8.5 hours. Insolubles were filtered, 0.18 g of 1,9-dihydroxynonane was added to the filtrate, and this was stirred at room temperature overnight. The solvent was distilled off under reduced pressure, and the resulting residue was subjected to high performance liquid chromatography to obtain 0.02 g of 4-hydroxy-3-[3-[3-[(9-hydroxynonyl)oxycarbonylmethoxy]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (61a)] as a yellow crystal.

¹H-NMR (270 MHz, CDCl₃) δ (ppm): 1.28-1.67 (m, 14H), 2.37 (s, 3H), 3.48 (s, 3H), 3.63 (t, 2H, J=6.7 Hz), 4.21 (t, 2H, J=6.7 Hz), 4.67 (s, 2H), 5.89 (s, 1H), 6.93-6.95 (m, 1H), 7.19 (s, 1H), 7.27-7.38 (m, 2H), 7.81 (d, 1H, J=15.7 Hz), 8.55 (d, 1H, J=15.7 Hz)

Example a-40 Synthesis of the Present Compound [Compound No. (62a)] by Process A

According to the same manner as that of Example a-1 except that 1.17 g of 4-[(methoxycarbonyl)methoxy]benzaldehyde was used in place of 3-[(methoxycarbonyl)methoxy]benzaldehyde, 0.84 g of 4-hydroxy-3-[3-[4-[(methoxycarbonyl)methoxy]phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone [Compound No. (62a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.20 (s, 3H), 3.71 (s, 3H) 4.89 (s, 2H), 5.86 (s, 1H), 7.04 (d, 2H, J=10.8 Hz), 7.66 (d, 2H, J=8.1 Hz), 7.80 (d, 1H, J=16.2 Hz), 8.45 (d, 1H, J=16.2 Hz), 11.52 (broad s, 1H), 16.65 (broad s, 1H)

Example a-41 Synthesis of the Present Compound [Compound No. (63a)] by Process A

According to the same manner as that of Example a-1 except that 1.21 g of 3-(carboxymethoxy)benzaldehyde was used in place of 3-[(methoxycarbonyl)methoxy]benzaldehyde, 0.53 g of 4-hydroxy-3-[3-[3-(carboxymethoxy)phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone pyridine salt [Compound No. (63a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.21 (s, 3H), 4.74 (s, 2H), 5.89 (s, 1H), 7.00-7.04 (m, 1H), 7.23 (s, 1H), 7.29-7.74 (m, 4H), 7.77-7.81 (m, 1H), 7.79 (d, 1H, J=16.2 Hz), 8.51 (d, 1H, J=16.2 Hz), 8.56-8.59 (m, 2H), 11.59 (s, 1H)

Example a-42 Synthesis of the Present Compound [Compound No. (64a)] by Process C

According to the same manner as that of Example a-3 except that 0.32 g of 4-methoxy-3-[3-[3-(methoxycarbonylmethoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone was used in place of 4-hydroxy-3-[3-[3-[(methoxycarbonyl)methoxy]phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone, 0.29 g of 4-methoxy-3-[3-[3-(carboxymethoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (64a)] was obtained as a pale yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.44 (s, 3H), 3.40 (s, 3H), 3.78 (s, 3H), 4.73 (s, 2H), 6.35 (s, 1H), 6.94-7.02 (m, 2H), 7.22-7.34 (m, 4H)

Example a-43 Synthesis of the Present Compound [Compound No. (65a)] by Process C

According to the same manner as that of Example a-3 except that 0.65 g of 4-hydroxy-3-[3-[4-[(methoxycarbonyl)methoxy]phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone was used in place of 4-hydroxy-3-[3-[3-[(methoxycarbonyl)methoxy]phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone, 0.47 g of 4-hydroxy-3-[3-[4-(carboxymethoxy)phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone [Compound No. (65a)] was obtained as an orange crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.20 (s, 3H), 4.76 (s, 2H), 5.86 (s, 1H), 7.02 (d, 2H, J=8.9 Hz), 7.66 (d, 2H, J=8.1 Hz), 7.80 (d, 1H, J=16.2 Hz), 8.45 (d, 1H, J=16.2 Hz), 11.53 (s, 1H), 13.09 (broad s, 1H), 16.67 (s, 1H)

Example a-44 Synthesis of the Present Compound [Compound No. (66a)] by Process A

According to the same manner as that of Example a-1 except that 0.39 g of 3-(aminocarbonylmethoxy)benzaldehyde was used in place of 3-[(methoxycarbonyl)methoxy]benzaldehyde, 0.48 g of 4-hydroxy-3-[3-[3-(aminocarbonylmethoxy)phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone [Compound No. (66a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.21 (s, 3H), 4.49 (s, 2H), 5.88 (s, 1H), 7.05 (d, 1H, J=8.4 Hz), 7.28-7.59 (m, 5H), 7.76 (d, 1H, J=15.8 Hz), 8.51 (d, 1H, J=15.8 Hz), 11.56 (broad s, 1H), 16.40 (broad s, 1H)

Example a-45 Synthesis of the Present Compound [Compound No. (67a)] by Process A

According to the same manner as that of Example a-44 except that 1.0 g of 3-acetyl-4-hydroxy-1,6-dimethyl-2(1H)-pyridinone was used in place of 3-acetyl-4-hydroxy-6-methyl-2(1H)-pyridinone, 0.72 g of 4-hydroxy-3-[3-[3-(aminocarbonylmethoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (67a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.41 (s, 3H), 3.41 (s, 3H), 4.49 (s, 2H), 6.06 (s, 1H), 7.06 (d, 1H, J=7.8 Hz), 7.30-7.60 (m, 5H), 7.75 (d, 1H, J=15.9 Hz), 8.48 (d, 1H, J=15.9 Hz), 16.04 (broad s, 1H)

Example a-46 Synthesis of the Present Compound [Compound No. (68a)] by Process B

According to the same manner as that of Example a-25 except that 0.78 g of 4-hydroxy-3-[3-[3-(aminocarbonylmethoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone was used in place of 4-hydroxy-3-[3-[3-(2-hydroxyethoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone, and 0.5 ml of dimethyl sulfate was used in place of iodomethane, 0.18 g of 4-methoxy-3-[3-[3-(aminocarbonylmethoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (68a)] was obtained as a yellow crystal.

¹H-NMR (300 MHz, DMSO-d₆) δ (ppm): 2.44 (s, 3H), 3.41 (s, 3H), 3.78 (s, 3H), 4.47 (s, 2H), 6.35 (s, 1H), 6.96-7.02 (m, 2H), 7.20-7.53 (m, 6H)

Example a-47 Synthesis of the Present Compound [Compound No. (69a)] by Process A

According to the same manner as that of Example a-1 except that 1.48 g of 3-[(dimethylaminocarbonyl)methoxy]benzaldehyde was used in place of 3-[(methoxycarbonyl)methoxy]benzaldehyde, 0.50 g of 4-hydroxy-3-[3-[3-[(dimethylaminocarbonyl)methoxy]phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone [Compound No. (69a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.21 (s, 3H), 2.86 (s, 3H), 3.02 (s, 3H), 4.87 (s, 2H), 5.88 (s, 1H), 7.01 (d, 1H, J=8.1 Hz), 7.21 (s, 1H), 7.30-7.40 (m, 2H), 7.77 (d, 1H, J=16.2 Hz), 8.51 (d, 1H, J=13.5 Hz)

Example a-48 Synthesis of the Present Compound [Compound No. (70a)] by Process B

According to the same manner as that of Example a-25 except that 1.59 g of 4-hydroxy-3-[3-[3-[(dimethylaminocarbonyl)methoxy]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone was used in place of 4-hydroxy-3-[3-[3-(2-hydroxyethoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone, 0.60 g of 4-methoxy-3-[3-[3-[(dimethylaminocarbonyl)methoxy]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (70a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.44 (s, 3H), 2.89 (s, 3H), 3.00 (s, 3H), 3.41 (s, 3H), 3.81 (s, 3H), 4.85 (s, 2H), 6.35 (s, 1H), 6.96-7.02 (m, 2H), 7.22-7.34 (m, 4H)

Example a-49 Synthesis of the Present Compound [Compound No. (71a)] by Process A

According to the same manner as that of Example a-1 except that 2.08 g of 3-bromo-4-(methoxycarbonylmethoxy)benzaldehyde was used in place of 3-[(methoxycarbonyl)methoxy]benzaldehyde, and 15 ml of methanol was used in place of pyridine, 0.40 g of 4-hydroxy-3-[3-[3-bromo-4-(methoxycarbonylmethoxy)phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone [Compound No. (71a)] was obtained as a pale yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.21 (s, 3H), 3.72 (s, 3H), 5.02 (s, 2H), 5.88 (s, 1H), 7.14 (d, 1H, J=8.4 Hz), 7.64-7.71 (m, 1H), 7.75 (d, 1H, J=15.8 Hz), 7.93-7.94 (m, 1H), 8.42 (d, 1H, J=15.8 Hz)

Example a-50 Synthesis of the Present Compound [Compound No. (72a)] by Process A

According to the same manner as that of Example a-49 except that 0.50 g of 3-methyl-4-(methoxycarbonylmethoxy)benzaldehyde was used in place of 3-bromo-4-(methoxycarbonylmethoxy)benzaldehyde, 0.36 g of 4-hydroxy-3-[3-[3-methyl-4-(methoxycarbonylmethoxy)phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone [Compound No. (72a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.20 (s, 3H), 2.24 (s, 3H), 3.71 (s, 3H), 4.92 (s, 2H), 5.86 (s, 1H), 6.95 (d, 1H, J=8.1 Hz), 7.48-7.53 (m, 2H), 7.76 (d, 1H, J=15.5 Hz), 8.42 (d, 1H, J=15.5 Hz), 11.51 (broad s, 1H), 16.69 (broad s, 1H)

Example a-51 Synthesis of the Present Compound [Compound No. (73a)] by Process A

According to the same manner as that of Example a-50 except that 0.36 g of 3-acetyl-4-hydroxy-1,6-dimethyl-2(1H)-pyridinone was used in place of 3-acetyl-4-hydroxy-6-methyl-2(1H)-pyridinone, 0.36 g of 4-hydroxy-3-[3-[3-methyl-4-(methoxycarbonylmethoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (73a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.25 (s, 3H), 2.40 (s, 3H), 3.40 (s, 3H), 3.71 (s, 3H), 4.92 (s, 2H), 6.04 (s, 1H), 6.95 (d, 1H, J=8.6 Hz), 7.49-7.54 (m, 2H), 7.76 (d, 1H, J=15.8 Hz), 8.41 (d, 1H, J=15.8 Hz)

Example a-52 Synthesis of the Present Compound [Compound No. (75a)] by Process A

According to the same manner as that of Example a-5 except that 0.36 g of 3-(2-oxo-propoxy)benzaldehyde was used in place of 3-(cyanomethoxy)benzaldehyde, and 5 ml of ethanol was used in place of pyridine, 0.08 g of 4-hydroxy-3-[3-[3-(2-oxo-propoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (75a)]) was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.18 (s, 3H), 2.41 (s, 3H), 3.41 (s, 3H), 4.88 (s, 2H), 6.06 (s, 1H), 6.99-7.02 (m, 1H), 7.21-7.41 (m, 3H), 7.75 (d, 1H, J=16.1 Hz), 8.47 (d, 1H, J=16.1 Hz)

Example a-53 Synthesis of the Present Compound [Compound No. (76a)] by Process A

According to the same manner as that of Example a-5 except that 0.36 g of 3-[(methoxycarbonylmethylthio)methyl]benzaldehyde was used in place of 3-(cyanomethoxy)benzaldehyde, and 8 ml of methanol was used in place of pyridine, 0.24 g of 4-hydroxy-3-[3-[3-[(methoxycarbonylmethylthio)methyl]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (76a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.41 (s, 3H), 3.27 (s, 2H), 3.41 (s, 3H), 3.63 (s, 3H), 3.88 (s, 2H), 6.06 (s, 1H), 7.41-7.47 (m, 2H), 7.59 (d, 1H, J=6.2 Hz), 7.65 (s, 1H), 7.79 (d, 1H, J=16.2 Hz), 8.52 (d, 1H, J=16.2 Hz), 16.06 (broad s, 1H)

Example a-53-2 Synthesis of the Present Compound [Compound No. (77a)]

To a solution of 0.17 g of 4-hydroxy-3-[3-[3-[(methoxycarbonylmethylthio)methyl]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone in 4 ml of methylene chloride was added 0.076 g of m-chloroperbenzoic acid in portions under ice-cooling. After stirred under ice-cooling, the solvent was distilled off under reduced pressure, water was added to the residue, this was extracted with ethyl acetate, washed with an aqueous sodium bicarbonate solution, and further washed with an aqueous saturated sodium chloride solution. After dried over anhydrous magnesium sulfate, the solvent was distilled off under reduced pressure, and the resulting residue was subjected to silica gel column chromatography to obtain 0.055 g of 4-hydroxy-3-[3-[3-[(methoxycarbonylmethylsulfinyl)methyl]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (77a)] as a yellow crystal.

¹H-NMR (300 MHz, DMSO-d₆) δ (ppm): 2.41 (s, 3H), 3.46 (s, 3H), 3.70 (s, 3H), 3.70-4.10 (2H), 4.15-4.40 (2H), 6.07 (s, 1H), 7.41-7.53 (m, 2H), 7.60-7.70 (m, 2H), 7.80 (d, 1H, J=15.9 Hz), 8.53 (d, 1H, J=15.9 Hz)

Example a-53-3 Synthesis of the Present Compound [Compound No. (78a)]

To a solution of 0.096 g of 4-hydroxy-3-[3-[3-[(methoxycarbonylmethylthio)methyl]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone in 4 ml of methylene chloride was added 0.094 g of m-chloroperbenzoic acid under ice-cooling. After stirred for 3 hours under ice-cooling, the solvent was distilled off under reduced pressure, water was added to the residue, and this was extracted with ethyl acetate, washed with an aqueous sodium bicarbonate solution, and was further washed with an aqueous saturated sodium chloride solution. After dried over anhydrous magnesium sulfate, the solvent was distilled off under reduced pressure, and the resulting residue was washed with diethyl ether and hexane to obtain 0.035 g of 4-hydroxy-3-[3-[3-[(methoxycarbonylmethylsulfonyl)methyl]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (78a)] as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.41 (s, 3H), 3.41 (s, 3H), 3.75 (s, 3H), 4.40 (s, 2H), 4.75 (s, 2H), 6.07 (s, 1H), 7.47-7.56 (m, 2H), 7.72-7.75 (m, 2H), 7.81 (d, 1H, J=16.2 Hz), 8.53 (d, 1H, J=16.2 Hz)

Example a-54 Synthesis of the Present Compound [Compound No. (79a)] by Process B

According to the same manner as that of Example a-25 except that 0.88 g of 4-hydroxy-3-[3-[3-(methoxyacetylamino)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone was used in place of 4-hydroxy-3-[3-[3-(2-hydroxyethoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone, 0.42 g of 4-methoxy-3-[3-[3-(methoxyacetylamino)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (79a)] was obtained as a pale yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.44 (s, 3H), 3.38 (s, 3H), 3.41 (s, 3H), 3.79 (s, 3H), 4.00 (s, 2H), 6.35 (s, 1H), 6.93 (d, 1H, J=15.9 Hz), 7.27-7.39 (m, 3H), 7.69-7.73 (m, 1H), 7.94 (s, 1H), 9.82 (broad s, 1H)

Example a-55 Synthesis of the Present Compound [Compound No. (80a)] by Process B

According to the same manner as that of Example a-25 except that 0.46 g of 4-hydroxy-3-[3-[3-[(2-methoxyethoxy)carbonylamino]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone was used in place of 4-hydroxy-3-[3-[3-(2-hydroxyethoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone, 0.12 g of 4-methoxy-3-[3-[3-[(2-methoxyethoxy)carbonylamino]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (80a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.44 (s, 3H), 3.28 (s, 3H), 3.41 (s, 3H), 3.57 (t, 2H, J=4.6 Hz), 3.78 (s, 3H), 4.20 (t, 2H, J=4.6 Hz), 6.35 (s, 1H), 6.90 (d, 1H, J=16.2 Hz), 7.25-7.47 (m, 4H), 7.73 (s, 1H), 9.83 (broad s, 1H)

Example a-56 Synthesis of the Present Compound [Compound No. (82a)] by Process A

According to the same manner as that of Example a-1 except that 2.55 g of 3-(2-dimethylaminoethylamino)benzaldehyde was used in place of 3-[(methoxycarbonyl)methoxy]benzaldehyde, 1.26 g of 4-hydroxy-3-[3-[3-(2-dimethylaminoethylamino)phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone [Compound No. (82a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.20 (s, 9H), 2.42-2.52 (m, 2H), 3.09-3.16 (m, 2H), 5.65 (t, 1H, J=5.4 Hz), 5.87 (s, 1H), 6.68-6.71 (m, 1H), 6.85-6.88 (m, 2H), 7.17 (t, 1H, J=8.1 Hz), 7.69 (d, 1H, J=16.2 Hz), 8.46 (d, 1H, J=16.2 Hz), 11.52 (broad s, 1H), 16.57 (broad s, 1H)

Example a-57 Synthesis of the Present Compound [Compound No. (83a)] by Process B

According to the same manner as that of Example a-25 except that 0.25 g of 4-hydroxy-3-[3-[3-[(methoxycarbonylmethyl)aminocarbonyl]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone was used in place of 4-hydroxy-3-[3-[3-(2-hydroxyethoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone, and 6 ml of hexamethylphosphoramide was used in place of dimethylformamide, 0.13 g of 4-methoxy-3-[3-[3-[(methoxycarbonylmethyl)aminocarbonyl]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (83a)] was obtained as a pale yellow crystal.

¹H-NMR (300 MHz, DMSO-d₆) δ (ppm): 2.45 (s, 3H), 3.42 (s, 3H), 3.66 (s, 3H), 3.79 (s, 3H), 4.02 (d, 2H, J=5.6 Hz), 6.37 (s, 1H), 7.10 (d, 1H, J=16.1 Hz), 7.42 (d, 1H, J=16.1 Hz), 7.54 (t, 1H, J=7.7 Hz), 7.84-7.91 (m, 2H), 8.16 (s, 1H), 9.08 (t, 1H, J=5.6 Hz)

Example a-58 Synthesis of the Present Compound [Compound No. (84a)] by Process A

According to the same manner as that of Example a-5 except that 1.34 g of 4-[(methoxycarbonylmethyl)aminocarbonyl]benzaldehyde was used in place of 3-(cyanomethoxy)benzaldehyde, 0.94 g of 4-hydroxy-3-[3-[4-[(methoxycarbonylmethyl)aminocarbonyl]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (84a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.41 (s, 3H), 3.41 (s, 3H), 3.67 (s, 3H), 4.04 (d, 2H, J=5.9 Hz), 6.06 (s, 1H), 7.78-8.05 (m, 5H), 8.55 (d, 1H, J=16.2 Hz), 9.07 (t, 1H, J=5.9 Hz), 15.76 (broad s, 1H)

Example a-59 Synthesis of the Present Compound [Compound No. (85a)] by Process B

According to the same manner as that of Example a-25 except that 0.61 g of 4-hydroxy-3-[3-[4-[(methoxycarbonylmethyl)aminocarbonyl]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone was used in place of 4-hydroxy-3-[3-[3-(2-hydroxyethoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone, 0.53 g of 4-methoxy-3-[3-[4-[(methoxycarbonylmethyl)aminocarbonyl]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (85a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.44 (s, 3H), 3.41 (s, 3H), 3.66 (s, 3H), 3.79 (s, 3H), 4.02 (d, 2H, J=5.8 Hz), 6.36 (s, 1H), 7.11 (d, 1H, J=16.1 Hz), 7.40 (d, 1H, J=16.1 Hz), 7.78 (d, 2H, J=8.5 Hz), 7.89 (d, 2H, J=8.5 Hz), 9.03 (t, 1H, J=5.8 Hz)

Example a-60 Synthesis of the Present Compound [Compound No. (86a)] by Process C

According to the same manner as that of Example a-3 except that 0.25 g of 4-hydroxy-3-[3-[3-[(methoxycarbonylmethyl)aminocarbonyl]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone was used in place of 4-hydroxy-3-[3-[3-[(methoxycarbonyl)methoxy]phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone, 0.19 g of 4-hydroxy-3-[3-[3-[(carboxymethyl)aminocarbonyl]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (86a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.42 (s, 3H), 3.42 (s, 3H), 3.96 (d, 2H, J=5.5 Hz), 6.07 (s, 1H), 7.59 (t, 1H, J=7.8 Hz), 7.80-7.95 (m, 3H), 8.20 (s, 1H), 8.55 (d, 1H, J=15.7 Hz), 9.00 (t, 1H, J=5.5 Hz), 15.96 (broad s, 1H)

Example a-61 Synthesis of the Present Compound [Compound No. (87a)] by Process C

According to the same manner as that of Example a-3 except that 0.08 g of 4-methoxy-3-[3-[3-[(methoxycarbonylmethyl)aminocarbonyl]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone was used in place of 4-hydroxy-3-[3-[3-[(methoxycarbonyl)methoxy]phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone, 0.07 g of 4-methoxy-3-[3-[3-[(carboxymethyl)aminocarbonyl]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (87a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.45 (s, 3H), 3.42 (s, 3H), 3.79 (s, 3H), 3.94 (d, 2H, J=5.9 Hz), 6.37 (s, 1H), 7.09 (d, 1H, J=16.3 Hz), 7.41 (d, 1H, J=16.3 Hz), 7.53 (t, 1H, J=7.7 Hz), 7.83-7.91 (m, 2H), 8.16 (s, 1H), 8.96 (t, 1H, J=5.9 Hz)

Example a-62 Synthesis of the Present Compound [Compound No. (88a)] by Process C

According to the same manner as that of Example a-3 except that 0.42 g of 4-hydroxy-3-[3-[4-[(methoxycarbonylmethyl)aminocarbonyl]phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone was used in place of 4-hydroxy-3-[3-[3-[(methoxycarbonyl)methoxy]phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone, 0.28 g of 4-hydroxy-3-[3-[4-[(carboxymethyl)aminocarbonyl]phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone [Compound No. (88a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.22 (s, 3H), 3.94 (d, 2H, J=5.8 Hz), 5.90 (s, 1H), 7.78-7.97 (m, 5H), 8.58 (d, 1H, J=15.7 Hz), 8.92 (t, 1H, J=5.8 Hz), 11.60 (broad s, 1H), 16.33 (broad s, 1H)

Example a-63 Synthesis of the Present Compound [Compound No. (89a)] by Process C

According to the same manner as that of Example a-3 except that 0.10 g of 4-hydroxy-3-[3-[4-[(methoxycarbonylmethyl)aminocarbonyl]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone was used in place of 4-hydroxy-3-[3-[3-[(methoxycarbonyl)methoxy]phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone, 0.06 g of 4-hydroxy-3-[3-[4-[(carboxymethyl)aminocarbonyl]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (89a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.41 (s, 3H), 3.41 (s, 3H), 3.95 (d, 2H, J=5.9 Hz), 6.07 (s, 1H), 7.80 (d, 2H, J=8.4 Hz), 7.82 (d, 1H, J=15.7 Hz), 7.95 (d, 2H, J=8.4 Hz), 8.56 (d, 1H, J=15.7 Hz), 8.94 (t, 1H, J=6.5 Hz), 15.9 (s, 1H)

Example a-64 Synthesis of the Present Compound [Compound No. (90a)] by Process C

To 0.15 g of 4-methoxy-3-[3-[4-(methoxycarbonylmethyl)aminocarbonyl]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone was added 2 ml of 2 N hydrochloric acid, and this was stirred at room temperature for 30 minutes, and at 60° C. for 1 hour. The resulting crude crystals were filtered, washed with tetrahydrofuran, and dried to obtain 0.12 g of 4-methoxy-3-[3-[4-[(carboxymethyl)aminocarbonyl]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (90a)] as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.45 (s, 3H), 3.41 (s, 3H), 3.79 (s, 3H), 3.93 (d, 2H, J=5.9 Hz), 6.36 (s, 1H), 7.10 (d, 1H, J=15.9 Hz), 7.40 (d, 1H, J=15.9 Hz), 7.77 (d, 2H, J=8.2 Hz), 7.90 (d, 2H, J=8.2 Hz), 8.93 (t, 1H, J=5.9 Hz)

Example a-65 Synthesis of the Present Compound [Compound No. (91a)]

A mixture of 0.37 g of 4-hydroxy-3-[3-[3-[(methoxycarbonylmethyl)aminocarbonyl]phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone, 18 mg of ammonium chloride and 4 ml of aqueous concentrated ammonia was stirred at room temperature for 1 hour. Two ml of aqueous concentrated ammonia was added, and this was further stirred for 30 minutes, and concentrated under reduced pressure. The residue was washed with tetrahydrofuran, and the resulting crude crystals were recrystallized from dimethylformamide to obtain 0.11 g of 4-hydroxy-3-[3-[3-[(aminocarbonylmethyl)aminocarbonyl]phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone [Compound No. (91a)] as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.22 (s, 3H), 3.84 (d, 2H, J=5.4 Hz), 5.90 (s, 1H), 7.06 (broad s, 1H), 7.41 (broad s, 1H), 7.58 (t, 1H, J=7.8 Hz), 7.81-7.87 (m, 2H), 7.95 (d, 1H, J=7.8 Hz), 8.20 (s, 1H), 8.57 (d, 1H, J=16.2 Hz), 8.83 (t, 1H, J=5.4 Hz)

Example a-66 Synthesis of the Present Compound [Compound No. (92a)]

According to the same manner as that of Example a-65 except that 3.90 g of 4-hydroxy-3-[3-[3-[(methoxycarbonylmethyl)aminocarbonyl]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone was used in place of 4-hydroxy-3-[3-[3-[(methoxycarbonylmethyl)aminocarbonyl]phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone, 2.42 g of 4-hydroxy-3-[3-[3-[(aminocarbonylmethyl)aminocarbonyl]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (92a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.42 (s, 3H), 3.42 (s, 3H), 3.84 (d, 2H, J=5.7 Hz), 6.07 (s, 1H), 7.06 (broad s, 1H), 7.43 (broad s, 1H), 7.57 (t, 1H, J=7.8 Hz), 7.80-7.86 (m, 2H), 7.95 (d, 1H, J=7.8 Hz), 8.22 (s, 1H), 8.55 (d, 1H, J=16.2 Hz), 8.86 (t, 1H, J=5.7 Hz)

Example a-67 Synthesis of the Present Compound [Compound No. (94a)] by Process A

According to the same manner as that of Example a-1 except that 2.64 g of 2-[(3-formylbenzoyl)amino]succinic acid dimethyl ester was used in place of 3-[(methoxycarbonyl)methoxy]benzaldehyde, 0.36 g of 4-hydroxy-3-[3-[3-[[1,2-bis(methoxycarbonyl)ethyl]aminocarbonyl]phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone [Compound No. (94a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.22 (s, 3H), 2.81-3.02 (m, 2H), 3.64 (s, 3H), 3.66 (s, 3H), 4.85-4.88 (m, 1H), 5.89 (s, 1H), 7.59 (t, 1H, J=8.1 Hz), 7.81-7.92 (m, 3H), 8.15 (s, 1H), 8.56 (d, 1H, J=16.2 Hz), 9.07 (d, 1H, J=8.1 Hz), 11.60 (s, 1H), 16.35 (s, 1H)

Example a-68 Synthesis of the Present Compound [Compound No. (95a)] by Process C

According to the same manner as that of Example a-3 except that 0.28 g of 4-hydroxy-3-[3-[3-[[1,2-bis(methoxycarbonyl)ethyl]aminocarbonyl]phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone was used in place of 4-hydroxy-3-[3-[3-[(methoxycarbonyl)methoxy]phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone, 0.21 g of 4-hydroxy-3-[3-[3-[(1,2-dicarboxyethyl)aminocarbonyl]phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone [Compound No. (95a)] was obtained as an orange crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.22 (s, 3H), 2.67-2.91 (m, 2H), 4.77-4.82 (m, 1H), 5.89 (s, 1H), 7.59 (t, 1H, J=8.1 Hz), 7.81-7.93 (m, 3H), 8.16 (s, 1H), 8.56 (d, 1H, J=15.6 Hz), 8.89 (d, 1H, J=8.1 Hz), 11.60 (s, 1H), 12.62 (broad s, 2H), 16.35 (s, 1H)

Example a-69 Synthesis of the Present Compound [Compound No. (96a)]

According to the same manner as that of Example a-5 except that 0.91 g of 3-formylbenzoic acid was used in place of 3-(cyanomethoxy)benzaldehyde, 1.12 g of 4-hydroxy-3-[3-(3-carboxyphenyl)-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.42 (s, 3H), 3.42 (s, 3H), 6.07 (s, 1H), 7.59 (t, 1H, J=7.6 Hz), 7.85 (d, 1H, J=15.5 Hz), 7.91 (d, 1H, J=7.6 Hz), 8.00 (d, 1H, J=7.6 Hz), 8.26 (s, 1H), 8.57 (d, 1H, J=15.5 Hz)

To a mixed solution of 1.57 g of 4-hydroxy-3-[3-(3-carboxyphenyl)-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone, 50 ml of dimethylformamide and 0.58 g of N-hydroxysuccinimide was added a solution of 1.03 g of dicyclohexylcarbodiimide in 5 ml of dimethylformamide, and this was stirred at room temperature overnight. Insolubles were filtered, 0.33 ml of ethanolamine was added to the filtrate, and this was stirred at room temperature for 2.5 hours. To the residue obtained by concentration under reduced pressure was added 30 ml of methanol, and this was heated under reflux. After ice-cooling, crystals were filtered, washed with methanol, and dried to obtain 0.61 g of 4-hydroxy-3-[3-[3-[(2-hydroxyethyl)aminocarbonyl]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (96a)] as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.42 (s, 3H), 3.32-3.39 (m, 2H), 3.42 (s, 3H), 3.50-3.57 (m, 2H), 4.74 (t, 1H, J=5.4 Hz), 6.07 (s, 1H), 7.56 (t, 1H, J=7.6 Hz), 7.80-7.93 (m, 3H), 8.18 (s, 1H), 8.51-8.58 (m, 2H), 15.97 (broad s, 1H)

Example a-70 Synthesis of the Present Compound [Compound No. (97a)] by Process B

According to the same manner as that of Example a-25 except that 0.17 g of 4-hydroxy-3-[3-[3-[(2-hydroxyethyl)aminocarbonyl]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone was used in place of 4-hydroxy-3-[3-[3-(2-hydroxyethoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone, 0.08 g of 4-methoxy-3-[3-[3-[(2-hydroxyethyl)aminocarbonyl]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (97a)] was obtained as a yellow crystal.

¹H-NMR (300 MHz, DMSO-d₆) δ (ppm): 2.45 (s, 3H), 3.32-3.36 (m, 2H), 3.42 (s, 3H), 3.49-3.54 (m, 2H), 3.79 (s, 3H), 4.74 (t, 1H, J=5.7 Hz), 6.37 (s, 1H), 7.08 (d, 1H, J=16.1 Hz), 7.40 (d, 1H, J=16.1 Hz), 7.53 (t, 1H, J=7.8 Hz), 7.81 (d, 1H, J=7.8 Hz), 7.88 (d, 1H, J=7.8 Hz), 8.13 (s, 1H), 8.55 (t, 1H, J=5.6 Hz)

Example a-71 Synthesis of the Present Compound [Compound No. (98a)] by Process B

According to the same manner as that of Example a-25 except that 0.76 g of 4-hydroxy-3-[3-[3-[(2-methoxyethyl)aminocarbonyl]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone was used in place of 4-hydroxy-3-[3-[3-(2-hydroxyethoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone, 0.24 g of 4-methoxy-3-[3-[3-[(2-methoxyethyl)aminocarbonyl]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (98a)] was obtained as a pale yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.44 (s, 3H), 3.27 (s, 3H), 3.42-3.48 (m, 7H), 3.79 (s, 3H), 6.36 (s, 1H), 7.08 (d, 1H, J=16.1 Hz), 7.40 (d, 1H, J=16.1 Hz), 7.50 (t, 1H, J=7.8 Hz), 7.81 (d, 1H, J=7.8 Hz), 7.88 (d, 1H, J=7.8 Hz), 8.13 (s, 1H), 8.62-8.64 (m, 1H)

Example a-72 Synthesis of the Present Compound [Compound No. (99a)] by Process A

According to the same manner as that of Example a-1 except that 1.59 g of 6-formyl-2-[(2-methoxyethyl)aminocarbonyl]pyridine was used in place of 3-[(methoxycarbonyl)methoxy]benzaldehyde, and 30 ml of ethanol was used in place of pyridine, 0.31 g of 4-hydroxy-3-[3-[6-(2-methoxyethyl)aminocarbonyl-2-pyridinyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone (Compound No. (99a)] was obtained as a yellow crystal.

¹H-NMR (300 MHz, CDCl₃) δ (ppm): 2.29 (s, 3H), 3.41 (s, 3H), 3, 59-3.73 (m, 4H), 5.85 (s, 1H), 7.71-7.90 (m, 3H), 8.18 (d, 1H, J=7.5 Hz), 8.43 (m, 1H), 8.85 (d, 1H, J=15.6 Hz), 10.95 (broad s, 1H)

Example a-73 Synthesis of the Present Compound [Compound No. (100a)] by Process A

According to the same manner as that of Example a-5 except that 0.59 g of 3-[(methanesulfonyl)aminocarbonyl]benzaldehyde was used in place of 3-(cyanomethoxy)benzaldehyde, 0.27 g of 4-hydroxy-3-[3-[3-[(methanesulfonyl)aminocarbonyl]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (100a)] was obtained as a pale yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.42 (s, 3H), 3.40 (s, 3H), 3.42 (s, 3H), 6.08 (s, 1H), 7.62 (t, 1H, J=7.8 Hz), 7.83 (d, 1H, J=15.9 Hz), 7.94-8.00 (m, 2H), 8.29 (s, 1H), 8.56 (d, 1H, J=15.9 Hz), 15.92 (broad s, 1H)

Example a-74 Synthesis of the Present Compound [Compound No. (101a)] by Process A

According to the same manner as that of Example a-1 except that 1.54 g of 3-[(2-methoxyethyl)aminosulfonyl]benzaldehyde was used in place of 3-[(methoxycarbonyl)methoxy]benzaldehyde, 0.80 g of 4-hydroxy-3-[3-[3-[(2-methoxyethyl)aminosulfonyl]phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone [Compound No. (101a)] was obtained as a pale yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.22 (s, 3H), 2.88-2.98 (m, 2H), 3.14 (s, 3H), 3.28-3.40 (m, 2H), 5.90 (s, 1H), 7.66-7.95 (m, 5H), 8.11 (s, 1H), 8.57 (d, 1H, J=15.9 Hz), 11.61 (broad s, 1H), 16.26 (broad s, 1H)

Example a-75 Synthesis of the Present Compound [Compound No. (102a)] by Process A

According to the same manner as that of Example a-74 except that 1.39 g of 3-acetyl-4-hydroxy-1,6-dimethyl-2(1H)-pyridinone was used in place of 3-acetyl-4-hydroxy-6-methyl-2(1H)-pyridinone, 0.67 g of 4-hydroxy-3-[3-[3-[(2-methoxyethyl)aminosulfonyl]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (102a)] was obtained as a pale yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.42 (s, 3H), 2.92-2.98 (m, 2H), 3.14 (s, 3H), 3.28-3.33 (m, 2H), 3.41 (s, 3H). 6.08 (s, 1H), 7.66-7.96 (m, 5H), 8.12 (s, 1H), 8.55 (d, 1H, J=15.7 Hz), 15.88 (broad s, 1H)

Example a-76 Synthesis of the Present Compound [Compound No. (103a)] by Process A

According to the same manner as that of Example a-5 except that 0.54 g of 3-(methoxyaminocarbonyl)benzaldehyde was used in place of 3-(cyanomethoxy)benzaldehyde, and 5 ml of tetrahydrofuran was used in place of pyridine, 0.27 g of 4-hydroxy-3-[3-[3-(methoxyaminocarbonyl)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (103a)] was obtained as a yellow crystal.

¹H-NMR (300 MHz, DMSO-d₆) δ (ppm): 2.42 (s, 3H), 3.42 (s, 3H), 3.74 (s, 3H), 6.07 (s, 1H), 7.57 (t, 1H, J=7.5 Hz), 7.73-7.88 (m, 3H), 8.08 (s, 1H), 8.54 (d, 1H, J=18.0 Hz), 11.91 (broad s, 1H)

Example a-77 Synthesis of the Present Compound [Compound No. (104a)] by Process A

According to the same manner as that of Example a-76 except that 0.62 g of 3-(allyloxyaminocarbonyl)benzaldehyde was used in place of 3-(methoxyaminocarbonyl)benzaldehyde, 0.26 g of 4-hydroxy-3-[3-[3-(allyloxyaminocarbonyl)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (104a)] was obtained as a pale yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.42 (s, 3H), 3.42 (s, 3H), 4.44 (d, 2H, J=6.5 Hz), 5.27-5.41 (m, 2H), 5.95-6.03 (m, 1H), 6.07 (s, 1H), 7.57 (t, 1H, J=7.8 Hz), 7.78-7.88 (m, 3H), 8.07 (s, 1H), 8.54 (d, 1H, J=16.2 Hz), 11.83 (broad s, 1H)

Example a-78 Synthesis of the Present Compound [Compound No. (106a)] by Process A

According to the same manner as that of Example a-1 except that 0.74 g of 3-(2-cyanoethyl)benzaldehyde was used in place of 3-[(methoxycarbonyl)methoxy]benzaldehyde, 0.93 g of 3-acetyl-4-hydroxy-1,6-dimethyl-2(1H)-pyridinone was used in place of 3-acetyl-4-hydroxy-6-methyl-2(1H)-pyridinone, and 8 ml of ethanol was used in place of pyridine, 0.70 g of 4-hydroxy-3-[3-[3-(2-cyanoethyl)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (106a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, CDCl₃) δ (ppm): 2.38 (s, 3H), 2.65 (t, 2H, J=7.6 Hz), 3.00 (t, 2H, J=7.6 Hz), 3.49 (s, 3H), 5.90 (s, 1H), 7.25-7.65 (m, 4H), 7.85 (d, 1H, J=15.9 Hz), 8.59 (d, 1H, J=15.9 Hz), 13.74 (s, 1H)

Example a-79 Synthesis of the Present Compound [Compound No. (107a)] by Process A

According to the same manner as that of Example a-78 except that 0.222 g of 3-[(tetrahydropyran-4-ylidene)methyl]benzaldehyde was used in place of 3-(2-cyanoethyl)benzaldehyde, 0.061 g of 4-hydroxy-3-[3-[3-[(tetrahydropyran-4-ylidene)methyl]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone (Compound No. (107a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.34-2.50 (m, 7H), 3.40 (s, 3H), 3.59 (t, 2H, J=5.4 Hz), 3.69 (t, 2H, J=5.4 Hz), 6.06 (s, 1H), 6.40 (s, 1H), 7.31 (d, 1H, J=7.8 Hz), 7.44 (t, 1H, J=7.8 Hz), 7.52 (s, 1H), 7.56 (d, 1H, J=8.1 Hz), 7.80 (d, 1H, J=16.2 Hz), 8.51 (d, 1H, J=16.2 Hz), 16.08 (broad s, 1H)

Example a-80 Synthesis of the Present Compound [Compound No. (108a)] by Process A

According to the same manner as that of Example a-78 except that 0.155 g of 3-(2-cyanoethenyl)benzaldehyde was used in place of 3-(2-cyanoethyl)benzaldehyde, 0.165 g of 4-hydroxy-3-[3-[3-(2-cyanoethenyl)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (108a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.41 (s, 3H), 3.41 (s, 3H), 6.06 (s, 1H), 6.57 (d, 1H, J=16.5 Hz), 7.55 (t, 1H, J=7.6 Hz), 7.70-7.80 (m, 4H), 7.96 (s, 1H), 8.49 (d, 1H, J=16.2 Hz)

Example a-81 Synthesis of the Present Compound [Compound No. (109a)] by Process A

According to the same manner as that of Example a-78 except that 5.7 g of 3-(3-hydroxy-3-methyl-1-butynyl)benzaldehyde was used in place of 3-(2-cyanoethyl)benzaldehyde, and 85 ml of methanol was used in place of ethanol, 1.18 g of 4-hydroxy-3-[3-[3-(3-hydroxy-3-methyl-1-butynyl)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (109a)] was obtained as a pale yellow crystal.

¹H-NMR (400 MHz, DMSO-d₆) δ (ppm): 1.49 (s, 6H), 2.41 (s, 3H), 3.41 (s, 3H), 5.56 (broad s, 1H), 6.07 (s, 1H), 7.47-7.50 (m, 2H), 7.69-7.70 (m, 2H), 7.77 (d, 1H, J=15.9 Hz), 8.49 (d, 1H, J=15.9 Hz)

Example a-82 Synthesis of the Present Compound [Compound No. 110a]) by Process A

According to the same manner as that of Example a-78 except that 0.20 g of methyl 3-(3-formylphenyl)acrylate was used in place of 3-(2-cyanoethyl)benzaldehyde, and 7.5 ml of methanol was used in place of ethanol, 0.032 g of 4-hydroxy-3-[3-[3-(2-methoxycarbonylethenyl)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (110a)] was obtained as a pale yellow crystal.

¹H-NMR (300 MHz, DMSO-d₆) δ (ppm): 2.41 (s, 3H), 3.41 (s, 3H), 3.75 (s, 3H), 6.06 (s, 1H), 6.74 (d, 1H, J=18.0 Hz), 7.50-7.55 (m, 1H), 7.78 (d, 1H, J=18.0 Hz), 7.81 (d, 1H, J=15.0 Hz), 7.75-7.82 (m, 3H), 8.13 (s, 1H), 8.51 (d, 1H, J=15.0 Hz)

Example a-83 Synthesis of the Present Compound [Compound No. (111a) by Process A

According to the same manner as that of Example a-78 except that 0.49 g of 3-allyloxybenzaldehyde was used in place of 3-(2-cyanoethyl)benzaldehyde, 0.58 g of 4-hydroxy-3-[3-(3-allyloxyphenyl)-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (111a)] was obtained as a pale yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.41 (s, 3H), 3.41 (s, 3H), 4.63 (d, 1H, J=5.1 Hz), 5.27-5.47 (m, 2H), 6.00-6.14 (m, 1H), 6.07 (s, 1H), 7.04-7.08 (m, 1H), 7.26-7.42 (m, 3H), 7.76 (d, 1H, J=15.9 Hz), 8.48 (d, 1H, J=15.9 Hz)

Example a-84 Synthesis of the Present Compound [Compound No. (112a)] by Process A

According to the same manner as that of Example a-5 except that 0.41 g of 3-[(methoxycarbonyl)carbonylamino]benzaldehyde was used in place of 3-(cyanomethoxy)benzaldehyde, and 8 ml of methanol was used in place of pyridine, 0.33 g of 4-hydroxy-3-[3-[3-[(methoxycarbonyl)carbonylamino]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (112a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.41 (s, 3H), 3.41 (s, 3H), 3.87 (s, 3H), 6.07 (s, 1H), 7.45-7.48 (m, 2H), 7.76 (d, 1H, J=15.7 Hz), 7.80-7.90 (m, 1H), 8.13 (s, 1H), 8.52 (d, 1H, J=15.4 Hz), 11.01 (s, 1H)

Example a-85 Synthesis of the Present Compound [Compound No. (113a)] by Process A

According to the same manner as that of Example a-5 except that 0.50 g of 3-(methoxyiminomethyl)benzaldehyde was used in place of 3-(cyanomethoxy)benzaldehyde, and 7 ml of methanol was used in place of pyridine, 0.41 g of 4-hydroxy-3-[3-[3-(methoxyiminomethyl)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (113a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.41 (s, 3H), 3.37 (s, 3H), 3.93 (s, 3H), 6.06 (s, 1H), 7.49-7.58 (m, 1H), 7.68-7.92 (m, 3H), 7.79 (d, 1H, J=16.2 Hz), 8.31 (s, 1H), 8.50 (d, 1H, J=16.2 Hz), 14.04 (s, 1H)

Example a-86 Synthesis of the Present Compound [Compound No. (114a)]

According to the same manner as that of Example a-5 except that 7.50 g of 3-(tert-butoxycarbonylamino)benzaldehyde was used in place of 3-(cyanomethoxy)benzaldehyde, and 85 ml of methanol was used in place of pyridine, 4.57 g of 4-hydroxy-3-[3-[3-(tert-butoxycarbonylamino)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone was obtained as a yellow crystal.

¹H-NMR (400 MHz, DMSO-d₆) δ (ppm): 1.50 (s, 9H), 2.41 (s, 3H), 3.41 (s, 3H), 6.06 (s, 1H), 7.29 (d, 1H, J=7.6 Hz), 7.35 (dd, 1H, J=7.6 Hz, 7.6 Hz), 7.55 (d, 1H, J=7.6 Hz), 7.73 (d, 1H, J=15.9 Hz), 7.85 (s, 1H), 8.47 (d, 1H, J=15.8 Hz), 9.55 (broad s, 1H)

To a solution of 2.00 g of 4-hydroxy-3-[3-[3-(tert-butoxycarbonylamino)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone in 200 ml of chloroform was added 2.96 g of iodotrimethylsilane at room temperature, and this was stirred at room temperature overnight. The solvent was distilled off under reduced pressure, and the residue was washed with 60 ml of chloroform to obtain 1.64 g of 4-hydroxy-3-[3-(3-aminophenyl)-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone as a yellow crystal.

¹H-NMR (400 MHz, DMSO-d₆) δ (ppm): 2.43 (s, 3H), 3.41 (s, 3H), 6.09 (s, 1H), 7.36-7.38 (m, 1H), 7.55-7.59 (m, 1H), 7.67-7.69 (m, 2H), 7.81 (d, 1H, J=15.9 Hz), 8.55 (d, 1H, J=15.9 Hz)

To a solution of 0.80 g of 4-hydroxy-3-[3-(3-aminophenyl)-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone in 7 ml of pyridine was added 0.34 g of methyl thioisocyanate, and this was stirred at 80° C. for 4 hours. The solvent was distilled off under reduced pressure, and the residue was washed with 30 ml of tetrahydrofuran and 20 ml of ethyl acetate to obtain 0.86 g of 4-hydroxy-3-[3-[(3-methylthioureido)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (114a)] as a yellow crystal.

¹H-NMR (400 MHz, DMSO-d₆) δ (ppm): 2.42 (s, 3H), 2.94 (s, 3H), 3.41 (s, 3H), 6.07 (s, 1H), 7.39-7.45 (m, 2H), 7.53-7.55 (m, 1H), 7.71 (broad s, 1H), 7.78 (d, 1H, J=16.1 Hz), 8.50 (d, 1H, J=16.1 Hz), 9.74 (broad s, 1H)

Example a-87 Synthesis of the Present Compound [Compound No. (115a)]

To a solution of 0.40 g of 4-hydroxy-3-[3-[(3-methylthioureido)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone in 7 ml of dimethylformamide was added 0.044 g of sodium hydride (60% oily) at room temperature, and this was stirred for 1 hour. 0.26 g of iodomethane was added thereto, and this was stirred at room temperature overnight. The solvent was distilled off under reduced pressure, and the residue was purified by column chromatography to obtain 0.079 g of yellow oily 4-hydroxy-3-[3-[(2,3-dimethylisothioureido)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (115a)].

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.25 (s, 3H), 2.32 (s, 3H), 2.82 (d, 3H, J=4.3 Hz), 3.40 (s, 3H), 6.05 (s, 1H), 6.59-6.60 (m, 1H), 6.83-6.85 (m, 1H), 7.06 (s, 1H), 7.24-7.34 (m, 2H), 7.75 (d, 1H, J=15.8 Hz), 8.48 (d, 1H, J=15.8 Hz), 16.20 (s, 1H)

Example a-88 Synthesis of the Present Compound [Compound No. (116a)] by Process A

According to the same manner as that of Example a-5 except that 0.62 g of dimethyl 3-formylbenzylphosphonate was used in place of 3-(cyanomethoxy)benzaldehyde, and 5 ml of tetrahydrofuran was used in place of pyridine, 0.27 g of 4-hydroxy-3-[3-[3-(dimethoxyphosphorylmethyl)phenyl]-1-oxo-2-propenyl]-1,4-dimethyl-2(1H)-pyridinone [Compound No. (116a)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.41 (s, 3H), 3.37 (d, 2H, J=22.1 Hz), 3.41 (s, 3H), 3.62 (d, 6H, J=11.1 Hz), 6.06 (s, 1H), 7.35-7.50 (m, 2H), 7.55-7.63 (m, 2H), 7.77 (d, 1H, J=16.2 Hz), 8.51 (d, 1H, J=15.9 Hz), 13.94 (broad s, 1H)

Example b-1 Synthesis of the Present Compound [Compound No. (1b)] by Process B

To a mixture of 0.71 g of 4-hydroxy-3-[3-[3-(methoxycarbonylmethoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone in 5 ml of dimethylformamide was added 0.09 g of sodium hydroxide (60% oily), and this was stirred at room temperature for 1 hour. 0.26 ml of allyl bromide was added, and this was stirred at room temperature overnight. The solvent was distilled off under reduced pressure, 5 ml of tetrahydrofuran was added, insolubles were filtered, and the filtrate was concentrated under reduced pressure. The residue was subjected to silica gel column chromatography to obtain 0.30 g of 4-allyloxy-3-[3-[3-(methoxycarbonylmethoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (1b)] as a pale yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.42 (s, 3H), 3.40 (s, 3H), 3.70 (s, 3H), 4.63-4.65 (m, 2H), 4.86 (s, 2H), 5.17-5.35 (m, 2H), 5.80-6.00 (m, 1H), 6.33 (s, 1H), 6.99-7.05 (m, 2H), 7.25-7.36 (m, 4H)

Example b-2 Synthesis of the Present Compound [Compound No. (2b)] by Process B

According to the same manner as that of Example b-1 except that 0.24 ml of propargyl bromide was used in place of allyl bromide, 0.35 g of 4-propargyloxy-3-[3-[3-(methoxycarbonylmethoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (2b)] was obtained as a pale yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.35 (s, 1H), 2.44 (s, 3H), 3.41 (s, 3H), 3.70 (s, 3H), 4.86 (s, 2H), 4.87 (s, 2H), 6.38 (s, 1H), 6.97-7.03 (m, 2H), 7.26-7.34 (m, 4H)

Example b-3 Synthesis of the Present Compound [Compound No. (3b)] by Process B

According to the same manner as that of Example b-1 except that 0.28 ml of methyl bromoacetate was used in place of allyl bromide, 0.73 g of 4-methoxycarbonylmethoxy-3-[3-[3-(methoxycarbonylmethoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (3b)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.39 (s, 3H), 3.39 (s, 3H), 3.66 (s, 3H), 3.69 (s, 3H), 4.85 (s, 2H), 4.89 (s, 2H), 6.26 (s, 1H), 6.96-7.04 (m, 2H), 7.23-7.39 (m, 4H)

Example b-4 Synthesis of the Present Compound [Compound No. (4b)] by Process C

According to the same manner as that of Example a-64 except that 0.66 g of 4-methoxycarbonylmethoxy-3-[3-[3-(methoxycarbonylmethoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone was used in place of 4-methoxy-3-[3-[3-[(methoxycarbonylmethyl)aminocarbonyl]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone, 0.07 g of 4-carboxymethoxy-3-[3-[3-(carboxymethoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (4b)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.40 (s, 3H), 3.39 (s, 3H), 4.73 (s, 2H), 4.79 (s, 2H), 6.25 (s, 1H), 6.97-7.03 (m, 2H), 7.21-7.42 (m, 4H)

Example b-5 Synthesis of the Present Compound [Compound No. (5b)]

According to the same manner as that of Example a-65 except that 0.80 g of 4-methoxycarbonylmethoxy-3-[3-[3-(methoxycarbonylmethoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone was used in place of 4-hydroxy-3-[3-[3-[(methoxycarbonylmethyl)aminocarbonyl]phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone, 0.74 g of 4-aminocarbonylmethoxy-3-[3-[3-(aminocarbonylmethoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (5b)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.42 (s, 3H), 3.42 (s, 3H), 4.47 (s, 2H), 4.59 (s, 2H), 6.31 (s, 1H), 7.00-7.03 (m, 1H), 7.15-7.54 (m, 9H)

Example b-6 Synthesis of the Present Compound [Compound No. (6b)] by Process B

According to the same manner as that of Example b-1 except that 0.21 ml of bromoacetonitrile was used in place of allyl bromide, 0.76 g of 4-cyanomethoxy-3-[3-[3-(methoxycarbonylmethoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (6b)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.46 (s, 3H), 3.42 (s, 3H), 3.69 (s, 3H), 4.84 (s, 2H), 5.21 (s, 2H), 6.44 (s, 1H), 6.97-7.04 (m, 2H), 7.24-7.36 (m, 4H)

Example b-7 Synthesis of the Present Compound [Compound No. (7b)] by Process B

According to the same manner as that of Example b-1 except that 0.42 ml of 2-bromoethanol was used in place of allyl bromide, 0.02 g of 4-(2-hydroxyethoxy)-3-[3-[3-(carboxymethoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (7b)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.41 (s, 3H), 3.41 (s, 3H), 3.58-3.64 (m, 2H), 4.18 (t, 2H, J=4.9 Hz), 4.86 (s, 2H), 6.06 (s, 1H), 7.00-7.08 (m, 1H), 7.25-7.45 (m, 3H), 7.75 (d, 1H, J=16.1 Hz), 8.47 (d, 1H, J=16.1 Hz)

Example b-8 Synthesis of the Present Compound [Compound No. (8b)] by Process B

According to the same manner as that of Example b-1 except that 0.36 ml of benzyl bromide was used in place of allyl bromide, 0.46 g of 4-benzyloxy-3-[3-[3-(methoxycarbonylmethoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (8b)] was obtained.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.42 (s, 3H), 3.40 (s, 3H), 3.70 (s, 3H), 4.86 (s, 2H), 5.20 (s, 2H), 6.44 (s, 1H), 6.98-7.07 (m, 2H), 7.27-7.39 (m, 9H)

Example b-9 Synthesis of the Present Compound [Compound No. (9b)] by Process C

According to the same manner as that of Example a-64 except that 0.41 g of 4-benzyloxy-3-[3-[3-(methoxycarbonylmethoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone was used in place of 4-methoxy-3-[3-[3-[(methoxycarbonylmethyl)aminocarbonyl]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone, 0.12 g of 4-benzyloxy-3-[3-[3-(carboxymethoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (9b)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.42 (s, 3H), 3.40 (s, 3H), 4.73 (s, 2H), 5.20 (s, 2H), 6.44 (s, 1H), 6.95-7.05 (m, 2H), 7.25-7.36 (m, 9H)

Example b-10 Synthesis of the Present Compound [Compound No. (13b)]

To a solution of 0.71 g of 4-hydroxy-3-[3-[3-(methoxycarbonylmethoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone in 5 ml of dimethylformamide was added 0.090 g of sodium hydride (60% oily), and this was stirred at room temperature for 1 hour. 0.46 g of paratoluenesulfonyl chloride was added thereto, this was stirred at room temperature for 4 hours, then, 0.60 g of propargylamine was added, and this was stirred at room temperature overnight. To the reaction solution was added water, the solvent was distilled off under reduced pressure, and the residue was subjected to silica gel column chromatography to obtain 0.080 g of 4-propargylamino-3-[3-[3-(methoxycarbonylmethoxy)phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (13b)] as a yellow crystal.

¹H-NMR (270 MHz, CDCl₃) δ (ppm): 2.31 (t, 1H, J=2.4 Hz), 2.37 (s, 3H), 3.46 (s, 3H), 3.82 (s, 3H), 4.05 (dd, 2H, J=2.4, 5.7 Hz), 4.67 (s, 2H), 5.79 (s, 1H), 6.88 (d, 1H, J=7.6 Hz), 7.15 (s, 1H), 7.24-7.33 (m, 2H), 7.55 (d, 1H, J=15.5 Hz), 8.26 (d, 1H, J=15.5 Hz), 10.99 (broad s, 1H)

Example b-11 Synthesis of the Present Compound [Compound No. (14b)]

According to the same manner as that of Example b-10 except that 0.86 g of 2-methoxyethylamine was used in place of propargylamine, 0.23 g of 4-(2-methoxyethylamino)-3-[3-[3-[(2-methoxyethylamino)carbonylmethoxy]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (14b)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, CDCl₃) δ (ppm): 2.33 (s, 3H), 3.36 (s, 3H), 3.42-3.65 (m, 8H), 3.44 (s, 3H), 3.46 (s, 3H), 4.53 (s, 2H), 5.73 (s, 1H), 6.84-6.89 (m, 1H), 6.96 (broad s, 1H), 7.20-7.30 (m, 3H), 7.54 (d, 1H, J=15.5 Hz), 8.26 (d, 1H, J=15.5 Hz), 11.00 (broad s, 1H)

Example c-1 Synthesis of the Present Compound [Compound No. (1c)] by Process A

According to the same manner as that of Example a-5 except that 0.20 g of 3-acetyl-1-allyl-4-hydroxy-6-methyl-2(1H)-pyridinone was used in place of 3-acetyl-4-hydroxy-1,6-dimethyl-2(1H)-pyridinone, and 6 ml of ethanol was used in place of pyridine, 0.23 g of 1-allyl-3-[3-[3-(cyanomethoxy)phenyl]-1-oxo-2-propenyl]-4-hydroxy-6-methyl-2(1H)-pyridinone [Compound No. (1c)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.40 (s, 3H), 4.64 (d, 2H, J=5.4 Hz), 4.96-5.18 (m, 2H), 5.24 (s, 1H), 5.87-6.01 (m, 1H), 6.09 (s, 1H), 7.16-7.20 (m, 1H), 7.37-7.51 (m, 3H), 7.78 (d, 1H, J=16.2 Hz), 8.47 (d, 1H, J=16.2 Hz), 16.09 (s, 1H)

Example c-2 Synthesis of the Present Compound [Compound No. (2c)] by Process A

According to the same manner as that of Example c-1 except that 0.56 g of 3-acetyl-4-hydroxy-6-methyl-1-propargyl-2(1H)-pyridinone was used in place of 3-acetyl-1-allyl-4-hydroxy-6-methyl-2(1H)-pyridinone, 0.45 g of 3-[3-[3-(cyanomethoxy)phenyl]-1-oxo-2-propenyl]-4-hydroxy-6-methyl-1-propargyl-2(1H)-pyridinone [Compound No. (2c)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.49 (s, 1H), 2.50 (s, 3H), 4.85 (s, 2H), 5.24 (s, 2H), 6.12 (s, 1H), 7.16-7.20 (m, 1H), 7.39-7.51 (m, 3H), 7.79 (d, 1H, J=16.2 Hz), 8.45 (d, 1H, J=16.2 Hz), 16.06 (broad s, 1H)

Example c-3 Synthesis of the Present Compound [Compound No. (3c)] by Process A

According to the same manner as that of Example c-1 except that 0.60 g of 3-acetyl-4-hydroxy-1-(methoxycarbonylmethyl)-6-methyl-2(1H)-pyridinone was used in place of 3-acetyl-1-allyl-4-hydroxy-6-methyl-2(1H)-pyridinone, 0.20 g of 3-[3-[3-(cyanomethoxy)phenyl]-1-oxo-2-propenyl]-4-hydroxy-1-(methoxycarbonylmethyl)-6-methyl-2(1H)-pyridinone [Compound No. (3c)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, CDCl₃) δ (ppm): 2.32 (s, 3H), 3.82 (s, 3H), 4.75 (s, 2H), 4.81 (s, 2H), 5.94 (s, 1H), 7.01 (d, 1H, J=8.1 Hz), 7.24 (s, 1H), 7.34-7.44 (m, 2H), 7.82 (d, 1H, J=16.2 Hz), 8.58 (d, 1H, J=16.2 Hz), 16.39 (s, 1H)

Example c-4 Synthesis of the Present Compound [Compound No. (4c)]

According to the same manner as that of Example a-3 except that 0.15 g of 3-[3-[3-(cyanomethoxy)phenyl]-1-oxo-2-propenyl]-4-hydroxy-1-(methoxycarbonylmethyl)-6-methyl-2(1H)-pyridinone was used in place of 4-hydroxy-3-[3-[3-[(methoxycarbonyl)methoxy]phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone, 0.13 g of 1-(carboxymethyl)-3-[3-[3-(cyanomethoxy)phenyl]-1-oxo-2-propenyl]-4-hydroxy-6-methyl-2(1H)-pyridinone [Compound No. (4c)] was obtained as an orange crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.35 (s, 3H), 4.72 (s, 2H), 4.74 (s, 2H), 6.12 (s, 1H), 7.02 (d, 1H, J=8.1 Hz), 7.24 (s, 1H), 7.31-7.42 (m, 2H), 7.78 (d, 1H, J=16.2 Hz), 8.41 (d, 1H, J=16.2 Hz), 13.10 (broad s, 1H), 16.21 (s, 1H)

Example c-5 Synthesis of the Present Compound [Compound No. (5c)] by Process A

According to the same manner as that of Example c-1 except that 0.57 g of 3-acetyl-1-(aminocarbonylmethyl)-4-hydroxy-6-methyl-2(1H)-pyridinone was used in place of 3-acetyl-1-allyl-4-hydroxy-6-methyl-2(1H)-pyridinone, 0.44 g of 1-(aminocarbonylmethyl)-3-[3-[3-(cyanomethoxy)phenyl]-1-oxo-2-propenyl]-4-hydroxy-6-methyl-2(1H)-pyridinone [Compound No. (5c)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.32 (s, 3H), 4.62 (s, 2H), 5.23 (s, 2H), 6.08 (s, 1H), 7.17 (d, 1H, J=8.1 Hz), 7.27 (s, 1H), 7.37-7.50 (m, 3H), 7.71 (s, 1H), 7.78 (d, 1H, J=16.2 Hz), 8.45 (d, 1H, J=16.2 Hz), 16.12 (s, 1H)

Example c-6 Synthesis of the Present Compound [Compound No. (6c)] by Process D

To a solution of 0.50 g of 4-hydroxy-3-[3-[3-(cyanomethoxy)phenyl]-1-oxo-2-propenyl]-6-methyl-2(1H)-pyridinone in 6 ml of hexamethylphosphoramide was added 46 mg of sodium hydride (60% oily) under ice-cooling, and this was stirred at room temperature for 1 hour. To the reaction mixture was added 0.33 ml of bromoacetonitrile under ice-cooling, and this was stirred at room temperature for 2 hours. The solvent was distilled off under reduced pressure, and precipitated crystals were filtered, washed with t-butyl methyl ether, and dried to obtain 0.14 g of 1-(cyanomethoxy)-3-[3-[3-(cyanomethoxy)phenyl]-1-oxo-2-propenyl]-4-hydroxy-6-methyl-2(1H)-pyridinone [Compound No. (6c)] as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.47 (s, 3H), 5.10 (s, 2H), 5.24 (s, 2H), 6.21 (s, 1H), 7.19 (d, 1H, J=8.1 Hz), 7.40 (s, 1H), 7.44-7.52 (m, 2H), 7.82 (d, 1H, J=16.2 Hz), 8.42 (d, 1H, J=16.2 Hz), 16.21 (s, 1H)

Example c-7 Synthesis of the Present Compound [Compound No. (7c)] by Process D

According to the same manner as that of Example c-6 except that 0.45 ml of bromoacetone was used in place of bromoacetonitrile, 0.23 g of 3-[3-[3-(cyanomethoxy)phenyl]-1-oxo-2-propenyl]-4-hydroxy-6-methyl-1-(2-oxo-propyl)-2(1H)-pyridinone [Compound No. (7c)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.25 (s, 3H), 2.26 (s, 3H), 4.94 (s, 2H), 5.23 (s, 2H), 6.10 (s, 1H), 7.17 (d, 1H, J=8.1 Hz), 7.36-7.50 (m, 3H), 7.78 (d, 1H, J=16.2 Hz), 8.40 (d, 1H, J=16.2 Hz), 16.11 (s, 1H)

Example c-8 Synthesis of the Present Compound [Compound No. (8c)] by Process D

According to the same manner as that of Example c-6 except that 0.69 g of 2-methoxyethyl p-toluenesulfonate was used in place of bromoacetonitrile, 0.03 g of 3-[3-[3-(cyanomethoxy)phenyl]-1-oxo-2-propenyl]-4-hydroxy-1-(2-methoxyethyl)-6-methyl-2(1H)-pyridinone [Compound No. (8c)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.46 (s, 3H), 3.25 (s, 3H), 3.58 (t, 2H, J=5.4 Hz), 4.12 (t, 2H, J=5.4 Hz), 5.24 (s, 2H), 6.05 (s, 1H), 7.18 (d, 1H, J=5.4 Hz), 7.38 (s, 1H), 7.42-7.51 (m, 2H), 7.77 (d, 1H, J=16.2 Hz), 8.47 (d, 1H, J=16.2 Hz), 16.01 (s, 1H)

Example c-9 Synthesis of the Present Compound [Compound No. (9c)] by Process A

According to the same manner as that of Example c-1 except that 0.31 g of 3-acetyl-1-benzyl-4-hydroxy-6-methyl-2(1H)-pyridinone was used in place of 3-acetyl-1-allyl-4-hydroxy-6-methyl-2(1H)-pyridinone, 0.25 g of 1-benzyl-3-[3-[3-(cyanomethoxy)phenyl]-1-oxo-2-propenyl]-4-hydroxy-6-methyl-2(1H)-pyridinone [Compound No. (9c)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.32 (s, 3H), 5.22 (s, 2H), 5.30 (s, 2H), 6.13 (s, 1H), 7.16 (d, 1H, J=8.1 Hz), 7.24-7.49 (m, 8H), 7.79 (d, 1H, J=16.2 Hz), 8.47 (d, 1H, J=16.2 Hz), 16.02 (broad s, 1H)

Example c-10 Synthesis of the Present Compound [Compound No. (10c)] by Process A

According to the same manner as that of Example c-1 except that 0.75 g of 3-acetyl-4-hydroxy-6-methyl-1-phenyl-2(1H)-pyridinone was used in place of 3-acetyl-1-allyl-4-hydroxy-6-methyl-2(1H)-pyridinone, 0.07 g of 3-[3-[3-(cyanomethoxy)phenyl]-1-oxo-2-propenyl]-4-hydroxy-6-methyl-1-phenyl-2(1H)-pyridinone [Compound No. (10c)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 1.94 (s, 3H), 5.20 (s, 2H), 6.21 (s, 1H), 7.13-7.16 (m, 1H), 7.33-7.58 (m, 8H), 7.79 (d, 1H, J=16.2 Hz), 8.42 (d, 1H, J=16.2 Hz), 16.39 (broad s, 1H)

Example c-11 Synthesis of the Present Compound [Compound No. (11c)] by Process A

According to the same manner as that of Example c-1 except that 0.75 g of 3-acetyl-4-hydroxy-6-methyl-1-(2′-pyridinyl)-2(1H)-pyridinone was used in place of 3-acetyl-1-allyl-4-hydroxy-6-methyl-2(1H)-pyridinone, 0.64 g of 3-[3-[3-(cyanomethoxy)phenyl]-1-oxo-2-propenyl]-4-hydroxy-6-methyl-1-(2′-pyridinyl)-2(1H)-pyridinone [Compound No. (11c)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 1.93 (s, 3H), 5.20 (s, 2H), 6.23 (s, 1H), 7.13-7.16 (m, 1H), 7.34-7.46 (m, 3H), 7.55-7.59 (m, 2H), 7.81 (d, 1H, J=16.2 Hz), 8.04-8.10 (m, 1H), 8.36 (d, 1H, J=16.2 Hz), 8.65 (d, 1H, J=3.0 Hz), 16.45 (broad s, 1H)

Example d-1 Synthesis of the Present Compound [Compound No. (b 1d)]

To a solution of 0.50 g of 4-methoxy-3-[3-[3-[(methoxycarbonyl)methoxy]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone in 15 ml of chloroform was added dropwise a solution of 70 μl of bromine in 7 ml of chloroform under ice-cooling. After stirred for 1 hour under ice-cooling, the solvent was distilled off under reduced pressure, and the resulting residue was subjected to silica gel column chromatography to obtain 0.09 g of 5-bromo-4-hydroxy-3-[3-[3-[(methoxycarbonyl)methoxy]phenyl]-1-oxo-2-propenyl]-1,6-dimethyl-2(1H)-pyridinone [Compound No. (1d)] as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 2.70 (s, 3H), 3.45 (s, 3H), 3.70 (s, 3H), 4.87 (s, 2H), 7.06 (d, 1H, J=6.8 Hz), 7.27 (s, 1H), 7.34-7.44 (m, 2H), 7.85 (d, 1H, J=16.2 Hz), 8.50 (d, 1H, J=16.2 Hz), 17.46 (s, 1H)

Example e-1 Synthesis of the Present Compound [Compound No. (7e)] by Process A

To a mixture of 1.43 g of 3-[(methoxycarbonyl)methoxy]benzaldehyde, 0.50 g of 3-acetyl-4-hydroxy-2(1H)-quinolinone and 6 ml pyridine was added 0.1 ml of piperidine, and this was heated under reflux for 1 hour. After cooled to room temperature, precipitated crystals were filtered, and washed with tetrahydrofuran to obtain 0.63 g of 4-hydroxy-3-[3-[3-[(methoxycarbonyl)methoxy]phenyl]-1-oxo-2-propenyl]-2(1H)-quinolinone [Compound No. (7e)] as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 3.74 (s, 3H), 4.88 (s, 2H), 7.07 (d, 1H, J=7.3 Hz), 7.22-7.46 (m, 5H), 7.67-7.73 (m, 1H), 7.91 (d, 1H, J=16.1 Hz), 8.03 (d, 1H, J=7.6 Hz), 8.63 (d, 1H, J=16.1 Hz), 11.54 (broad s, 1H), 18.00 (broad s, 1H)

Example e-2 Synthesis of the Present Compound [Compound No. (9e)] by Process C

To a mixture of 0.50 g of 4-hydroxy-3-[3-[3-[(methoxycarbonyl)methoxy]phenyl]-1-oxo-2-propenyl]-2(1H)-quinolinone and 15 ml of methanol was added dropwise 15 ml of a 1 N aqueous sodium hydroxide solution under ice-cooling. After stirred for 1 hour under ice-cooling, this was concentrated under reduced pressure. The resulting residue was acidified by addition of 2 N hydrochloric acid under ice-cooling, and precipitated crystals were filtered, and washed with tetrahydrofuran to obtain 0.46 g of 4-hydroxy-3-[3-[3-(carboxymethoxy)phenyl]-1-oxo-2-propenyl]-2(1H)-quinolinone [Compound No. (9e)] as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 4.75 (s, 2H), 7.03-7.06 (m, 1H), 7.22-7.45 (m, 5H), 7.66-7.72 (m, 1H), 7.90 (d, 1H, J=15.8 Hz), 8.03 (d, 1H, J=7.6 Hz), 8.64 (d, 1H, J=15.8 Hz)

Example e-3 Synthesis of the Present Compound [Compound No. (10e)] by Process A

In a mixture of 3000 ml of chloroform and 600 ml of dimethylformamide were dissolved 60.00 g of 3-acetyl-4-hydroxy-2(1H)-quinolinone, 142.20 g of 3-(cyanomethoxy)benzaldehyde and 17.64 g of piperidine, and the solution was heated overnight under reflux while water was removed with a Soxhlet extractor filled with molecular sieves. After cooled to room temperature, precipitated crystals were filtered, and washed with 750 ml of tetrahydrofuran and 900 ml of t-butyl methyl ether to obtain 77.59 g of 4-hydroxy-3-[3-[3-(cyanomethoxy)phenyl]-1-oxo-2-propenyl]-2(1H)-quinolinone [Compound No. (10e)] as a yellow crystal.

¹H-NMR (400 MHz, DMSO-d₆) δ (ppm): 5.21 (s, 2H), 7.16 (dd, 1H, J=2.0, 7.6 Hz), 7.23 (t, 1H, J=7.6 Hz), 7.28 (d, 1H, J=8.4 Hz), 7.38 (s, 1H), 7.40-7.50 (m, 2H), 7.65 (t, 1H, J=7.6 Hz), 7.87 (d, 1H, J=16.0 Hz), 7.99 (d, 1H, J=8.0 Hz), 8.60 (d, 1H, J=16.0 Hz)

Example e-4 Synthesis of the Present Compound [Compound No. (11e)] by Process A

According to the same manner as that of Example e-3 except that 1.00 g of 1-methyl-3-acetyl-4-hydroxy-2(1H)-quinolinone was used in place of 3-acetyl-4-hydroxy-2(1H)-quinolinone, 1.16 g of 1-methyl-4-hydroxy-3-[3-[3-(cyanomethoxy)phenyl]-1-oxo-2-propenyl]-2(1H)-quinolinone [Compound No. (11e)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 3.61 (s, 3H), 5.26 (s, 2H), 7.20 (d, 1H, J=6.5 Hz), 7.21 (t, 1H, J=7.3 Hz), 7.35 (t, 1H, J=7.8 Hz), 7.43 (s, 1H), 7.51 (t, 1H, J=7.3 Hz), 7.63 (d, 1H, J=7.8 Hz), 7.83 (dt, 1H, J=1.4, 8.1 Hz), 7.88 (d, 1H, J=16.5 Hz), 8.16 (dd, 1H, J=1.4, 7.8 Hz), 8.56 (d, 1H, J=16.2 Hz), 17.65 (broad s, 1H)

Example e-5 Synthesis of the Present Compound [Compound No. (13e)] by Process A

According to the same manner as that of Example e-3 except that 1.67 g of 4-(cyanomethoxy)benzaldehyde was used in place of 3-(cyanomethoxy)benzaldehyde, 0.92 g of 4-hydroxy-3-[3-[4-(cyanomethoxy)phenyl]-1-oxo-2-propenyl]-2(1H)-quinolinone [Compound No. (13e)] was obtained as a yellow crystal.

¹H-NMR (400 MHz, DMSO-d₆) δ (ppm): 5.27 (s, 2H), 7.20 (d, 2H, J=8.8 Hz), 7.24-7.26 (m, 1H), 7.31 (d, 1H, J=8.3 Hz), 7.68-7.72 (m, 1H), 7.79 (d, 2H, J=8.8 Hz), 7.96 (d, 1H, J=15.9 Hz), 8.02 (d, 1H, J=8.1 Hz), 8.59 (d, 1H, J=16.1 Hz), 11.51 (s, 1H)

Example e-6 Synthesis of the Present Compound [Compound No. (18e)] by Process A

According to the same manner as that of Example e-3 except that 51.34 g of 3-(methoxyacetylamino)benzaldehyde was used in place of 3-(cyanomethoxy)benzaldehyde, 28.65 g of 4-hydroxy-3-[3-[3-(methoxyacetylamino)phenyl]-1-oxo-2-propenyl]-2(1H)-quinolinone [Compound No. (18e)] was obtained as a yellow crystal.

¹H-NMR (400 MHz, DMSO-d₆) δ (ppm): 3.40 (s, 3H), 4.04 (s, 2H), 7.25 (t, 1H, J=7.8 Hz), 7.33 (d, 1H, J=8.0 Hz), 7.42-7.48 (2H), 7.70 (t, 1H, J=6.8 Hz), 7.80-7.86 (broad s, 1H), 7.89 (d, 1H, J=15.9 Hz), 8.02 (d, 1H, J=7.3 Hz), 8.07 (s, 1H), 8.63 (d, 1H, J=15.6 Hz), 9.99 (s, 1H), 11.50 (s, 1H)

Example e-7 Synthesis of the Present Compound [Compound No. (19e)] by Process A

According to the same manner as that of Example e-6 except that 1.00 g of 1-methyl-3-acetyl-4-hydroxy-2(1H)-quinolinone was used in place of 3-acetyl-4-hydroxy-2(1H)-quinolinone, 1.25 g of 1-methyl-4-hydroxy-3-[3-[3-(methoxyacetylamino)phenyl]-1-oxo-2-propenyl]-2(1H)-quinolinone [Compound No. (19e)] was obtained as a yellow crystal.

¹H-NMR (300 MHz, DMSO-d₆) δ (ppm): 3.40 (s, 3H), 3.59 (s, 3H), 4.04 (s, 2H), 7.32 (t, 1H, J=6.0 Hz), 7.40-7.50 (2H), 7.60 (d, 1H, J=6.0 Hz), 7.76-7.90 (m, 2H), 8.08 (s, 1H), 8.15 (d, 1H, J=6.0 Hz), 8.49 (d, 1H, J=15.0 Hz), 9.99 (s, 1H)

Example e-8 Synthesis of the Present Compound [Compound No. (20e)] by Process A

According to the same manner as that of Example e-3 except that 2.00 g of 4-(methoxyacetylamino)benzaldehyde was used in place of 3-(cyanomethoxy)benzaldehyde, 0.85 g of 4-hydroxy-3-[3-[4-(methoxyacetylamino)phenyl]-1-oxo-2-propenyl]-2(1H)-quinolinone [Compound No. (20e)] was obtained as a yellow crystal.

¹H-NMR (400 MHz, DMSO-d₆) δ (ppm): 3.39 (s, 3H), 4.04 (s, 2H), 7.25 (t, 1H, J=6.6 Hz), 7.31 (d, 2H, J=8.3 Hz), 7.65-7.71 (m, 1H), 7.72 (d, 1H, J=8.3 Hz), 7.82 (d, 2H, J=8.3 Hz), 7.92 (d, 1H, J=15.9 Hz), 8.02 (d, 1H, J=7.6 Hz), 8.59 (d, 1H, J=16.8 Hz), 10.07 (s, 1H), 11.48 (s, 1H)

Example e-9 Synthesis of the Present Compound [Compound No. (21e)] by Process A

According to the same manner as that of Example e-3 except that 1.12 g of 3-[(2-methoxyethoxy)carbonylamino]benzaldehyde was used in place of 3-(cyanomethoxy)benzaldehyde, 1.36 g of 4-hydroxy-3-[3-[3-[(2-methoxyethoxy)carbonylamino]phenyl]-1-oxo-2-propenyl]-2(1H)-quinolinone [Compound No. (21e)] was obtained as a yellow crystal.

¹H-NMR (400 MHz, DMSO-d₆) δ (ppm): 3.31 (s, 3H), 3.59 (t, 1H, J=4.4 Hz), 4.42 (t, 1H, J=4.8 Hz), 7.25 (t, 1H, J=7.6 Hz), 7.30-7.45 (3H), 7.58 (d, 1H, J=8.4 Hz), 7.69 (t, 1H, J=7.6 Hz), 7.87 (d, 1H, J=16.4 Hz), 7.91 (s, 1H), 8.03 (d, 1H, J=7.6 Hz), 8.62 (d, 1H, J=15.2 Hz), 9.95 (s, 1H), 11.50 (s, 1H)

Example e-10 Synthesis of the Present Compound [Compound No. (26e)] by Process A

According to the same manner as that of Example e-3 except that 0.58 g of 3-[(methanesulfonyl)aminocarbonyl]benzaldehyde was used in place of 3-(cyanomethoxy)benzaldehyde, 0.16 g of 4-hydroxy-3-[3-[3-[(methanesulfonyl)aminocarbonyl]phenyl]-1-oxo-2-propenyl]-2(1H)-quinolinone [Compound No. (26e)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 3.91 (s, 3H), 7.15-7.30 (1H), 7.32 (d, 1H, J=7.8 Hz), 7.47 (t, 1H, J=7.8 Hz), 7.60-7.85 (2H), 7.90-8.20 (3H), 8.31 (s, 1H), 8.69 (d, 1H, J=15.9 Hz), 11.56 (s, 1H), 18.12 (s, 1H)

Example e-11 Synthesis of the Present Compound [Compound No. (28e)] by Process A

According to the same manner as that of Example e-3 except that 1.64 g of 3-[[(methoxycarbonylmethyl)amino]carbonyl]benzaldehyde was used in place of 3-(cyanomethoxy)benzaldehyde, 0.39 g of 4-hydroxy-3-[3-[3-[[(methoxycarbonylmethyl)amino]carbonyl]phenyl]-1-oxo-2-propenyl]-2(1H)-quinolinone [Compound No. (28e)] was obtained as a yellow crystal.

¹H-NMR (400 MHz, DMSO-d₆) δ (ppm): 3.67 (s, 3H), 4.06 (d, 2H, J=5.9 Hz), 7.25 (t, 1H, J=7.1 Hz), 7.32 (d, 1H, J=8.3 Hz), 7.62 (t, 1H, J=7.8 Hz), 7.68 (t, 1H, J=7.1 Hz), 7.90-8.00 (m, 3H), 8.03 (d, 1H, J=7.6 Hz), 8.24 (s, 1H), 8.66 (d, 1H, J=16.6 Hz), 9.14 (t, 1H, J=5.9 Hz)

Example e-12 Synthesis of the Present Compound [Compound No. (29e)] by Process A

According to the same manner as that of Example e-3 except that 1.62 g of 4-[[(methoxycarbonylmethyl)amino]carbonyl]benzaldehyde was used in place of 3-(cyanomethoxy)benzaldehyde, 0.77 g of 4-hydroxy-3-[3-[4-[[(methoxycarbonylmethyl)amino]carbonyl]phenyl]-1-oxo-2-propenyl]-2(1H)-quinolinone [Compound No. (29e)] was obtained as a yellow crystal.

¹H-NMR (400 MHz, DMSO-d₆) δ (ppm): 3.67 (s, 3H), 4.04 (d, 1H, J=5.9 Hz), 7.26 (t, 1H, J=7.6 Hz), 7.32 (d, 1H, J=8.3 Hz), 7.70 (t, 1H, J=7.3 Hz), 7.85 (d, 2H, J=8.3 Hz), 7.90-8.00 (m, 3H), 8.03 (d, 1H, J=8.1 Hz), 8.70 (d, 1H, J=15.9 Hz), 9.08 (t, 1H, J=5.8 Hz), 11.53 (broad s, 1H)

Example e-13 Synthesis of the Present Compound [Compound No. (30e)] by Process A

According to the same manner as that of Example e-3 except that 2.13 g of 3-[(2-methoxyethyl)aminocarbonyl]benzaldehyde was used in place of 3-(cyanomethoxy)benzaldehyde, 0.74 g of 4-hydroxy-3-[3-[3-[(2-methoxyethyl)aminocarbonyl]phenyl]-1-oxo-2-propenyl]-2(1H)-quinolinone [Compound No. (30e)] was obtained as a yellow crystal.

¹H-NMR (400 MHz, DMSO-d₆) δ (ppm): 3.29 (s, 3H), 3.29-3.38 (2H), 3.48 (t, 2H, J=3.7 Hz), 7.26 (t, 1H, J=7.6 Hz), 7.33 (d, 1H, J=8.3 Hz), 7.59 (t, 1H, J=7.8 Hz), 7.70 (t, 1H, J=6.9 Hz), 7.89 (d, 1H, J=7.6 Hz), 7.94 (d, 1H, J=8.3 Hz), 7.96-8.00 (1H), 8.03 (d, 1H, J=7.3 Hz), 8.22 (s, 1H), 8.65-8.80 (1H), 11.55 (s, 1H)

Example e-14 Synthesis of the Present Compound [Compound No. (31e)] by Process A

According to the same manner as that of Example e-3 except that 0.48 g of 4-[(2-methoxyethyl)aminocarbonyl]benzaldehyde was used in place of 3-(cyanomethoxy)benzaldehyde, 0.53 g of 4-hydroxy-3-[3-[4-[(2-methoxyethyl)aminocarbonyl]phenyl]-1-oxo-2-propenyl]-2(1H)-quinolinone [Compound No. (31e)] was obtained as a yellow crystal.

¹H-NMR (400 MHz, DMSO-d₆) δ (ppm): 3.31 (s, 3H), 3.25-3.35 (2H), 3.40-3.50 (2H), 7.24 (t, 1H, J=7.8 Hz), 7.30 (d, 1H, J=8.3 Hz), 7.67 (t, 1H, J=8.0 Hz), 7.80 (d, 1H, J=8.3 Hz), 7.85-7.95 (2H), 8.01 (d, 1H, J=7.8 Hz), 8.66 (d, 1H, J=15.9 Hz)

Example e-15 Synthesis of the Present Compound [Compound No. (33e)] by Process A

According to the same manner as that of Example e-3 except that 0.73 g of 3-[[(cyanomethyl)amino]carbonyl]benzaldehyde was used in place of 3-(cyanomethoxy)benzaldehyde, 0.31 g of 4-hydroxy-3-[3-[3-[[(cyanomethyl)amino]carbonyl]phenyl]-1-oxo-2-propenyl]-2(1H)-quinolinone [Compound No. (33e)] was obtained as a yellow crystal.

¹H-NMR (400 MHz, DMSO-d₆) δ (ppm): 4.36 (d, 1H, J=5.6 Hz), 7.26 (t, 1H, J=6.8 Hz), 7.33 (d, 1H, J=7.8 Hz), 7.64 (t, 1H, J=7.6 Hz), 7.70 (t, 1H, J=8.1 Hz), 7.92-8.08 (4H), 8.24 (s, 1H), 8.68 (d, 1H, J=14.7 Hz), 9.39 (t, 1H, J=5.1 Hz)

Example e-16 Synthesis of the Present Compound [Compound No. (36e)] by Process A

According to the same manner as that of Example e-1 except that 1.0 g of 1-methyl-3-acetyl-4-hydroxy-2(1H)-quinolinone was used in place of 3-acetyl-4-hydroxy-2(1H)-quinolinone, 1.42 g of 1-methyl-4-hydroxy-3-[3-[3-[(methoxycarbonyl)methoxy]phenyl]-1-oxo-2-propenyl]-2(1H)-quinolinone [Compound No. (36e)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 3.61 (s, 3H), 3.74 (s, 3H), 4.89 (s, 2H), 7.06-7.10 (m, 1H), 7.30-7.45 (m, 4H), 7.58 (d, 1H, J=8.1 Hz), 7.81-7.92 (m, 2H), 8.15-8.18 (m, 1H), 8.57 (d, 1H, J=15.7 Hz), 17.72 (broad s, 1H)

Example e-17 Synthesis of the Present Compound [Compound No. (37e)] by Process C

According to the same manner as that of Example e-2 except that 0.25 g of 1-methyl-4-hydroxy-3-[3-[3-[(methoxycarbonyl)methoxy]phenyl]-1-oxo-2-propenyl]-2(1H)-quinolinone was used in place of 4-hydroxy-3-[3-[3-[(methoxycarbonyl)methoxy]phenyl]-1-oxo-2-propenyl]-2(1H)-quinolinone, 0.18 g of 1-methyl-4-hydroxy-3-[3-[3-(carboxymethoxy)phenyl]-1-oxo-2-propenyl]-2(1H)-quinolinone [Compound No. (37e)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 3.60 (s, 3H), 4.75 (s, 2H), 7.05 (d, 1H, J=6.8 Hz), 7.28-7.58 (m, 5H), 7.79-7.92 (m, 2H), 8.15 (d, 1H, J=8.1 Hz), 8.57 (d, 1H, J=15.4 Hz)

Example e-18 Synthesis of the Present Compound [Compound No. (60e)] by Process B

To a mixture of 0.55 g of 1-methyl-4-hydroxy-3-[3-[3-[(methoxycarbonyl)methoxy]phenyl]-1-oxo-2-propenyl]-2(1H)-quinolinone and 5 ml of hexamethylphosphoramide was added 67 mg of sodium hydride (60% oily) under ice-cooling. After stirred at room temperature for 1 hour, 0.2 ml of dimethyl sulfate was added, and this was stirred at room temperature for 3 hours. The reaction solution was poured into water, followed by extraction with ethyl acetate. After dried over anhydrous sodium sulfate, the extract was concentrated under reduced pressure, and the resulting residue was subjected to silica column gel chromatography to obtain 0.21 g of 1-methyl-4-methoxy-3-[3-[3-[(methoxycarbonyl)methoxy]phenyl]-1-oxo-2-propenyl]-2(1H)-quinolinone [Compound No. (60e)] as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 3.60 (s, 3H), 3.69 (s, 3H), 3.90 (s, 3H), 4.85 (s, 2H), 6.99-7.03 (m, 1H), 7.20 (d, 1H, J=15.9 Hz), 7.30-7.38 (m, 4H), 7.53 (d, 1H, J=15.9 Hz), 7.60 (d, 1H, J=8.6 Hz), 7.70-7.77 (m, 1H), 7.99 (d, 1H, J=7.6 Hz)

Example e-19 Synthesis of the Present Compound [Compound No. (64e)] by Process C

According to the same manner as that of Example e-2 except that 0.21 g of 1-methyl-4-methoxy-3-[3-[3-[(methoxycarbonyl)methoxy]phenyl]-1-oxo-2-propenyl]-2(1H)-quinolinone was used in place of 4-hydroxy-3-[3-[3-[(methoxycarbonyl)methoxy]phenyl]-1-oxo-2-propenyl]-2(1H)-quinolinone, 0.14 g of 1-methyl-4-methoxy-3-[3-[3-(carboxymethoxy)phenyl]-1-oxo-2-propenyl]-2(1H)-quinolinone [Compound No. (64e)] was obtained as a yellow crystal.

¹H-NMR (270 MHz, DMSO-d₆) δ (ppm): 3.60 (s, 3H), 3.90 (s, 3H), 4.73 (s, 2H), 6.97-7.01 (m, 1H), 7.18 (d, 1H, J=16.3 Hz), 7.32-7.38 (m, 4H), 7.53 (d, 1H, J=16.3 Hz), 7.60 (d, 1H, J=8.4 Hz), 7.70-7.77 (m, 1H), 7.97-8.01 (m, 1H), 12.98 (broad s, 1H)

Example f-1 Synthesis of the Present Compound [Compound No. (11f)] by Process A

According to the same manner as that of Example e-3 except that 92 mg of 3-(3-hydroxypropoxy)benzaldehyde was used in place 3-(cyanomethoxy)benzaldehyde, 49 mg of 4-(1-piperidino)-3-[3-[3-(3-hydroxypropoxy)phenyl]-1-oxo-2-propenyl]-2(1H)-quinolinone [Compound No. (11f)] was obtained as a yellow crystal.

¹H-NMR (400 MHz, CDCl₃) δ (ppm): 1.78-2.04 (m, 9H), 3.82-4.13 (m, 8H), 6.90-6.92 (m, 2H), 7.03-7.13 (m, 4H), 7.23-7.39 (m, 3H), 7.91 (broad s, 1H), 8.14-8.16 (m, 1H)

Example 3 Preparation of a Plasmid Having a Reporter Gene Linked to a Transcription Regulatory Region for a Type I Collagen Gene

1×10⁸ cells of a normal human fetal skin fibroblast (Clontech, catalogue No. CC-2509) were cultured at 37° C. overnight under 5% CO₂ atmosphere. After the cultured cells were washed with a sodium phosphate buffer (hereinafter, referred to as PBS) twice, 3 ml of PBS was added thereto and the cells were scraped away the wall of a vessel using a cell scraper (Nalgen, catalogue No. 179693). The scraped cells were collected by centrifugation (1,500 rpm, 4° C., 15 min), and these were suspended in 20 ml of PBS and centrifuged again. To the resulting precipitates were added 11 ml of Solution 2 and 4.8 μl of pronase of DNA Extraction Kit (Stratagene, catalogue No. 200600). After shaken at 60° C. for 1 hour, the resulting mixture was allowed to stand in ice for 10 minutes. Then, 4 ml of Solution 3 of the kit was added to the mixture. After mixed, the mixture was allowed to stand in ice for 5 minutes and then centrifuged (3,000 rpm, 4° C., 15 min) to recover a supernatant. To the recovered supernatant was added 2 μl of RNase per 1 ml of the supernatant and the mixture was allowed to stand at 37° C. for 15 minutes. To the mixture was added 2-fold volume of ethanol. After mixed, a white thread-like substance (genomic DNA) appeared and the substance was recovered. The recovered genomic DNA was washed with 70% ethanol and then air-dried. The air-dried genomic DNA was dissolved in 500 μl of 10 mM Tris-HCl, 1 mM EDTA (pH 8.0) (hereinafter, referred to as TE).

The resulting genomic DNA solution (the amount equivalent to 1 μg of genomic DNA), each 1 μl (10 pmol/μl) of an oligonucleotide consisting of the nucleotide sequence represented by SEQ ID No:1 and an oligonucleotide consisting of the nucleotide sequence represented by SEQ ID No:2, 29 μL of distilled water, 5 μl of the buffer attached to TaKaRa LA Taq (TAKARA SHUZO, catalogue No. RR002A), 5 μL of a Mg²⁺ solution, 5 μL of a dNTP mixture and 0.5 μl of TaKaRa LA Taq (TAKARA SHUZO, catalogue No. RR002A) were mixed. After the resulting mixed solution was incubated at 94° C. for 5 minutes, the mixed solution was subjected to 30 cycles, in which one cycle consists of incubation at 94° C. for 1 minute, at 60° C. for 1 minute and then at 72° C. for 1 minute. The mixed solution was electrophoresed on a 2% agarose gel to recover about 0.5 kb of a DNA. The recovered DNA was treated with phenol/chloroform and then precipitated with ethanol to recover the DNA. The resulting DNA was dissolved in ultrapure water. To this solution were added 2.5 μl of NheI and 2.5 μl of HindIII, and then incubated at 37° C. for 3 hours. Then, the solution was electrophoresed on a 2% agarose gel to recover about 3.5 kb of a DNA. The recovered DNA was precipitated with ethanol to recover again the DNA (hereinafter, referred to as the collagen promoter DNA).

On the other hand, the vector pGL3 (Promega, catalogue No. E1751) having the nucleotide sequence encoding firefly luciferase was digested with NheI and HindIII, and then subjected to agarose gel electrophoresis as described above to recover about 5 kb of a DNA. The recovered DNA was precipitated with ethanol to recover the DNA again. To the recovered DNA were added 44 μl of distilled water, 5 μl of Buffer attached to Alkaline Phosphatase (TAKARA SHUZO, catalogue No. 2120A) and 1 μl of Alkaline Phosphatase (TAKARA SHUZO, catalogue No. 2120A). The mixed solution was incubated at 65° C. for 30 minutes. Then, the mixed solution was treated with phenol/chloroform twice, and precipitated with ethanol to recover the DNA (hereinafter referred to as the Luc vector DNA). Then, after about 20 ng of the collagen promoter DNA and about 20 ng of the Luc vector DNA were mixed, the same amount of a DNA Ligation kit Ver2 enzyme solution was added and this was incubated overnight at 16° C. To the mixed solution was added Escherichia coli 5Hdα (TOYOBO, catalogue No. DNA-903), this was allowed to stand in ice for 30 minutes, and then incubated at 42° C. for 45 seconds. The resulting Escherichia coli was seeded on a LB plate containing 50 μg/ml ampicillin sodium (Nacalai, catalogue No. 027-39), and this was allowed to stand at 37° C. for 1 day. A single colony appeared and the colony was cultured in 2 ml of a LB medium containing 50 μg/ml ampicillin at 37° C. for 12 hours. From the resulting culture solution, a plasmid DNA was prepared using AUTOMATIC DNA ISOLATION SYSTEM PI-50 (KURABO). The nucleotide sequence of the prepared plasmid DNA was analyzed with a DNA sequencer. As a result, it was confirmed that the plasmid (hereinafter, referred to as COL-Luc) had a nucleotide sequence comprising a nucleotide sequence encoding the amino acid sequence of firefly luciferase as a reporter gene linked downstream of the nucleotide sequence −3500 to +57 (the transcription initiation point is +1) of a transcription regulatory region for a human-derived Type I collagen α2 chain gene.

Example 4 Measurement of the Ability of a Test Compound to Regulate Transcription of a Type I Collagen Gene Using the Expression Level of a Report Gene as an Index

1×10⁶ cells of a normal human fetal skin fibroblast were seeded on a 100 mm dish and cultured at 37° C. overnight under 5% CO₂ atmosphere in a Dulbecco's-MEM (Nissui Seiyaku, catalogue No. 05919) medium containing 10(v/v) % heat-inactivated bovine fetal serum (hereinafter, referred to as FBS; Gibco, catalogue No. 21140-079) (hereinafter, this medium is referred to as D-MEM(+)). Then, the medium was replaced with a Dulbecco's-MEM medium not containing FBS (hereinafter, this medium is referred to as D-MEM(−)).

To 300 μl of D-MEM(−) were added 5 μg of COL-Luc and 5 μg of pCMV-β-gal (Invitrogen, catalogue No. 10586-014), and the resulting mixed solution was allowed to stand at room temperature for 5 minutes (solution 1). To 300 μl of D-MEM(−) was added 20 μl of Lipofectine (Gibco, catalogue No. 18292-011), and the resulting mixed solution was allowed to stand at room temperature for 45 minutes (solution 2). Then, the solution 1 and the solution 2 were mixed. After the mixture was allowed to stand at room temperature for 10 minutes, 5.4 ml of D-MEM(−) was added to thereto, followed by mixing. The mixed solution was added to the normal human fetal skin fibroblasts, and the cells were cultured at 37° C. under 5% CO₂ atmosphere. After 6 hours, the culture supernatant was removed from the dish, and the cells were washed with PBS twice. To the dish was added 1 ml of PBS containing 0.25% trypsin, and the cells were scraped off the dish. To the scraped cells was added D-MEM(+), and these were mixed well. The mixture was dispensed into a 12-well plate at 1 ml per well, and the plate was incubated at 37° C. overnight under 5% CO₂ atmosphere. On the next day, each well was washed with D-MEM(−) twice, and this was replaced with 1 ml of a Dulbecco's-MEM medium containing 0.1% FBS (hereinafter, this medium is referred to as D-MEM (0.1%)).

To the thus cultured cells was added 10 μl of a 100 μM solution of the present compound represented by the compound number (7a) to (11a), (13a), (18a) to (20a), (22a), (28a) to (35a), (10e), (11e), (13e), (18e) to (21e), (26e), (28e) to (31e) or (33e) in dimethyl sulfoxide (hereinafter, DMSO) (final concentration 1 μM). As a control, only 10 μl of DMSO was added.

After one hour, 10 μl of a 0.5 μg/ml aqueous solution of TGF-β (Pepro Tech) or distilled water was added to the well, and the plate was further incubated at 37° C. for 40 hours under 5% CO₂ atmosphere. After the incubated cells were washed with PBS twice, 200 μl of a cell lysing agent (Toyo Inc., catalogue No. PD10) was added thereto and the cells were scraped. The scraped cells were recovered as a cell suspension, and the suspension was centrifuged (15,000 rpm, 4° C., 5 min) to recover a supernatant. The recovered supernatant was transferred to a 96-well plate at 50 μl per well, and then 50 μl of a Luc assay solution (20 mM Tricine (pH 7.8), 2.67 mM MgSO₄, 0.1 mM EDTA, 33.3 mM DTT, 270 μM Coenzyme A, 530 μM ATP, 470 μM Luciferin) was automatically dispensed into the plate using MICROLUMAT LB96P (manufactured by EG&G BERTHOLD). Luminescence in each well was measured (Delay: 1.6 second, Meas. Interval: 20 second).

On the other hand, 50 μl of the recovered supernatant or the cell lysing agent was added to 50 μl of a β-gal substrate solution (5.8 mM o-nitrophenyl-beta-D-galactopyranoside, 1 mM MgCl₂, 45 mM 2-mercaptoethanol) which had been dispensed into a 96-well plate in advance, and the plate was incubated at 37° C. for 2 hours. Then, an absorbance in each well was measured using a microplate reader at 420 nm. Based on the resulting value, the transcription activity was calculated according to the following equation: Transcription activity=[luminescence amount (supernatant-added section)−luminescence amount (cell lysing agent-added section)]/[420 nm absorbance (supernatant-added section)−420 nm absorbance (cell lysing agent-added section)]

Then, based on the calculated transcription activity, an inhibitory effect of a test compound on the ability of TGF-β to promote transcription of a Type I collagen gene was calculated as an inhibition percentage according to the following equation: Inhibition percentage=[transcription activity (DMSO and TGF-β-added test section)−transcription activity (compound and TGF-β-added test section)]/[transcription activity (DMSO and TGF-β-added test section)−transcription activity (DMSO and TGF-β non-added test section)]×100

The inhibition percentages of the present compounds represented by the compound number (7a) to (11a), (13a), (18a) to (20a), (22a), (28a) to (35a), (10e), (11e), (13e), (18e) to (21e), (26e), (28e) to (31e) and (33e) were 70 or more. It was found that these compounds can inhibit the ability of TGF-β to promote transcription of a Type I collagen gene, and then can suppress transcription of a Type I collagen gene.

Example 5 Improvement of Chronic Renal Failure by Administration of the Present Compound

(1) Preparation of Anti-Thy-1 Antibody (IgG)

IgG was purified from an ascites-lyophilized powder (CEDARLANE, lot No. 05122) containing an anti-rat CD90 (Thy1.1) monoclonal antibody using MAbTrap kit (Amersham Biosciences, catalogue No. 17-1128-01).

6 ml of a binding buffer was added to 3 ml of ascites to sufficiently recover it, and this was passed through a 0.22 μm filter. The resulting solution was applied to a pre-bufferized column, and the column was then washed with 10 ml of a binding buffer. Thereafter, the column was eluted with 5 ml of an elution buffer. From washing, each 1 ml was fractionated, and the protein concentration of each fraction was measured using bovine serum albumin as a standard. A single peak was confirmed from an elution pattern, and an IgG fraction was dialyzed against a physiological saline overnight at 4° C. The protein concentration of the resulting anti-Thy-1 antibody (IgG) was calculated.

(2) Administration of Anti-Thy-1 Antibody (IgG) and Compound

The present compounds represented by the compound numbers (28a) (hereinafter, referred to as the present compound (28a)), the present compound represented by a compound number (30a) (hereinafter, referred to as the present compound (30a)), the present compound represented by a compound number (33a) (hereinafter, referred to as the present compound (33a)), the present compound represented by a compound number (10e) (hereinafter, referred to as the present compound (10e)), the present compound represented by a compound number (18e) (hereinafter referred to as the present compound (18e)), the present compound represented by a compound number (31e) (hereinafter, referred to as the present compound (31e)) and a corn oil which is a medium were weighed. These were mixed using a mortar and a pestle to prepare a 3 mg/kg solution. Using four 7-week old male Wistar rats [Nippon Charles River] per group, 60 μg/ml of the anti-Thy-1 antibody (IgG) or a physiological saline was intravenously injected via a tail vein at 5 ml/kg. Immediately after administration of the anti-Thy-1 antibody (IgG) or a physiological saline, the present compound or a corn oil was repeatedly orally administered for 7 days at 5 ml/kg. A dose of the present compound was 15 mg/kg/day.

(3) Quantization of mRNA of Type I Collagen Gene in Renal Glomerulus

On the next day of final administration, a rat reared as described in the above (2) was slaughtered by collection of whole blood, and kidneys were isolated. A whole RNA was separated from the cortex of the isolated kidney using RNeasy Mini Kit (QIAGEN, catalogue No. 74106). To 5 μl (50 ng) of the separated whole RNA were added 1 μl of 20 μM oligo dT and 4 μl of RNase-free distilled water. After the mixture was incubated at 65° C. for 5 minutes, it was immediately ice-cooled. To 10 μl of the solution were added 4 μl of 5× buffer, 2.4 μl of MgCl₂, 1 μl of 10 mM dNTP, 1 μl of RNasin, 1 μl of Improm II and 0.6 μl of RNase-free distilled water (all available from Promega), and a reverse transcription reaction was performed under the condition of at 25° C. for 5 minutes, at 42° C. for 1 hour, and at 70° C. for 15 minutes.

Into 5 μl of the reverse transcription reaction solution were mixed each 2 μl of each 1.25 pmol/μl of primers represented by SEQ ID NOS: 3 and 4, 1.25 μl of a probe (FAM-ctcgccttca tgcgcctgct agc-TAMRA) for detecting DNA of a Type I collagen gene represented by SEQ ID No:5, each 0.25 μl of Rodent GAPDH primers, 0.25 μl of a Rodent GAPDH probe, 12.5 μl of TaqMan Universal PCR Master Mix (all available from Applied Biosystem) and 1.5 μl of sterilized water in a well of Optical 96-Well Reaction Plate (Applied Biosystem, catalogue No. N801-0560). As a standard, in place of 5 μl of the reverse transcription reaction solution, each 5 μl of 500, 250, 125, 62.5, 31.25, 15.625 ng/μl rat renal cortex cDNA which had been prepared in advance was used. Thereafter, using Gene Amp 7900 (Applied Biosystem), PCR was performed under the condition of at 50° C. for 5 minutes, and 40 cycles in which one cycle is at 95° C. for 15 seconds and at 60° C. for 1 minute. For quantization, a standard straight line was made, the Type I collagen and GAPDH amounts of each sample were calculated, and the transcription amount was calculated according to the following equation: Type I collagen transcription amount=Type I collagen amount/GAPDH amount

For statistical treatment of the resulting result, F-test of a variance ratio was performed between two groups of the anti-Thy-1 antibody and corn oil-administered groups and other each group. When there is no significant difference in variance, Student test (one-tailed) was performed and, when there is a significant difference in variance, Aspin-Welch test (one-tailed) was performed. Results are shown in Table 3 and Table 4.

It was found that the present compound (28a), the present compound (30a), the present compound (33a), the present compound (10e), the present compound (18e) and the present compound (31e) have the ability to improve chronic renal failure.

TABLE 3 Anti- mRNA of Thy-1 Administrated collagen Test Group antibody substance gene results Control group + Corn oil 3.4 — Present + Present 2.0 p < 0.05 compound(28a)- compound administered (28a) group Present + Present 2.4 p < 0.05 compound(30a)- compound administered (30a) group Present + Present 2.4 p < 0.05 compound(33a)- compound administered (33a) group Normal group − Corn oil 1.9 p < 0.01

TABLE 4 Anti- mRNA of Thy-1 Administrated collagen Test Group antibody substance gene results Control group + Corn oil 5.6 — Present + Present 2.0 p < 0.01 compound(10e)- compound administered (10e) Present + Present 1.9 p < 0.01 compound(18e)- compound administered (18e) Present + Present 3.3 p < 0.01 compound(31e)- compound administered (31e) Normal group − Corn oil 1.7 p < 0.01

Example 6 Improvement of Chronic Renal Failure by Administration of the Present Compound

(1) Preparation of Anti-Thy-1 Antibody (IgG)

Preparation was performed as described in Example 5.

(2) Administration of Anti-Thy-1 Antibody (IgG) and Compound

According to the same manner as that of Example 5 except that the present compound represented by the compound number (37a) (hereinafter, referred to as the present compound (37a)) was used as the present compound, a 0.5% aqueous methylcellulose solution was used as a medium, and 10 Wistar rats were used per group, administration was performed.

(3) Quantitation of mRNA of Type I Collagen Gene in Renal Glomerulus

Quantitation was performed as descried in Example 5. Results are shown in Table 5.

It was found that the present compound (37a) has the ability to improve chronic renal failure.

TABLE 5 Anti- mRNA of Thy-1 Administrated collagen Test Group antibody substance gene results Control + 0.5% aqueous 2.1 — group methylcellulose solution Present + Present 1.5 p < 0.01 compound compound (37) (37a)- administered group Normal group − 0.5% aqueous 1.0 p < 0.01 methylcellulose solution

INDUSTRIAL APPLICABILITY

According to the present invention, it is possible to develop and provide a composition which decreases expression of a Type I collage gene in a tissue to induce a reduction in accumulation of collagen and thereby improves tissue fibrosis (i.e. a collagen accumulation-suppressing agent and a fibrosing disease-treating agent).

SEQUENCE LISTING FREE TEXT

SEQ ID NO: 1

Oligonucleotide primer designed for amplifying a collagen promoter DNA

SEQ ID NO: 2

Oligonucleotide primer designed for amplifying a collagen promoter DNA

SEQ ID NO: 3

Oligonucleotide primer designed for detecting a collagen DNA

SEQ ID NO: 4

Oligonucleotide primer designed for detecting a collagen DNA

SEQ ID NO: 5

Oligonucleotide probe designed for detecting a collagen DNA 

1. A 2(1H)-pyridinone compound represented by the formula (XI):

wherein: X_(I)′ represents a C2-C4 alkenyl group substituted with a cyano group, an A_(I)′—R_(I)—O— group (wherein A_(I)′ represents a C1-C4 alkylthio group, a C2-C4 alkenyl group, a C2-C4 alkynyl group, a C1-C4 alkoxycarbonyl group, a carboxy group or a cyano group, and R_(I) represents a C1-C4 alkylene group), an A_(II)-(y)_(m)-z-NH— group (wherein A_(II) represents a C2-C4 alkenyl group, or a C1-C4 alkyl group substituted with a C1-C4 alkoxy group, a C1-C4 alkoxycarbonyl group, a carboxy group or a cyano group, y represents an oxy group or an imino group, z represents a carbonyl group or a sulfonyl group, and m represents 0 or 1), or an A_(III)-NHCO— group (wherein A_(III) represents a methanesulfonyl group, or a C1-C4 alkyl group substituted with a hydroxy group, a C1-C4 alkoxy group, a C1-C4 alkoxycarbonyl group, a carboxy group or a cyano group); a and b are the same or different, and represent a hydrogen atom or a C1-C4 alkyl group; Y₁ represents a halogen atom, a nitro group, a C1-C4 alkyl group or a C1-C4 alkoxy group; n represents 0, 1 or 2, and when n is 2, Y_(I)s may be different.
 2. A 2(1H)-pyridinone compound represented by the formula (XIII):

wherein: X_(II) represents a carboxymethoxy group, a dimethylaminocarbonylmethoxy group, a 3-dimethylaminopropoxy group, a 2-hydroxyethoxy group, a cyanomethoxy group, a methoxyacetylamino group, a 2-methoxyethoxycarbonylamino group, a 2-methoxyethylaminocarbonyl group or a methoxycarbonylmethylaminocarbonyl group, and a′ and b′ are the same or different, and represent a hydrogen atom or a methyl group.
 3. A 2(1H)-pyridinone compound represented by the formula (XV):


4. A 2(1H)-pyridinone compound represented by the formula (XVI):


5. A 2(1H)-pyridinone compound represented by the formula (XVII):


6. A 2(1H)-pyridinone compound represented by the formula (XVIII):


7. A 2(1H)-pyridinone compound represented by the formula (XIX):


8. A 2(1H)-pyridinone compound represented by the formula (XX):


9. A 2(1H)-pyridinone compound represented by the formula (XXI):


10. A 2(1H)-pyridinone compound represented by the formula (XXII):


11. A 2(1H)-pyridinone compound represented by the formula (XXIII):


12. A 2(1H)-pyridinone compound represented by the formula (XXIV):


13. A 2(1H)-pyridinone compound represented by the formula (XXV):


14. A 2(1H)-pyridinone compound represented by the formula (XXVI):


15. A 2(1H)-pyridinone compound represented by the formula (XXVII):


16. A 2(1H)-pyridinone compound represented by the formula (XXVIII):


17. A 2(1H)-pyridinone compound represented by the formula (XXIX)


18. A 2(1H) pyridinone compound represented by the formula (XXX):


19. A composition for improving tissue fibrosis, which comprises a compound according to claim 1 and an inert carrier.
 20. A method for improving tissue fibrosis, which comprises administering an effective amount of a compound according to claim 1 to a mammal in need thereof.
 21. A composition for suppressing the activity of TGF-β, which comprises a compound according to claim 1 and an inert carrier.
 22. A method for treating chronic renal failure, which comprises administering an effective amount of a compound according to claim 1 to a mammal in need thereof.
 23. A composition for suppressing transcription of a Type I collagen gene, which comprises a compound according to claim 1 and an inert carrier.
 24. A composition for suppressing transcription of a Type I collagen gene, which comprises a compound according to claim 2 and an inert carrier.
 25. A composition for suppressing transcription of a Type I collagen gene, which comprises a compound according to claim 3 and an inert carrier. 